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ms2 phage operator stem loop  (Genovis Inc)
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Genovis Inc ms2 phage operator stem loop
Ms2 Phage Operator Stem Loop, supplied by Genovis Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ms2 phage operator stem loop/product/Genovis Inc
Average 99 stars, based on 1 article reviews
ms2 phage operator stem loop - by Bioz Stars, 2026-03
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ms2 stem loops  (Addgene inc)
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(A) Schematic of G3BP1 protein domains and mutants used. (B and C) Rose plots (B) and quantification of speed (μm/min), represented as boxplots, (C) from individually migrating cells from control or G3BP1 shRNA-treated cells with either no G3BP1, full-length (FL), ΔRBD, or ΔNTF2L re-expression (one-way ANOVA with multiple comparisons, p < 0.0001 for Con or FL vs. shRNA alone, ΔRBD, or ΔNTF2L, and p = 0.0445 for ΔRBD vs. ΔNTF2L; n = 247–346 cells per group). (D) Boxplots of FA-G3BP1 correlation coefficient from control or re-expression cells in E (one-way ANOVA with multiple comparisons, p < 0.0001 for G3BP1 shRNA only vs. all other comparisons, for Con shRNA vs. ΔRBD or ΔNTF2L, or FL vs. ΔNTF2L; p = 0.0115 for FL vs. ΔRBD; n = 15 for all groups, but n = 7 for G3BP1 shRNA alone). (E) Representative images of G3BP1 (yellow) and PXN (magenta) in control or G3BP1 shRNA-treated cells with G3BP1 re-expression. Dotted lines represent outlines of proteins. Dotted boxes in the whole-cell image represent the region highly magnified along the bottom below. (F) Schematic of <t>MS2-MCP-GFP-PXN</t> system. TRAK2-MS2-tagged mRNA was co-expressed with an MCP-GFP-PXN fusion protein to directly link RNA to FAs. (G) Representative images of G3BP1 RNP complexes (yellow) and TRAK2-MS2 (cyan) in HDFs co-expressing MCP-GFP-PXN, which is incorporated into the FAs (magenta). Dotted lines represent outlines of protein/mRNA. Dotted boxes in images represent the highly magnified regions below. (H) Quantification of smFISH images taken of cells expressing MCP-GFP-PXN and either MS2 alone or TRAK2-MS2. Boxplots represent the log2 of FA enrichment for mRNA foci directly on top of FA (co-localization) (two-way unpaired t test, p < 0.0001, n = 21 cells). (I) Boxplots of FA-G3BP1 correlation coefficient from MS2-only- or TRAK2-MS2-expressing cells (two-way unpaired t test, p = 0.0472, n = 25 cells). (J and K) Rose plots (microns) (J) and quantification of speed (μm/min), represented as boxplots, (K) from individually migrating cells from control or G3BP1 shRNA-treated cells with either MS2 only or TRAK2-MS2 (one-way ANOVA with multiple comparisons, p < 0.0001 for MS2 only vs. TRAK2-MS2 Con shRNA and MS2 only or TRAK2-MS2 Con shRNA vs. both G3BP1 shRNA groups; n = 498–755 cells/group). See also and , , , , , , , , and .
Ms2 Stem Loops, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ms2 stem loops/product/Addgene inc
Average 93 stars, based on 1 article reviews
ms2 stem loops - by Bioz Stars, 2026-03
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rna stem-loop complexed with the bacteriophage ms2 capsid  (Stonehouse Enterprises LLC)
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Stonehouse Enterprises LLC rna stem-loop complexed with the bacteriophage ms2 capsid
(A) Schematic of G3BP1 protein domains and mutants used. (B and C) Rose plots (B) and quantification of speed (μm/min), represented as boxplots, (C) from individually migrating cells from control or G3BP1 shRNA-treated cells with either no G3BP1, full-length (FL), ΔRBD, or ΔNTF2L re-expression (one-way ANOVA with multiple comparisons, p < 0.0001 for Con or FL vs. shRNA alone, ΔRBD, or ΔNTF2L, and p = 0.0445 for ΔRBD vs. ΔNTF2L; n = 247–346 cells per group). (D) Boxplots of FA-G3BP1 correlation coefficient from control or re-expression cells in E (one-way ANOVA with multiple comparisons, p < 0.0001 for G3BP1 shRNA only vs. all other comparisons, for Con shRNA vs. ΔRBD or ΔNTF2L, or FL vs. ΔNTF2L; p = 0.0115 for FL vs. ΔRBD; n = 15 for all groups, but n = 7 for G3BP1 shRNA alone). (E) Representative images of G3BP1 (yellow) and PXN (magenta) in control or G3BP1 shRNA-treated cells with G3BP1 re-expression. Dotted lines represent outlines of proteins. Dotted boxes in the whole-cell image represent the region highly magnified along the bottom below. (F) Schematic of <t>MS2-MCP-GFP-PXN</t> system. TRAK2-MS2-tagged