Journal: Frontiers in Surgery

Article Title: Fluorescein-distribution in confocal laser endomicroscopy allows for discrimination between primary brain tumours and metastases

doi: 10.3389/fsurg.2025.1567711

Figure Lengend Snippet: Time-dependent fluorescence intensity and representative microscopy images. (a) Mean fluorescence intensity in Ln229 cells as a function of incubation time. Error bars indicate the 95% confidence interval. (b–g) Fluorescent images of a Ln229 cell monolayer stained with SF (b,d) , PI (c,f) and Hoechst (d,g) for 40 min. Exposure time was adjusted for each picture individually. Dynamic display range was set to the minimum/maximum non-zero value for the green and DAPI channels; for the red channel the maximum pixel value was set to the camera's maximum pixel value of 16,384. White bar ∼50 μm. (a–d) Incubation of living cells, (e,f) Incubation of fixed cells.

Article Snippet: These specimens were snap frozen, sliced on a cryostat, stained with conventional Hematoxylin & Eosin (H&E) and on the corresponding following slide, conventional fluorescence microscopy was performed to detect the SF fluorescence with a multichannel fluorescence microscopy (Imager Z2, Carl Zeiss Meditec AG).

Techniques: Fluorescence, Microscopy, Incubation, Staining

Journal: Frontiers in Surgery

Article Title: Fluorescein-distribution in confocal laser endomicroscopy allows for discrimination between primary brain tumours and metastases

doi: 10.3389/fsurg.2025.1567711

Figure Lengend Snippet: Comparison of fluorescence intensity between different cell lines after SF stain. (a) Differential fluorescence intensity per cell line. Dotted pattern: glioma cell lines, striped pattern: adenocarcinoma cell lines. Error bars indicate the 95% confidence interval. *indicates a significance level of 0.05. For cell lines Ln18, T98G, and Ln229 differences to all of the adenocarcinoma were significant at the 0.05 level. (b–i) Representative fluorescence microscopy images of the stained cell lines: (b) Ln18, (c) T98G, (d) Ln229, (e) U87MG, (f) MCF7, (g) SW48, (h) MDA, and (i) OV-MZ-6. Scale bars: 25 µm.

Article Snippet: These specimens were snap frozen, sliced on a cryostat, stained with conventional Hematoxylin & Eosin (H&E) and on the corresponding following slide, conventional fluorescence microscopy was performed to detect the SF fluorescence with a multichannel fluorescence microscopy (Imager Z2, Carl Zeiss Meditec AG).

Techniques: Comparison, Fluorescence, Staining, Microscopy

Journal: Frontiers in Surgery

Article Title: Fluorescein-distribution in confocal laser endomicroscopy allows for discrimination between primary brain tumours and metastases

doi: 10.3389/fsurg.2025.1567711

Figure Lengend Snippet: (A) resected metastasis after intravenous application of SF in conventional fluorescence microscopy. Scale bar: 50 µm. (B) H&E staining of the same metastasis as a comparison. Scale bar: 50 µm.

Article Snippet: These specimens were snap frozen, sliced on a cryostat, stained with conventional Hematoxylin & Eosin (H&E) and on the corresponding following slide, conventional fluorescence microscopy was performed to detect the SF fluorescence with a multichannel fluorescence microscopy (Imager Z2, Carl Zeiss Meditec AG).

Techniques: Fluorescence, Microscopy, Staining, Comparison