Journal: Bioactive Materials

Article Title: Translational selenium nanoparticles trigger apoptosis in triple-negative breast cancer cells through the MAPKs/Bcl2 pathway

doi: 10.1016/j.bioactmat.2026.02.027

Figure Lengend Snippet: Schematic illustration of PTR-SeNPs and MUC1@PTR-SeNPs synthesis and their anti-tumor efficacy against human triple-negative breast cancer.

Article Snippet: Anti-human MUC1 therapeutic antibody Fab fragment (7B8) were purchased From Creative Biolabs (TAB-423MZ-F, USA).

Techniques:

Journal: Bioactive Materials

Article Title: Translational selenium nanoparticles trigger apoptosis in triple-negative breast cancer cells through the MAPKs/Bcl2 pathway

doi: 10.1016/j.bioactmat.2026.02.027

Figure Lengend Snippet: Structure characterization of PTR-SeNPs and MUC1@ PTR-SeNPs. Structure characterization of PTR-SeNPs by (A) TEM, (B) Zetasizer Nano ZS, (C, D) Nanosight NS300, (E1-4) HRTEM-EDS and (F, G) FT-IR. (H) Confirmation of MUC1-C + PTR-SeNPs conjugation by confocal microscopy after fluorescent labeling with anti-mouse IgG (H + L). (I, J) Characterization results of the particle size and potential of MUC1@PTR-SeNPs

Article Snippet: Anti-human MUC1 therapeutic antibody Fab fragment (7B8) were purchased From Creative Biolabs (TAB-423MZ-F, USA).

Techniques: Conjugation Assay, Confocal Microscopy, Labeling

Journal: Bioactive Materials

Article Title: Translational selenium nanoparticles trigger apoptosis in triple-negative breast cancer cells through the MAPKs/Bcl2 pathway

doi: 10.1016/j.bioactmat.2026.02.027

Figure Lengend Snippet: In vitro anti-tumor efficacy of PTR-SeNPs and MUC1@PTR-SeNPs on 17 TNBC c ell lines. ( A, B ) Protein expression level of MUC1 in 17 different TNBC cell lines. ( C, D ) IC 50 and maximum % growth inhibition of PTR-SeNPs and MUC1@PTR-SeNPs on 17 TNBC cell lines. ( E, G ) Cell cycle distribution triggered by PTR-SeNPs and MUC1@PTR-SeNPs in HCC1937 and MDA-MB-436 cells. After treatment with PTR-SeNPs or MUC1@PTR-SeNPs (4 and 40 μM) in HCC1937 and MDA-MB-436 cells for 72 h, cells were stained with propidium iodide followed by flow cytometry analysis using MultiCycle software. The apoptotic cell death was quantified by measuring the sub-G1 cell population. ( F, H ) Phosphatidylserine translocation mediated by PTR-SeNPs and MUC1@PTR-SeNPs in HCC1937 and MDA-MB-436 cells. After treatment with MUC1@PTR-SeNPs (4 and 40 μM) for 48 h, cells were co-stained with propidium iodide and Annexin-V-FITC followed by flow cytometry analysis [early apoptotic subset: Annexin V+/PI- (green); late apoptotic subset: Annexin V+/PT+ (red)].

Article Snippet: Anti-human MUC1 therapeutic antibody Fab fragment (7B8) were purchased From Creative Biolabs (TAB-423MZ-F, USA).

Techniques: In Vitro, Expressing, Inhibition, Staining, Flow Cytometry, Software, Translocation Assay

Journal: Bioactive Materials

Article Title: Translational selenium nanoparticles trigger apoptosis in triple-negative breast cancer cells through the MAPKs/Bcl2 pathway

doi: 10.1016/j.bioactmat.2026.02.027

Figure Lengend Snippet: In vivo anti-tumor efficacy of MUC1@PTR- SeNPs. (A) MUC1 mRNA expression in normal tissue and primary breast cancer tumor using GEPIA database. ( B ) MUC1 expression in tumor tissues of MDA-MB-468-bearing mice in preliminary study. (C – E) Dose-dependent study of tumor inhibition effect of MUC1@PTR-SeNPs [75 (Low), 375 (Mid) & 750 μg (High) Se/kg BW/day] on BALB/c nude mice transplanted with MDA-MB-468 xenograft after oral administration for 30 days. PTR-SeNPs (High; 750 μg Se/kg BW/day) was used to investigate the possible improvement of in vivo anti-tumor efficacy by the MUC1@PTR-SeNPs. Quantitative analysis of Se content (μg/g) in (F) blood and (G) tumor tissue of experimental mice. (H) H&E, Ki67 and Tunnel fluorescence staining of tumor sections to detect apoptosis in vivo . (I) Western blot analysis of PARP, p-Bcl-2, Bax and C-caspase-9 protein expression in tumor sections. (J) In the serum of each group of tumor-bearing mice, the results of blood biochemistry-related indexes were analyzed.

