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Unique scFv (by sequence) that bound α-LTX per number of positive hits per number of clones analyzed in α-LTX ELISA.

Journal: Frontiers in Immunology

Article Title: Human antibodies neutralizing the alpha-latrotoxin of the European black widow

doi: 10.3389/fimmu.2024.1407398

Figure Lengend Snippet: Unique scFv (by sequence) that bound α-LTX per number of positive hits per number of clones analyzed in α-LTX ELISA.

Article Snippet: In brief, 180 µL of 2xYT-GA were inoculated with single colonies bearing the scFv-expressing phagemids and incubated overnight at 37°C, 800 rpm in a MTP shaker (VorTemp 56™ Shaking Incubator, LabNet, Edison, NJ, USA).

Techniques: Sequencing, Clone Assay, Enzyme-linked Immunosorbent Assay