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( a ) <t>TLR2</t> - Arg753Gln , TLR4 - Asp299Gly , IL6 - 174G/C , and IL10 - 1082G/A polymorphisms identification. TLR2 - Arg753Gln polymorphism: lane 1—pBRHaeIIIDigest DNA molecular marker; lanes 2, 6, and 8—Gln/Gln genotype; lanes 3, 4, 7, and 10—Arg/Arg genotype; and lanes 5 and 9—Arg/Gln genotype. ( b ) TLR4 - Asp299Gly polymorphism: lane 1—pBRHAeIII Digest DNA molecular marker, lanes 2, 6, and 10—Asp/Asp genotype, lanes 3, 4, 5, 7, and 9—Asp/Gly genotype, and lane 8—Gly/Gly genotype; ( c ) IL6 - 174G/C polymorphism: lane 1—pBRHaeIIIDigest DNA molecular marker, lanes 2 and 6—GG genotype, lanes 3, 5, 8, 9, and 10—GC genotype, and lanes 4 and 7—CC genotype. ( d ) IL10 -1082G/A polymorphism: lane 1—pBRHaeIIIDigest DNA molecular marker, lane 2—PCR fragment, lane 3—AA genotype, lanes 4, 5, 7, 8, and 10—GA genotype, and lanes 6 and 9—GG genotype.
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mspi enzyme  (New England Biolabs)
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( a ) <t>TLR2</t> - Arg753Gln , TLR4 - Asp299Gly , IL6 - 174G/C , and IL10 - 1082G/A polymorphisms identification. TLR2 - Arg753Gln polymorphism: lane 1—pBRHaeIIIDigest DNA molecular marker; lanes 2, 6, and 8—Gln/Gln genotype; lanes 3, 4, 7, and 10—Arg/Arg genotype; and lanes 5 and 9—Arg/Gln genotype. ( b ) TLR4 - Asp299Gly polymorphism: lane 1—pBRHAeIII Digest DNA molecular marker, lanes 2, 6, and 10—Asp/Asp genotype, lanes 3, 4, 5, 7, and 9—Asp/Gly genotype, and lane 8—Gly/Gly genotype; ( c ) IL6 - 174G/C polymorphism: lane 1—pBRHaeIIIDigest DNA molecular marker, lanes 2 and 6—GG genotype, lanes 3, 5, 8, 9, and 10—GC genotype, and lanes 4 and 7—CC genotype. ( d ) IL10 -1082G/A polymorphism: lane 1—pBRHaeIIIDigest DNA molecular marker, lane 2—PCR fragment, lane 3—AA genotype, lanes 4, 5, 7, 8, and 10—GA genotype, and lanes 6 and 9—GG genotype.
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dna extracts  (New England Biolabs)
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New England Biolabs dna extracts
( a ) <t>TLR2</t> - Arg753Gln , TLR4 - Asp299Gly , IL6 - 174G/C , and IL10 - 1082G/A polymorphisms identification. TLR2 - Arg753Gln polymorphism: lane 1—pBRHaeIIIDigest DNA molecular marker; lanes 2, 6, and 8—Gln/Gln genotype; lanes 3, 4, 7, and 10—Arg/Arg genotype; and lanes 5 and 9—Arg/Gln genotype. ( b ) TLR4 - Asp299Gly polymorphism: lane 1—pBRHAeIII Digest DNA molecular marker, lanes 2, 6, and 10—Asp/Asp genotype, lanes 3, 4, 5, 7, and 9—Asp/Gly genotype, and lane 8—Gly/Gly genotype; ( c ) IL6 - 174G/C polymorphism: lane 1—pBRHaeIIIDigest DNA molecular marker, lanes 2 and 6—GG genotype, lanes 3, 5, 8, 9, and 10—GC genotype, and lanes 4 and 7—CC genotype. ( d ) IL10 -1082G/A polymorphism: lane 1—pBRHaeIIIDigest DNA molecular marker, lane 2—PCR fragment, lane 3—AA genotype, lanes 4, 5, 7, 8, and 10—GA genotype, and lanes 6 and 9—GG genotype.
Dna Extracts, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( a ) TLR2 - Arg753Gln , TLR4 - Asp299Gly , IL6 - 174G/C , and IL10 - 1082G/A polymorphisms identification. TLR2 - Arg753Gln polymorphism: lane 1—pBRHaeIIIDigest DNA molecular marker; lanes 2, 6, and 8—Gln/Gln genotype; lanes 3, 4, 7, and 10—Arg/Arg genotype; and lanes 5 and 9—Arg/Gln genotype. ( b ) TLR4 - Asp299Gly polymorphism: lane 1—pBRHAeIII Digest DNA molecular marker, lanes 2, 6, and 10—Asp/Asp genotype, lanes 3, 4, 5, 7, and 9—Asp/Gly genotype, and lane 8—Gly/Gly genotype; ( c ) IL6 - 174G/C polymorphism: lane 1—pBRHaeIIIDigest DNA molecular marker, lanes 2 and 6—GG genotype, lanes 3, 5, 8, 9, and 10—GC genotype, and lanes 4 and 7—CC genotype. ( d ) IL10 -1082G/A polymorphism: lane 1—pBRHaeIIIDigest DNA molecular marker, lane 2—PCR fragment, lane 3—AA genotype, lanes 4, 5, 7, 8, and 10—GA genotype, and lanes 6 and 9—GG genotype.

Journal: Life

Article Title: Exploratory Analysis of TLR2, TLR4, Interleukin 6 and Interleukin 10 Gene Polymorphisms in Relation to Clinical Early-Onset Sepsis in Preterm Neonates: A Single-Center Study

doi: 10.3390/life16010103

Figure Lengend Snippet: ( a ) TLR2 - Arg753Gln , TLR4 - Asp299Gly , IL6 - 174G/C , and IL10 - 1082G/A polymorphisms identification. TLR2 - Arg753Gln polymorphism: lane 1—pBRHaeIIIDigest DNA molecular marker; lanes 2, 6, and 8—Gln/Gln genotype; lanes 3, 4, 7, and 10—Arg/Arg genotype; and lanes 5 and 9—Arg/Gln genotype. ( b ) TLR4 - Asp299Gly polymorphism: lane 1—pBRHAeIII Digest DNA molecular marker, lanes 2, 6, and 10—Asp/Asp genotype, lanes 3, 4, 5, 7, and 9—Asp/Gly genotype, and lane 8—Gly/Gly genotype; ( c ) IL6 - 174G/C polymorphism: lane 1—pBRHaeIIIDigest DNA molecular marker, lanes 2 and 6—GG genotype, lanes 3, 5, 8, 9, and 10—GC genotype, and lanes 4 and 7—CC genotype. ( d ) IL10 -1082G/A polymorphism: lane 1—pBRHaeIIIDigest DNA molecular marker, lane 2—PCR fragment, lane 3—AA genotype, lanes 4, 5, 7, 8, and 10—GA genotype, and lanes 6 and 9—GG genotype.

Article Snippet: An amount of 6 μL of amplified DNA was digested with 2 units of MspI (TLR2) , NcoI (TLR4) , LweI (IL6) , XagI (IL10) , and restriction enzymes (New England Biolabs UK, Ltd., Hitchin, UK).

Techniques: Marker, Ubiquitin Proteomics