Article Snippet: Anti-human MUC1 therapeutic antibody Fab fragment (7B8) were purchased From Creative Biolabs (TAB-423MZ-F, USA).

Techniques: In Vivo, Expressing, Inhibition, Fluorescence, Staining, Western Blot

Journal: Cells

Article Title: MUC1 Protects Preimplantation Embryos In Vitro via Clearance of ROS by Triggering Mitophagy

doi: 10.3390/cells15090806

Figure Lengend Snippet: Expression and localization of MUC1 in gametes and preimplantation embryos. ( A – D ) Representative immunofluorescence images stained with antibody against MUC1 (green) and co-stained with DAPI (to stain DNA, blue) in human oocyte, mouse oocyte, human sperm, and mouse sperm, respectively. Magnified immunofluorescence image of human sperm is presented within the white box in ( C ). ( E ) Representative images stained with antibody against MUC1 (red) and co-stained with DAPI (to stain DNA, blue) from the 2PN stage to the blastocyst stage. NCtrl, negative control (no primary antibody). Scale bars: 20 µm. ( F ) Quantification of MUC1 fluorescence intensity using ImageJ. Number of samples analyzed at each stage: 2PN = 23, 2CELL = 25, 4CELL = 23, 8CELL = 23, MORULA = 19, and BLASTO = 29 (analyzed using ANOVA). ( G ) Real-time quantitative PCR results for Muc1 mRNA expression levels during early mouse embryonic development. Relative expression level of Muc1 is presented as fold change normalized to β-ACTIN. Number of samples analyzed at each stage: 2PN = 20, 2CELL = 16, 4CELL = 23, 8CELL = 17, MORULA = 17, and BLASTO = 15 (analyzed using ANOVA). Data in ( F , G ) are means ± SDs from at least three independent experiments.

Article Snippet: Wild-type and Muc1 knockout mice, both on the C57BL/6J background, were generated by the Shanghai Model Organisms Center, Inc. (Shanghai, China), as previously described [ ].

Techniques: Expressing, Immunofluorescence, Staining, Negative Control, Fluorescence, Real-time Polymerase Chain Reaction

Journal: Cells

Article Title: MUC1 Protects Preimplantation Embryos In Vitro via Clearance of ROS by Triggering Mitophagy

doi: 10.3390/cells15090806

Figure Lengend Snippet: Muc1 knockout has little, if any, effect on fertility in mice. ( A ) Genotyping of wild-type and Muc1 knockout mice. The black arrows indicate the target bands. ( B ) RT-qPCR analysis of wild-type and Muc1 knockout ovarian tissues. Relative expression level of Muc1 is presented as fold change normalized to β-ACTIN (analyzed using two-tailed Student’s t -test). ( C ) Representative immunofluorescence images depicting MUC1 expression in MII oocytes of wild-type and Muc1 knockout mice. MII, metaphase II. Scale bar: 20 µm. ( D ) Quantification of relative MUC1 fluorescence intensity in MII oocytes of wild-type and Muc1 knockout mice. Number of samples: wild-type = 33 and Muc1 knockout = 28 (analyzed using two-tailed Student’s t -test). ( E ) Litter size of wild-type and Muc1 knockout pairs (analyzed using two-tailed Student’s t -test). ( F ) Survival rate of pups of wild-type and Muc1 knockout pairs (analyzed using two-tailed Student’s t -test). ( G ) Offspring sex ratio from wild-type and Muc1 knockout pairs (analyzed using Chi-square test). ( H ) Interpregnancy interval of wild-type and Muc1 knockout pairs for same parents ( left ) and parturition ( right ) (analyzed using two-tailed Student’s t -test). Data in ( B , D ) are means ± SDs from at least three independent experiments. ns: not significant.

Article Snippet: Wild-type and Muc1 knockout mice, both on the C57BL/6J background, were generated by the Shanghai Model Organisms Center, Inc. (Shanghai, China), as previously described [ ].

