Journal: bioRxiv

Article Title: Dopamine-induced Relaxation of Connectivity Diversifies Burst Patterns in Cultured Hippocampal Networks

doi: 10.1101/2024.06.26.600923

Figure Lengend Snippet: D o pamine Experiment on Hippocampal Cultures Recorded by MaxOne Chip A: The experimental scheme. B: Raster plots depicting spiking activity of active electrodes for the representative sample at baseline level (top) and at four levels of dopamine (DA) concentration (second to bottom, 30, 100, 300, 1000 μM). Note that we used this representative sample to illustrate subsequent analyses (see - ).

Article Snippet: MaxOne high-density multi-electrode array (HD-MEA) chips (Maxwell Biosystems, Zurich, Switzerland) were pretreated and coated according to the manufacturer’s protocol.

Techniques: Activity Assay, Concentration Assay

Journal: bioRxiv

Article Title: Dopamine-induced Relaxation of Connectivity Diversifies Burst Patterns in Cultured Hippocampal Networks

doi: 10.1101/2024.06.26.600923

Figure Lengend Snippet: E f fects of dopamine on network clusters and their connectivity A-B: Spatial profiles of the presentative sample in the baseline condition (A) and 1000 μM condition (B). Each dot represents the relative position of a single electrode in the MaxOne high-density microelectrode array. Colours represent the modularity, where electrodes in the same module share the same colour. Lines connect the electrode pairs whose connectivity strength was greater than a threshold, defined by the top 1% in the baseline condition. Coloured thin lines connect electrodes in the same module and black thick lines connect electrodes in different modules. C: Histograms of participation coefficients of the electrodes in the baseline condition (blue bars) and 1000 μM condition (orange bars) for the representative sample. D: Distance of electrode pairs in the two pools of within-module and across-module for the baseline condition (grey bars) and the four DA conditions (red shaded bars). E-F: Changes in number of modules (E) and the mean participation coefficient (F), normalised by the baseline level, for the four DA conditions, n=8 samples. The convention was the same as in . * p < 0.05, ** p < 0.01

Article Snippet: MaxOne high-density multi-electrode array (HD-MEA) chips (Maxwell Biosystems, Zurich, Switzerland) were pretreated and coated according to the manufacturer’s protocol.

Techniques: Microelectrode Array