Journal: bioRxiv

Article Title: Consumption of processed foods impairs memory function through dietary advanced glycation end-products

doi: 10.64898/2026.01.07.698065

Figure Lengend Snippet: a, mRNA expression of RAGE in the HPCd (n=8 CTL, n=8 AGE-rich; unpaired two-tailed t-test, P=0.3075). b, mRNA expression of NFĸB in the HPCd (n=8 CTL, n=7 AGE-rich; unpaired two-tailed t-test, P=0.4285). c, mRNA expression of PSD95 in the HPCd (n=8 CTL, n=7 AGE-rich; unpaired two-tailed t-test, P=0.0532). d, mRNA expression of occludin in the duodenum (n=8 CTL, n=6 AGE-rich; unpaired two-tailed t-test, P=0.8278). e, mRNA expression of claudin-1 in the duodenum (n=7 CTL, n=5 AGE-rich; unpaired two-tailed t-test, P=0.6547). f, mRNA expression of claudin-5 in the duodenum (n=7 CTL, n=7 AGE-rich; unpaired two-tailed t-test, P=0.0111). g, mRNA expression of mucin 2 in the duodenum (n=8 CTL, n=7 AGE-rich; unpaired two-tailed t-test, P=0.5817). h, mRNA expression of occludin in the jejunum (n=5 CTL, n=7 AGE-rich; unpaired two-tailed t-test, P=0.1992). i, mRNA expression of claudin-1 in the jejunum (n=5 CTL, n=7 AGE-rich; unpaired two-tailed t-test, P=0.1429). j, mRNA expression of claudin-5 in the jejunum (n=5 CTL, n=7 AGE-rich; unpaired two-tailed t-test, P=0.2541). k, mRNA expression of mucin 2 in the jejunum (n=5 CTL, n=8 AGE-rich; unpaired two-tailed t-test, P=0.1327). Error bars represent standard error of the mean (SEM). *P<0.05. All n’s indicate number of rats per group. Additional details about the statistical analyses for each subpanel can be found in . AGE, advanced glycation end-product; CTL, control [diet]; mRNA, messenger RNA; NFĸB, nuclear factor kappa-light-chain-enhancer of activated B cells; PN, postnatal day; PSD95, postsynaptic density protein-95; RAGE, receptor for advanced glycation end-products.

Article Snippet: The following Applied Biosystems probes for use with rats were used: C3 (Rn00566466_m1), C5 (Rn01436156_m1), C3aR (Rn00583199_m1), C5aR1 (Rn02134203_s1), RAGE (Rn01525753_g1), NfĸB (Rn01502266_m1), PSD-95 (Rn00571479_m1), occludin (Rn00580064_m1), claudin-1 (Rn00581740_m1), claudin-5 (Rn01753146_s1), and mucin 2 (Rn01498206_m1). qPCR was performed using Applied Biosystems TaqMan Fast Advanced Master Mix (Cat. no. 444557, ThermoFisher Scientific) and an Applied Biosystems QuantStudio 5 Real-Time PCR System (ThermoFisher Scientific).

Techniques: Expressing, Two Tailed Test, Control

Journal: Experimental and Therapeutic Medicine

Article Title: Appendiceal mucinous tumour resulting in autoamputation of the appendix: A case report and literature review

doi: 10.3892/etm.2025.13043

Figure Lengend Snippet: Immunohistochemical markers in the removed appendix tissue. (A) CKpan positivity; (B) CK7 negativity; (C) CK20 positivity; (D) caudal type homeobox 2 positivity; (E) MUC2 positivity; (F) MUC5AC negativity; (G) PAX-2 negativity; (H) PAX-8 negativity; and (I) Ki-67 negativity. CK, cytokeratin; PAX, paired box; MUC, mucin.

Article Snippet: Primary antibody details were as follows: CK-pan mouse monoclonal antibody (7H8C4; 1:200; cat. no. EM1712-42; HUABIO), CK7 recombinant monoclonal antibody (1:2,000; cat. no. 86153-7-RR; Proteintech Group, Inc.), CK20 recombinant monoclonal antibody (1:4,000; cat. no. 82428-1-RR; Proteintech Group, Inc.), CDX2 recombinant monoclonal antibody (1:1,000; cat. no. 82659-1-RR; Proteintech Group, Inc.), MUC2 polyclonal antibody (1:2,000; cat. no. 27675-1-AP; Proteintech Group, Inc.), MUC5AC polyclonal antibody (1:500; cat. no. 20725-1-AP; Proteintech Group, Inc.), PAX2 polyclonal antibody (1:1,000; cat. no. 29307-1-AP; Proteintech Group, Inc.), PAX8 polyclonal antibody (1:800; cat. no. 10336-1-AP; Proteintech Group, Inc.) and Ki-67 polyclonal antibody (1:4,000; cat. no. 27309-1-AP; Proteintech Group, Inc.).

Techniques: Immunohistochemical staining

Journal: Drug Metabolism and Disposition

Article Title: Canine duodenal organoids as a functional platform for intestinal CYP regulation and drug metabolism studies

doi: 10.1016/j.dmd.2025.100191

Figure Lengend Snippet: Expression of differentiation markers and CYP genes in duodenal organoids under differentiation conditions. Five differentiation markers, alkaline phosphatase ( ALP ), CgA , leucine-rich repeat-containing G-protein coupled receptor 5 ( Lgr5 ), lysozyme ( Lyz ), and mucin 2 ( MUC2 ), as well as 2 CYP genes, CYP2B11 and CYP3A98 mRNA, were quantified using RT-qPCR. Two biological replicates (dog 1 and dog 3) were used for this analysis, and differentiation/nondifferentiation treatment was replicated 3 times. The error bars represent the SD. ∗ P < .05; ∗∗ P < .01. NS, not significant.

Article Snippet: We quantified a variety of lineage-specific marker genes, including alkaline phosphatase for epithelial cells, CgA for enteroendocrine cells, leucine-rich repeat-containing G-protein coupled receptor 5 ( Lgr5 ) for stem cells, lysozyme ( Lyz ) for Paneth cells, and mucin 2 ( MUC2 ) for goblet cells, as well as drug-responsive genes ( CYP2B11 , CYP3A98 , CAR , and PXR ), using RT-qPCR on days 4, 6, and 8 with SYBR Green (Thermo Fisher Scientific) and a CFX96 system (Bio-Rad) following established protocols., , , , , , , GAPDH , HMBS , and SDHA served as reference genes.

Techniques: Expressing, Quantitative RT-PCR