Techniques: Knock-Out, Quantitative RT-PCR, Expressing, Two Tailed Test, Immunofluorescence, Fluorescence

Journal: Cells

Article Title: MUC1 Protects Preimplantation Embryos In Vitro via Clearance of ROS by Triggering Mitophagy

doi: 10.3390/cells15090806

Figure Lengend Snippet: Muc1 knockout impairs in vitro embryonic development in mice. ( A ) Overview of the experimental design (created in BioRender; https://BioRender.com/2uu2xma (accessed on 25th February 2026)). ( B ) Morphological comparison of D0.5, D1.5, D2.5, D3.0, D3.5, and D4.5 wild-type and Muc1 knockout embryos. Scale bar: 50 µm. White arrows indicate embryos with developmental arrest. ( C ) The rate of embryos that reached the 4-cell stage in wild-type and Muc1 knockout groups. Number of samples: wild-type = 80 and Muc1 knockout = 69 (analyzed using Chi-square test). ( D ) The rate of embryos that reached the blastocyst stage in wild-type and Muc1 knockout groups. Number of samples: wild-type = 80 and Muc1 knockout = 69 (analyzed using Chi-square test). ( E ) Representative immunofluorescence images stained with antibodies against Oct4 (green, ICM) and CDX2 (red, TE), and co-stained with DAPI (blue, nuclei). Scale bar: 10 µm. ( F ) The number of cells in blastocysts (ICM, TE, or total) of wild-type and Muc1 knockout groups based on three-dimensional reconstruction. Number of samples: wild-type = 20 and Muc1 knockout = 20 (analyzed using two-tailed Student’s t -test). ( G ) Representative immunofluorescence images stained with antibodies against Oct4 (green) and MUC1 (red), and co-stained with DAPI (blue). Scale bar: 10 µm. ( H ) Relative telomere length of wild-type and Muc1 knockout blastocysts. Number of samples: wild-type = 27 and Muc1 knockout = 27 (analyzed using two-tailed Student’s t -test). Data in ( C , D , F , H ) are means ± SDs from at least three independent experiments.

Article Snippet: Wild-type and Muc1 knockout mice, both on the C57BL/6J background, were generated by the Shanghai Model Organisms Center, Inc. (Shanghai, China), as previously described [ ].

Techniques: Knock-Out, In Vitro, Comparison, Immunofluorescence, Staining, Two Tailed Test

Journal: Cells

Article Title: MUC1 Protects Preimplantation Embryos In Vitro via Clearance of ROS by Triggering Mitophagy

doi: 10.3390/cells15090806

Figure Lengend Snippet: Muc1 knockout leads to accumulation of mtROS and damaged mitochondria. ( A ) Immunofluorescence staining of MitoSOX (red) and DNA (blue) ( left ), and statistical analysis of MitoSOX fluorescence intensity ( right ) in wild-type and Muc1 knockout groups at the blastocyst stage. Scale bar: 10 µm. Number of samples: wild-type = 22 and Muc1 knockout = 20 (analyzed using two-tailed Student’s t -test). ( B ) Immunofluorescence staining of MitoSOX (red) and DNA (blue) ( left ), and statistical analysis of MitoSOX fluorescence intensity ( right ) in wild-type and Muc1 knockout groups at the 4-cell stage. Scale bar: 10 µm. Number of samples: wild-type = 23 and Muc1 knockout = 20 (analyzed using two-tailed Student’s t -test). ( C ) Immunofluorescence staining of JC-1 (mitochondrial membrane potential) ( left ), and statistical analysis of JC-1 (red-to-green ratio) ( right ) in wild-type and Muc1 knockout groups at the blastocyst stage. Scale bar: 10 µm. Number of samples: wild-type = 23 and Muc1 knockout = 21 (analyzed using two-tailed Student’s t -test). ( D ) Immunofluorescence staining of p62 (green) and DNA (blue) ( left ), and statistical analysis of p62 fluorescence intensity ( right ) in wild-type and Muc1 knockout groups at the blastocyst stage. Scale bar: 10 µm. Number of samples: wild-type = 22 and Muc1 knockout = 21 (analyzed using two-tailed Student’s t -test). Data in ( A – D ) are means ± SDs from at least three independent experiments.

Article Snippet: Wild-type and Muc1 knockout mice, both on the C57BL/6J background, were generated by the Shanghai Model Organisms Center, Inc. (Shanghai, China), as previously described [ ].