mRNA was co-expressed with an MCP-GFP-PXN fusion protein to directly link RNA to FAs. (G) Representative images of G3BP1 RNP complexes (yellow) and TRAK2-MS2 (cyan) in HDFs co-expressing MCP-GFP-PXN, which is incorporated into the FAs (magenta). Dotted lines represent outlines of protein/mRNA. Dotted boxes in images represent the highly magnified regions below. (H) Quantification of smFISH images taken of cells expressing MCP-GFP-PXN and either MS2 alone or TRAK2-MS2. Boxplots represent the log2 of FA enrichment for mRNA foci directly on top of FA (co-localization) (two-way unpaired t test, p < 0.0001, n = 21 cells). (I) Boxplots of FA-G3BP1 correlation coefficient from MS2-only- or TRAK2-MS2-expressing cells (two-way unpaired t test, p = 0.0472, n = 25 cells). (J and K) Rose plots (microns) (J) and quantification of speed (μm/min), represented as boxplots, (K) from individually migrating cells from control or G3BP1 shRNA-treated cells with either MS2 only or TRAK2-MS2 (one-way ANOVA with multiple comparisons, p < 0.0001 for MS2 only vs. TRAK2-MS2 Con shRNA and MS2 only or TRAK2-MS2 Con shRNA vs. both G3BP1 shRNA groups; n = 498–755 cells/group). See also and , , , , , , , , and .
Rna Stem Loop Complexed With The Bacteriophage Ms2 Capsid, supplied by Stonehouse Enterprises LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rna stem-loop complexed with the bacteriophage ms2 capsid/product/Stonehouse Enterprises LLC
Average 90 stars, based on 1 article reviews
rna stem-loop complexed with the bacteriophage ms2 capsid - by Bioz Stars, 2026-03
90/100 stars
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ms2 stem loop modified grna backbone for cjcas9  (Addgene inc)
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Addgene inc ms2 stem loop modified grna backbone for cjcas9
(A) Schematic of G3BP1 protein domains and mutants used. (B and C) Rose plots (B) and quantification of speed (μm/min), represented as boxplots, (C) from individually migrating cells from control or G3BP1 shRNA-treated cells with either no G3BP1, full-length (FL), ΔRBD, or ΔNTF2L re-expression (one-way ANOVA with multiple comparisons, p < 0.0001 for Con or FL vs. shRNA alone, ΔRBD, or ΔNTF2L, and p = 0.0445 for ΔRBD vs. ΔNTF2L; n = 247–346 cells per group). (D) Boxplots of FA-G3BP1 correlation coefficient from control or re-expression cells in E (one-way ANOVA with multiple comparisons, p < 0.0001 for G3BP1 shRNA only vs. all other comparisons, for Con shRNA vs. ΔRBD or ΔNTF2L, or FL vs. ΔNTF2L; p = 0.0115 for FL vs. ΔRBD; n = 15 for all groups, but n = 7 for G3BP1 shRNA alone). (E) Representative images of G3BP1 (yellow) and PXN (magenta) in control or G3BP1 shRNA-treated cells with G3BP1 re-expression. Dotted lines represent outlines of proteins. Dotted boxes in the whole-cell image represent the region highly magnified along the bottom below. (F) Schematic of <t>MS2-MCP-GFP-PXN</t> system. TRAK2-MS2-tagged mRNA was co-expressed with an MCP-GFP-PXN fusion protein to directly link RNA to FAs. (G) Representative images of G3BP1 RNP complexes (yellow) and TRAK2-MS2 (cyan) in HDFs co-expressing MCP-GFP-PXN, which is incorporated into the FAs (magenta). Dotted lines represent outlines of protein/mRNA. Dotted boxes in images represent the highly magnified regions below. (H) Quantification of smFISH images taken of cells expressing MCP-GFP-PXN and either MS2 alone or TRAK2-MS2. Boxplots represent the log2 of FA enrichment for mRNA foci directly on top of FA (co-localization) (two-way unpaired t test, p < 0.0001, n = 21 cells). (I) Boxplots of FA-G3BP1 correlation coefficient from MS2-only- or TRAK2-MS2-expressing cells (two-way unpaired t test, p = 0.0472, n = 25 cells). (J and K) Rose plots (microns) (J) and quantification of speed (μm/min), represented as boxplots, (K) from individually migrating cells from control or G3BP1 shRNA-treated cells with either MS2 only or TRAK2-MS2 (one-way ANOVA with multiple comparisons, p < 0.0001 for MS2 only vs. TRAK2-MS2 Con shRNA and MS2 only or TRAK2-MS2 Con shRNA vs. both G3BP1 shRNA groups; n = 498–755 cells/group). See also and , , , , , , , , and .
Ms2 Stem Loop Modified Grna Backbone For Cjcas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ms2 stem loop modified grna backbone for cjcas9/product/Addgene inc
Average 90 stars, based on 1 article reviews
ms2 stem loop modified grna backbone for cjcas9 - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


