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Schematic description of the experimental conditions for electrophysiology. (A) Timeline of the electrophysiological recordings in the <t>multielectrode</t> array system. Three baseline recordings with aCSF and a solution of 200 nM of 4-AP were followed by 10 min of a continuous administration of a 2 μM solution of either D1 or D2 agonist. (B) Example of the <t>MEA</t> recording system with a slice positioned to ensure that the areas of interest (S1 and S2) contact the recording electrodes. (C) Schematic of the labeling of electrodes using the Paxinos mouse brain atlas as a reference.
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Schematic description of the experimental conditions for electrophysiology. (A) Timeline of the electrophysiological recordings in the <t>multielectrode</t> array system. Three baseline recordings with aCSF and a solution of 200 nM of 4-AP were followed by 10 min of a continuous administration of a 2 μM solution of either D1 or D2 agonist. (B) Example of the <t>MEA</t> recording system with a slice positioned to ensure that the areas of interest (S1 and S2) contact the recording electrodes. (C) Schematic of the labeling of electrodes using the Paxinos mouse brain atlas as a reference.
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Schematic description of the experimental conditions for electrophysiology. (A) Timeline of the electrophysiological recordings in the <t>multielectrode</t> array system. Three baseline recordings with aCSF and a solution of 200 nM of 4-AP were followed by 10 min of a continuous administration of a 2 μM solution of either D1 or D2 agonist. (B) Example of the <t>MEA</t> recording system with a slice positioned to ensure that the areas of interest (S1 and S2) contact the recording electrodes. (C) Schematic of the labeling of electrodes using the Paxinos mouse brain atlas as a reference.
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Schematic description of the experimental conditions for electrophysiology. (A) Timeline of the electrophysiological recordings in the <t>multielectrode</t> array system. Three baseline recordings with aCSF and a solution of 200 nM of 4-AP were followed by 10 min of a continuous administration of a 2 μM solution of either D1 or D2 agonist. (B) Example of the <t>MEA</t> recording system with a slice positioned to ensure that the areas of interest (S1 and S2) contact the recording electrodes. (C) Schematic of the labeling of electrodes using the Paxinos mouse brain atlas as a reference.
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Schematic description of the experimental conditions for electrophysiology. (A) Timeline of the electrophysiological recordings in the <t>multielectrode</t> array system. Three baseline recordings with aCSF and a solution of 200 nM of 4-AP were followed by 10 min of a continuous administration of a 2 μM solution of either D1 or D2 agonist. (B) Example of the <t>MEA</t> recording system with a slice positioned to ensure that the areas of interest (S1 and S2) contact the recording electrodes. (C) Schematic of the labeling of electrodes using the Paxinos mouse brain atlas as a reference.
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Schematic description of the experimental conditions for electrophysiology. (A) Timeline of the electrophysiological recordings in the <t>multielectrode</t> array system. Three baseline recordings with aCSF and a solution of 200 nM of 4-AP were followed by 10 min of a continuous administration of a 2 μM solution of either D1 or D2 agonist. (B) Example of the <t>MEA</t> recording system with a slice positioned to ensure that the areas of interest (S1 and S2) contact the recording electrodes. (C) Schematic of the labeling of electrodes using the Paxinos mouse brain atlas as a reference.
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Schematic description of the experimental conditions for electrophysiology. (A) Timeline of the electrophysiological recordings in the <t>multielectrode</t> array system. Three baseline recordings with aCSF and a solution of 200 nM of 4-AP were followed by 10 min of a continuous administration of a 2 μM solution of either D1 or D2 agonist. (B) Example of the <t>MEA</t> recording system with a slice positioned to ensure that the areas of interest (S1 and S2) contact the recording electrodes. (C) Schematic of the labeling of electrodes using the Paxinos mouse brain atlas as a reference.
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Schematic description of the experimental conditions for electrophysiology. (A) Timeline of the electrophysiological recordings in the <t>multielectrode</t> array system. Three baseline recordings with aCSF and a solution of 200 nM of 4-AP were followed by 10 min of a continuous administration of a 2 μM solution of either D1 or D2 agonist. (B) Example of the <t>MEA</t> recording system with a slice positioned to ensure that the areas of interest (S1 and S2) contact the recording electrodes. (C) Schematic of the labeling of electrodes using the Paxinos mouse brain atlas as a reference.
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Schematic description of the experimental conditions for electrophysiology. (A) Timeline of the electrophysiological recordings in the multielectrode array system. Three baseline recordings with aCSF and a solution of 200 nM of 4-AP were followed by 10 min of a continuous administration of a 2 μM solution of either D1 or D2 agonist. (B) Example of the MEA recording system with a slice positioned to ensure that the areas of interest (S1 and S2) contact the recording electrodes. (C) Schematic of the labeling of electrodes using the Paxinos mouse brain atlas as a reference.

Journal: Frontiers in Neuroanatomy

Article Title: Chronic silencing of Drd1a-Cre+ neurons impairs dopaminergic-driven cortical activation

doi: 10.3389/fnana.2025.1548545

Figure Lengend Snippet: Schematic description of the experimental conditions for electrophysiology. (A) Timeline of the electrophysiological recordings in the multielectrode array system. Three baseline recordings with aCSF and a solution of 200 nM of 4-AP were followed by 10 min of a continuous administration of a 2 μM solution of either D1 or D2 agonist. (B) Example of the MEA recording system with a slice positioned to ensure that the areas of interest (S1 and S2) contact the recording electrodes. (C) Schematic of the labeling of electrodes using the Paxinos mouse brain atlas as a reference.

Article Snippet: We evaluated the role of the Drd1a-Cre+ neurons in the maintenance and activation of dopaminergic networks in somatosensory cortex using a multielectrode array (MEA) (MaxOne, Maxwell Biosystems).

Techniques: Labeling