Techniques: Knock-Out, Immunofluorescence, Staining, Fluorescence, Two Tailed Test, Membrane

Journal: Cells

Article Title: MUC1 Protects Preimplantation Embryos In Vitro via Clearance of ROS by Triggering Mitophagy

doi: 10.3390/cells15090806

Figure Lengend Snippet: Muc1 knockout leads to reduction in mitophagy. ( A ) Immunofluorescence staining of LC3 and MitoTracker in wild-type and Muc1 knockout groups at the blastocyst stage. Blue, DNA; green, LC3; red, MitoTracker. Scale bar: 10 µm. White arrows indicate the co-localization of LC3 and MitoTracker. ( B ) Pearson correlation coefficient for co-localization of LC3 and MitoTracker at the blastocyst stage. Number of samples: wild-type = 28, Muc1 knockout = 27 (analyzed using two-tailed Student’s t -test). ( C ) Pearson correlation coefficient for co-localization of LysoTracker and MitoTracker at the blastocyst stage. Number of samples: wild-type = 22 and Muc1 knockout = 21 (analyzed using two-tailed Student’s t -test). ( D ) Immunofluorescence staining of LysoTracker and MitoTracker in wild-type and Muc1 knockout groups at the blastocyst stage. Blue, DNA; green, MitoTracker; red, LysoTracker. Scale bar: 10 µm. White arrows indicate co-localization of LysoTracker and MitoTracker. ( E ) Immunofluorescence staining of LC3 and MitoTracker in wild-type and Muc1 knockout groups at the 4-cell stage. Blue, DNA; green, LC3; red, MitoTracker. Scale bar: 20 µm. White arrows indicate co-localization of LC3 and MitoTracker. ( F ) Pearson correlation coefficient for co-localization of LC3 and MitoTracker at the 4-cell stage. Number of samples: wild-type = 21, Muc1 knockout = 20 (analyzed using two-tailed Student’s t -test). ( G ) Immunofluorescence staining of PINK1 (red) and DNA (blue) ( left ), and statistical analysis of PINK1 fluorescence intensity ( right ) in wild-type and Muc1 knockout groups at the blastocyst stage. Scale bar: 10 µm. Number of samples: wild-type = 21 and Muc1 knockout = 20 (analyzed using two-tailed Student’s t -test). ( H ) Immunofluorescence staining of Parkin (red) and DNA (blue) ( left ), and statistical analysis of Parkin fluorescence intensity ( right ) in wild-type and Muc1 knockout groups at the blastocyst stage. Scale bar: 10 µm. Number of samples: wild-type = 20 and Muc1 knockout = 20 (analyzed using two-tailed Student’s t -test). Data in ( B , C , F – H ) are means ± SDs from at least three independent experiments.

Article Snippet: Wild-type and Muc1 knockout mice, both on the C57BL/6J background, were generated by the Shanghai Model Organisms Center, Inc. (Shanghai, China), as previously described [ ].

Techniques: Knock-Out, Immunofluorescence, Staining, Two Tailed Test, Fluorescence

Journal: Cells

Article Title: MUC1 Protects Preimplantation Embryos In Vitro via Clearance of ROS by Triggering Mitophagy

doi: 10.3390/cells15090806

Figure Lengend Snippet: Low-dose CCCP treatment rescues impaired mitophagy and blastocyst formation defects caused by Muc1 knockout. ( A ) Morphological comparison of D0.5, D1.5, D3.0, D3.5, and D4.5 WT, KO, WT + CCCP, and KO + CCCP embryos. Scale bar: 50 µm. White arrows indicate embryos with developmental arrest. ( B ) Blastocyst formation rates in WT, KO, WT + CCCP, and KO + CCCP groups. Number of samples analyzed in each group: WT = 37, KO = 45, WT + CCCP = 37, and KO + CCCP = 48 (analyzed using Chi-square test). ( C ) Immunofluorescence staining of MitoSOX in WT, KO, WT + CCCP, and KO + CCCP groups at the blastocyst stage. Blue, DNA; red, MitoSOX. Scale bar: 10 µm. ( D ) Relative fluorescence intensity of MitoSOX at the blastocyst stage. Number of samples analyzed in each group was as follows: WT = 20, KO = 21, WT + CCCP = 20, and KO + CCCP = 20 (analyzed using ANOVA). ( E ) Immunofluorescence staining of LC3 and MitoTracker in WT, KO, WT + CCCP, and KO + CCCP groups at the blastocyst stage. Blue, DNA; green, LC3; red, MitoTracker. Scale bar: 10 µm. White arrows indicate the co-localization of LC3 and MitoTracker. ( F ) Pearson correlation coefficient for co-localization of LC3 and MitoTracker at the blastocyst stage. Number of samples analyzed in each group: WT = 23, KO = 20, WT + CCCP = 22, and KO + CCCP = 20 (analyzed using ANOVA). Data in ( B , D , F ) are means ± SDs from at least three independent experiments. ns: not significant.

