Journal: ACS Applied Materials & Interfaces

Article Title: Polymer Casting and Water Immersion-Based Large-Area Graphene Transfer for Flexible Electronics Fabrication

doi: 10.1021/acsami.5c23601

Figure Lengend Snippet: Live/Dead cell assay analysis at 7 days on (A) Control hAD-MSCs and (B) hAD-MSCs on patterned graphene/PLA films. DNA damage analysis using a comet assay at 7 days on (C) Control hAD-MSCs and (D) hAD-MSCs on patterned graphene/PLA films. (E) Cell viability analysis of hAD-MSCs on patterned graphene/PLA films and Control at 7 days. (F) Apoptosis analysis on hAD-MSCs on patterned graphene/PLA films at 7 days. Cell cycle analysis on (G) Control hAD-MSCs and (H) hAD-MSCs on patterned graphene/PLA films at 7 days. (I) ICC analysis of stem cell markers CD29 and CD44 for hAD-MSCs growing on patterned graphene/PLA films and Petri dishes (control) at 7 days.

Article Snippet: Human adipose-derived mesenchymal stem cells (hAD-MSCs) obtained from ATCC (PCS-500–011) were cultured in mesenchymal stem cell basal medium (ATCC, PCS-500–030) containing 2.1% of MSC supplement (ATCC, PCS-999–038), 1.2% of l -alanyl- l -glutamine (ATCC, PCS-999–034), and 0.1% of penicillin-streptomycin amphotericin B solution (ATCC, PCS-999–002) in a sterile 75 cm 2 cell culture flask, kept in an incubator at 5% CO 2 and a temperature of 37 °C.

Techniques: Control, Single Cell Gel Electrophoresis, Cell Cycle Assay

Journal: MedComm

Article Title: Enhanced Angiogenic Potential of Electrically Stimulated Human Adipose‐Derived Mesenchymal Stem Cells (MSCs) for Ischemic Tissue Regeneration

doi: 10.1002/mco2.70352

Figure Lengend Snippet: Schematic illustrations of electrically potentiated MSCs (epMSCs) and their improved angiogenic potentials.

Article Snippet: Human adipose‐derived MSCs (hAD‐MSCs) (PromoCell, Heidelberg, Germany) were maintained in MEM‐α (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% antibiotic–antimycotic (100×; Gibco) in an incubator with 5% CO 2 at 37°C used at passage number 6.

Techniques:

Journal: MedComm

Article Title: Enhanced Angiogenic Potential of Electrically Stimulated Human Adipose‐Derived Mesenchymal Stem Cells (MSCs) for Ischemic Tissue Regeneration

doi: 10.1002/mco2.70352

Figure Lengend Snippet: Electrical stimulation (ES) of hAD‐MSCs. (A) Schematic illustrations of in vitro experimental procedures and ES parameters. A constant voltage of 0.3 V with low frequency (LF, 1 Hz) and high frequency (HF, 100 Hz) was used for ES of hAD‐MSCs. (B) LIVE/DEAD staining images of the unstimulated MSCs (control) or MSCs stimulated with LF or HF. Green and red cells represent live and dead cells, respectively. Scale bars = 200 µm. (C) Cell viability of hAD‐MSCs ( n = 4). Cell viability was expressed as the percentage of live cells among the total (live and dead) cells. (D) Relative metabolic activity of unstimulated or electrically stimulated MSCs. Relative metabolic activity of each group was demonstrated as absorbance at 450 nm normalized by that of the unstimulated control ( n = 4).

Article Snippet: Human adipose‐derived MSCs (hAD‐MSCs) (PromoCell, Heidelberg, Germany) were maintained in MEM‐α (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% antibiotic–antimycotic (100×; Gibco) in an incubator with 5% CO 2 at 37°C used at passage number 6.

Techniques: In Vitro, Staining, Control, Activity Assay

Journal: MedComm

Article Title: Enhanced Angiogenic Potential of Electrically Stimulated Human Adipose‐Derived Mesenchymal Stem Cells (MSCs) for Ischemic Tissue Regeneration

doi: 10.1002/mco2.70352

Figure Lengend Snippet: Expression of typical angiogenic markers in epMSCs. (A) Relative mRNA expression of VEGF‐A, HGF, bFGF, and IGF‐1 genes. Expression of each gene was normalized to that of GAPDH ( n = 4). (B) Quantification of VEGF‐A and HGF produced from MSCs and epMSCs ( n = 4). (C) Fold change in the mean pixel density of each growth factor produced by MSCs and epMSCs ( n = 4). CMs from MSCs and epMSCs were analyzed using a human growth factor antibody array to detect growth factor production in MSCs. Gene expression was normalized to GAPDH using the 2 −ΔΔCt method. An asterisk (*) denotes a statistically significant difference ( p < 0.05).

Article Snippet: Human adipose‐derived MSCs (hAD‐MSCs) (PromoCell, Heidelberg, Germany) were maintained in MEM‐α (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% antibiotic–antimycotic (100×; Gibco) in an incubator with 5% CO 2 at 37°C used at passage number 6.

Techniques: Expressing, Produced, Ab Array, Gene Expression

Journal: MedComm

Article Title: Enhanced Angiogenic Potential of Electrically Stimulated Human Adipose‐Derived Mesenchymal Stem Cells (MSCs) for Ischemic Tissue Regeneration

doi: 10.1002/mco2.70352

Figure Lengend Snippet: Quantitative RNA sequencing analysis. (A) Volcano plot of differentially expressed genes (DEGs). The red and blue dots indicate the upregulated and downregulated genes in the epMSCs compared with those in the MSCs, respectively. (B) Hierarchical clustering analysis of DEGs between the MSCs and epSCs with a color scale indicating upregulation (red) and downregulation (blue). ( n = 3 per group). (C) Ten major gene ontology (GO) analysis of the genes of which expressions were significantly influenced by ES. (D) Gene expression profile associated with GO terms related to angiogenesis. Genes depicted in blue and red indicate upregulation and downregulation of angiogenesis, respectively.

Article Snippet: Human adipose‐derived MSCs (hAD‐MSCs) (PromoCell, Heidelberg, Germany) were maintained in MEM‐α (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% antibiotic–antimycotic (100×; Gibco) in an incubator with 5% CO 2 at 37°C used at passage number 6.

Techniques: RNA Sequencing, Gene Expression

Journal: MedComm

Article Title: Enhanced Angiogenic Potential of Electrically Stimulated Human Adipose‐Derived Mesenchymal Stem Cells (MSCs) for Ischemic Tissue Regeneration

doi: 10.1002/mco2.70352

Figure Lengend Snippet: Proteomics analysis of MSCs and epMSCs. (A) Venn diagram illustrating the distribution of proteins identified the CM of MSCs and epMSCs. (B) Hierarchical clustering analysis of the differentially expressed proteins between MSC CM and epMSC CM with a color scale indicating downregulation (blue) and upregulation (red). (C) GO analysis highlighting the top 10 biological processes significantly altered by ES with. (D) Functional enrichment analysis of differentially expressed proteins with the top 10 significantly enriched GO terms related to signaling pathways.

Article Snippet: Human adipose‐derived MSCs (hAD‐MSCs) (PromoCell, Heidelberg, Germany) were maintained in MEM‐α (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% antibiotic–antimycotic (100×; Gibco) in an incubator with 5% CO 2 at 37°C used at passage number 6.

Techniques: Functional Assay, Protein-Protein interactions