(A) Schematic of G3BP1 protein domains and mutants used. (B and C) Rose plots (B) and quantification of speed (μm/min), represented as boxplots, (C) from individually migrating cells from control or G3BP1 shRNA-treated cells with either no G3BP1, full-length (FL), ΔRBD, or ΔNTF2L re-expression (one-way ANOVA with multiple comparisons, p < 0.0001 for Con or FL vs. shRNA alone, ΔRBD, or ΔNTF2L, and p = 0.0445 for ΔRBD vs. ΔNTF2L; n = 247–346 cells per group). (D) Boxplots of FA-G3BP1 correlation coefficient from control or re-expression cells in E (one-way ANOVA with multiple comparisons, p < 0.0001 for G3BP1 shRNA only vs. all other comparisons, for Con shRNA vs. ΔRBD or ΔNTF2L, or FL vs. ΔNTF2L; p = 0.0115 for FL vs. ΔRBD; n = 15 for all groups, but n = 7 for G3BP1 shRNA alone). (E) Representative images of G3BP1 (yellow) and PXN (magenta) in control or G3BP1 shRNA-treated cells with G3BP1 re-expression. Dotted lines represent outlines of proteins. Dotted boxes in the whole-cell image represent the region highly magnified along the bottom below. (F) Schematic of MS2-MCP-GFP-PXN system. TRAK2-MS2-tagged mRNA was co-expressed with an MCP-GFP-PXN fusion protein to directly link RNA to FAs. (G) Representative images of G3BP1 RNP complexes (yellow) and TRAK2-MS2 (cyan) in HDFs co-expressing MCP-GFP-PXN, which is incorporated into the FAs (magenta). Dotted lines represent outlines of protein/mRNA. Dotted boxes in images represent the highly magnified regions below. (H) Quantification of smFISH images taken of cells expressing MCP-GFP-PXN and either MS2 alone or TRAK2-MS2. Boxplots represent the log2 of FA enrichment for mRNA foci directly on top of FA (co-localization) (two-way unpaired t test, p < 0.0001, n = 21 cells). (I) Boxplots of FA-G3BP1 correlation coefficient from MS2-only- or TRAK2-MS2-expressing cells (two-way unpaired t test, p = 0.0472, n = 25 cells). (J and K) Rose plots (microns) (J) and quantification of speed (μm/min), represented as boxplots, (K) from individually migrating cells from control or G3BP1 shRNA-treated cells with either MS2 only or TRAK2-MS2 (one-way ANOVA with multiple comparisons, p < 0.0001 for MS2 only vs. TRAK2-MS2 Con shRNA and MS2 only or TRAK2-MS2 Con shRNA vs. both G3BP1 shRNA groups; n = 498–755 cells/group). See also and , , , , , , , , and .