Article Snippet: Wild-type and Muc1 knockout mice, both on the C57BL/6J background, were generated by the Shanghai Model Organisms Center, Inc. (Shanghai, China), as previously described [ ].

Techniques: Knock-Out, Comparison, Immunofluorescence, Staining, Fluorescence

Journal: Cells

Article Title: MUC1 Protects Preimplantation Embryos In Vitro via Clearance of ROS by Triggering Mitophagy

doi: 10.3390/cells15090806

Figure Lengend Snippet: Vitamin C treatment restores abnormal embryonic development induced by Muc1 knockout by normalizing mtROS levels. ( A ) Morphological comparison of D0.5, D1.5, and D4.5 WT, KO, WT + VC, and KO + VC embryos. Scale bar: 50 µm. White arrows indicate embryos with developmental arrest. ( B ) Blastocyst formation rates in WT, KO, WT + VC, and KO + VC groups. Number of samples analyzed in each group: WT = 35, KO = 35, WT + VC = 34, and KO + VC = 37 (analyzed using Chi-square test). ( C ) Immunofluorescence staining of MitoSOX in WT, KO, WT + VC, and KO + VC groups at the blastocyst stage. Blue, DNA; red, MitoSOX. Scale bar: 10 µm. ( D ) Relative fluorescence intensity of MitoSOX at the blastocyst stage. Number of samples: WT = 22, KO = 20, WT + VC = 20, and KO + VC = 20 (analyzed using ANOVA). ( E ) Schematic overview of the working model (created in BioRender. https://BioRender.com/z21zhau (accessed on 28th February 2026)). The dashed arrow indicates partial blockade of the process. Embryos cultured in vitro are unavoidably exposed to physical stressors, inducing the generation of mtROS. MUC1 maintains the normal progression of early embryonic development by promoting mitophagy, which suppresses mtROS accumulation. Muc1 depletion impairs trophectoderm development and shortens telomere length. Low-dose CCCP and vitamin C treatment restore embryonic development by enhancing mitophagy and clearing mtROS, respectively. Data in ( B , D ) are means ± SDs from at least three independent experiments. ns: not significant.

Article Snippet: Wild-type and Muc1 knockout mice, both on the C57BL/6J background, were generated by the Shanghai Model Organisms Center, Inc. (Shanghai, China), as previously described [ ].

Techniques: Knock-Out, Comparison, Immunofluorescence, Staining, Fluorescence, Cell Culture, In Vitro

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Knockout of Mucin 1 inhibits the proliferation, migration, and invasion of human MDA-MB-231 cells by blocking autophagy flow

doi: 10.1590/1414-431X2026e15075

Figure Lengend Snippet: Mucin 1 (MUC1) expression in human breast cancer. A , MUC1 expression levels in breast cancer (UALCAN website). B , MUC1 expression levels in different subtypes of breast cancer (UALCAN website). C , MUC1 protein expression in breast cancer (Human Protein Atlas database). D , MUC1 expression in human normal mammary epithelial cells MCF10A, TNBC cells (MDA-MB-231), and TNBC tissues (scale bar=100 μm). Data are reported as means±SE. **P<0.01, ***P<0.001, ****P<0.0001; two-tailed unpaired Student's t -test. BRCA: breast cancer gene; TCGA: The Cancer Genome Atlas; TNBC: triple-negative breast cancer.

Article Snippet: IHC data in the Human Protein Atlas database showed increased MUC1 protein expression in breast cancer tissue, but rarely expressed in normal breast tissue.

