Journal: Journal of Traditional and Complementary Medicine

Article Title: Bushen Huoxue Recipe enhances the immune microenvironment at the maternal-fetal interface via the Hippo signaling pathway in mice with recurrent spontaneous abortion

doi: 10.1016/j.jtcme.2025.02.005

Figure Lengend Snippet: Effect of BSHXR on the Hippo signaling pathway. (A) The expression levels of proteins in the Hippo pathway. (B) Quantification of the protein levels. (p-MST/t-MST: Con vs. RSA, p = 0.0179; RSA vs. CsA, p = 0.8320; RSA vs. BSHXR, p = 0.0450; p-LATS/t-LATS: Con vs. RSA, p = 0.0030; RSA vs. CsA, p = 0.0153; RSA vs. BSHXR, p = 0.0327; p-YAP/t-YAP: Con vs. RSA, p = 0.0131; RSA vs. CsA, p = 0.0432; RSA vs. BSHXR, p = 0.0340; p-TAZ/t-TAZ: Con vs. RSA, p = 0.0045; RSA vs. CsA, p = 0.0437; RSA vs. BSHXR, p = 0.0050). (C) Representative images of immunofluorescence staining for YAP and TAZ in decidual tissues. Scale bar: 20 μm (YAP/TAZ/DCS1). (D) The expression of YAP and TAZ in the nuclear or cytoplasmic fraction. (E) Quantification of the protein levels. (YAP: Con vs. RSA, p = 0.0079; RSA vs. CsA, p = 0.0228; RSA vs. BSHXR, p = 0.0163; TAZ: Con vs. RSA, p = 0.0001; RSA vs. CsA, p = 0.0090; RSA vs. BSHXR, p = 0.0006). The data are expressed as the means ± SEMs. ∗ p < 0.05, ∗∗ p < 0.01 and ∗∗∗ p <0.001, compared with the control group; # p < 0.05, ## p < 0.01 and ### p < 0.001, compared with the RSA group.

Article Snippet: Antibodies against TLR2 (66645-1-Ig), TLR4 (66350-1-Ig), TLR7 (17232-1-AP), and GAPDH (60004-1-Ig) were purchased from Proteintech (Rosemont, IL, USA); antibodies against RORγt (ab207082) and FoxP3 (ab215206) were purchased from Abcam (Cambridge, MA, USA); Alexa Fluor 555 nm (8953) and antibodies against MST (14946), p-MST (49332), LATS (3477S), p-LATS (8654S), YAP (14074S), p-YAP (13008S), TAZ (83669S), and p-TAZ (59971S) were purchased from Cell Signaling Technology (Danvers, MA, USA); and an antibody against Lamin B (A11495) was purchased from ABclonal (Wuhan, China).

Techniques: Expressing, Immunofluorescence, Staining, Control

Journal: International Journal of Molecular Sciences

Article Title: The Chronic Elevated Consumption of Hibiscus sabdariffa Linnaeus Results in Kidney Damage Associated with Excess H 2 S

doi: 10.3390/ijms27052190

Figure Lengend Snippet: Immunohistochemistry analysis of areas marked in the kidney cortex on the expression of the enzymes involved in the H 2 S pathway. Panel ( a ) = CBS, ( b ) = CSE, ( c ) = 3–MST and ( d ) = GCLC. Abbreviations: CBS = cystathionine–β–synthase, CSE = cystathionine–γ–lyase, 3–MST = 3–mercaptopyruvate, GCLC = γ-glutamylcysteine synthetase. Data are means ± SE; n = 7 each group. AU = arbitrary units.

Article Snippet: For Western blot, the antibodies were CBS antibody mouse IgG 1 κ: sc-133154, CSE antibody mouse (F-1): sc-374249, MST-3 antibody mouse (46): sc-135993, and GCLC antibody [EP13475], which were provided by Santa Cruz Biotechnology.

Techniques: Immunohistochemistry, Expressing

Journal: International Journal of Molecular Sciences

Article Title: The Chronic Elevated Consumption of Hibiscus sabdariffa Linnaeus Results in Kidney Damage Associated with Excess H 2 S

doi: 10.3390/ijms27052190

Figure Lengend Snippet: Representative Western blot images that represent the expression of the enzymes involved in the H 2 S synthesis pathway in the three experimental groups. Panel ( a ) = CBS, ( b ) = CSE, ( c ) = 3–MST and ( d ) = GCLC. Abbreviations: CBS = cystathionine–β–synthase, CSE = cystathionine–γ–lyase, 3–MST = 3–mercaptopyruvate, GCLC = γ-glutamylcysteine synthetase. Data are means ± SE; n = 7 each group.

Article Snippet: For Western blot, the antibodies were CBS antibody mouse IgG 1 κ: sc-133154, CSE antibody mouse (F-1): sc-374249, MST-3 antibody mouse (46): sc-135993, and GCLC antibody [EP13475], which were provided by Santa Cruz Biotechnology.

Techniques: Western Blot, Expressing