Journal: Cell reports

Article Title: G3BP1 ribonucleoprotein complexes regulate focal adhesion protein mobility and cell migration

doi: 10.1016/j.celrep.2025.115237

Figure Lengend Snippet: (A) Schematic of G3BP1 protein domains and mutants used. (B and C) Rose plots (B) and quantification of speed (μm/min), represented as boxplots, (C) from individually migrating cells from control or G3BP1 shRNA-treated cells with either no G3BP1, full-length (FL), ΔRBD, or ΔNTF2L re-expression (one-way ANOVA with multiple comparisons, p < 0.0001 for Con or FL vs. shRNA alone, ΔRBD, or ΔNTF2L, and p = 0.0445 for ΔRBD vs. ΔNTF2L; n = 247–346 cells per group). (D) Boxplots of FA-G3BP1 correlation coefficient from control or re-expression cells in E (one-way ANOVA with multiple comparisons, p < 0.0001 for G3BP1 shRNA only vs. all other comparisons, for Con shRNA vs. ΔRBD or ΔNTF2L, or FL vs. ΔNTF2L; p = 0.0115 for FL vs. ΔRBD; n = 15 for all groups, but n = 7 for G3BP1 shRNA alone). (E) Representative images of G3BP1 (yellow) and PXN (magenta) in control or G3BP1 shRNA-treated cells with G3BP1 re-expression. Dotted lines represent outlines of proteins. Dotted boxes in the whole-cell image represent the region highly magnified along the bottom below. (F) Schematic of MS2-MCP-GFP-PXN system. TRAK2-MS2-tagged mRNA was co-expressed with an MCP-GFP-PXN fusion protein to directly link RNA to FAs. (G) Representative images of G3BP1 RNP complexes (yellow) and TRAK2-MS2 (cyan) in HDFs co-expressing MCP-GFP-PXN, which is incorporated into the FAs (magenta). Dotted lines represent outlines of protein/mRNA. Dotted boxes in images represent the highly magnified regions below. (H) Quantification of smFISH images taken of cells expressing MCP-GFP-PXN and either MS2 alone or TRAK2-MS2. Boxplots represent the log2 of FA enrichment for mRNA foci directly on top of FA (co-localization) (two-way unpaired t test, p < 0.0001, n = 21 cells). (I) Boxplots of FA-G3BP1 correlation coefficient from MS2-only- or TRAK2-MS2-expressing cells (two-way unpaired t test, p = 0.0472, n = 25 cells). (J and K) Rose plots (microns) (J) and quantification of speed (μm/min), represented as boxplots, (K) from individually migrating cells from control or G3BP1 shRNA-treated cells with either MS2 only or TRAK2-MS2 (one-way ANOVA with multiple comparisons, p < 0.0001 for MS2 only vs. TRAK2-MS2 Con shRNA and MS2 only or TRAK2-MS2 Con shRNA vs. both G3BP1 shRNA groups; n = 498–755 cells/group). See also and , , , , , , , , and .

Article Snippet: MS2 Stem loops were cloned from pET263-pUC57 24xMS2V7 (Addgene, #140705).

Techniques: Control, shRNA, Expressing

Journal: Cell reports

Article Title: G3BP1 ribonucleoprotein complexes regulate focal adhesion protein mobility and cell migration

doi: 10.1016/j.celrep.2025.115237

Figure Lengend Snippet:

Article Snippet: MS2 Stem loops were cloned from pET263-pUC57 24xMS2V7 (Addgene, #140705).

Techniques: Control, Recombinant, SYBR Green Assay, Cell Culture, Protease Inhibitor, Mutagenesis, Isolation, Sequencing, shRNA, Plasmid Preparation, Software