Techniques: Expressing, Two Tailed Test

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Knockout of Mucin 1 inhibits the proliferation, migration, and invasion of human MDA-MB-231 cells by blocking autophagy flow

doi: 10.1590/1414-431X2026e15075

Figure Lengend Snippet: Construction and validation the Mucin 1 (MUC1) knockout cell line 231-MUC1-KO. A , Sequencing results after MUC1 knockout were confirmed by the Snapgene software to identify the monoclonal cell line 231-MUC1-KO. B , RT-qPCR for MUC1 in MDA-MB-231 and 231-MUC1-KO cells. C , Western blot for MUC1 in MDA-MB-231 and 231-MUC1-KO cells. Data are reported as means±SE. ****P<0.0001; two-tailed unpaired Student's t -test.

Article Snippet: IHC data in the Human Protein Atlas database showed increased MUC1 protein expression in breast cancer tissue, but rarely expressed in normal breast tissue.

Techniques: Biomarker Discovery, Knock-Out, Sequencing, Software, Quantitative RT-PCR, Western Blot, Two Tailed Test

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Knockout of Mucin 1 inhibits the proliferation, migration, and invasion of human MDA-MB-231 cells by blocking autophagy flow

doi: 10.1590/1414-431X2026e15075

Figure Lengend Snippet: Effect of Mucin 1 (MUC1) knockout on the proliferation, migration, and invasion of MDA-MB-231 cells. A , MDA-MB-231 cell proliferation by EDU assay (scale bar=100 μm). B , MDA-MB-231 cell proliferation by colony formation assay. C , Migration and invasion of MDA-MB-231 cells by transwell assay (scale bar=150 μm). Data are reported as means±SE. **P<0.01, ***P<0.001, ****P<0.0001; two-tailed unpaired Student's t -test.

Article Snippet: IHC data in the Human Protein Atlas database showed increased MUC1 protein expression in breast cancer tissue, but rarely expressed in normal breast tissue.

Techniques: Knock-Out, Migration, EdU Assay, Colony Assay, Transwell Assay, Two Tailed Test

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Knockout of Mucin 1 inhibits the proliferation, migration, and invasion of human MDA-MB-231 cells by blocking autophagy flow

doi: 10.1590/1414-431X2026e15075

Figure Lengend Snippet: Effect of Mucin 1 (MUC1) knockout on autophagy flow in MDA-MB-231 cells. A , Expression of LC3 I, LC3 II, and P62 proteins by western blot. B , Ad-mCherry-GFP-LC3B-labeled LC3B protein assessed the patency of intracellular autophagy flow (scale bar=100 μm). Data are reported as means±SE. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001; two-tailed unpaired Student's t -test. BafA1: Bafilomycin A1; Rapa: Rapamycin.

Article Snippet: IHC data in the Human Protein Atlas database showed increased MUC1 protein expression in breast cancer tissue, but rarely expressed in normal breast tissue.

Techniques: Knock-Out, Expressing, Western Blot, Labeling, Two Tailed Test

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Knockout of Mucin 1 inhibits the proliferation, migration, and invasion of human MDA-MB-231 cells by blocking autophagy flow

doi: 10.1590/1414-431X2026e15075

Figure Lengend Snippet: Effect of Mucin 1 (MUC1) knockout on lysosomes in MDA-MB-231 cells. A , Expression of SNAP29 and STXl7 by western blot. B , Expression of LAMP1 by western blot. C , pH was observed with red fluorescent probe Lysotracker Red (scale bar=100 μm). Data are reported as means±SE. **P<0.01, NS: not significant; two-tailed unpaired Student's t -test.

Article Snippet: IHC data in the Human Protein Atlas database showed increased MUC1 protein expression in breast cancer tissue, but rarely expressed in normal breast tissue.

Techniques: Knock-Out, Expressing, Western Blot, Two Tailed Test

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Knockout of Mucin 1 inhibits the proliferation, migration, and invasion of human MDA-MB-231 cells by blocking autophagy flow

doi: 10.1590/1414-431X2026e15075

Figure Lengend Snippet: Schematic diagram of the Mucin 1 (MUC1) oncogenic mechanism in breast cancer cell line MDA-MB-231. Knockout (KO) of MUC1 inhibits the proliferation, migration, and invasion of MDA-MB-231 cells by regulating the autophagic flow, and the possible mechanism may be related to the blocking of the autophagic flow caused by lysosomal damage.

Article Snippet: IHC data in the Human Protein Atlas database showed increased MUC1 protein expression in breast cancer tissue, but rarely expressed in normal breast tissue.

Techniques: Knock-Out, Migration, Blocking Assay