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CD146 expression in <t>MRT</t> <t>cell</t> lines and primary tumors. ( a ) MP-MRT-AN, KP-MRT-NS, KP-MRT-RY and KP-MRT-YM cells were stained with anti-CD146 (black histograms) or isotype-matched control antibodies (gray histograms), and then analyzed by flow cytometry. ( b ) Representative flow cytometric profile of ATRT primary tumor cells stained with anti-CD146 and anti-CD133 antibodies, showing that a small CD146 + sub-population was present, whereas CD133 + cells were rarely observed.
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CD146 expression in <t>MRT</t> <t>cell</t> lines and primary tumors. ( a ) MP-MRT-AN, KP-MRT-NS, KP-MRT-RY and KP-MRT-YM cells were stained with anti-CD146 (black histograms) or isotype-matched control antibodies (gray histograms), and then analyzed by flow cytometry. ( b ) Representative flow cytometric profile of ATRT primary tumor cells stained with anti-CD146 and anti-CD133 antibodies, showing that a small CD146 + sub-population was present, whereas CD133 + cells were rarely observed.
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CD146 expression in <t>MRT</t> <t>cell</t> lines and primary tumors. ( a ) MP-MRT-AN, KP-MRT-NS, KP-MRT-RY and KP-MRT-YM cells were stained with anti-CD146 (black histograms) or isotype-matched control antibodies (gray histograms), and then analyzed by flow cytometry. ( b ) Representative flow cytometric profile of ATRT primary tumor cells stained with anti-CD146 and anti-CD133 antibodies, showing that a small CD146 + sub-population was present, whereas CD133 + cells were rarely observed.
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CD146 expression in <t>MRT</t> <t>cell</t> lines and primary tumors. ( a ) MP-MRT-AN, KP-MRT-NS, KP-MRT-RY and KP-MRT-YM cells were stained with anti-CD146 (black histograms) or isotype-matched control antibodies (gray histograms), and then analyzed by flow cytometry. ( b ) Representative flow cytometric profile of ATRT primary tumor cells stained with anti-CD146 and anti-CD133 antibodies, showing that a small CD146 + sub-population was present, whereas CD133 + cells were rarely observed.
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CD146 expression in <t>MRT</t> <t>cell</t> lines and primary tumors. ( a ) MP-MRT-AN, KP-MRT-NS, KP-MRT-RY and KP-MRT-YM cells were stained with anti-CD146 (black histograms) or isotype-matched control antibodies (gray histograms), and then analyzed by flow cytometry. ( b ) Representative flow cytometric profile of ATRT primary tumor cells stained with anti-CD146 and anti-CD133 antibodies, showing that a small CD146 + sub-population was present, whereas CD133 + cells were rarely observed.
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CD146 expression in MRT cell lines and primary tumors. ( a ) MP-MRT-AN, KP-MRT-NS, KP-MRT-RY and KP-MRT-YM cells were stained with anti-CD146 (black histograms) or isotype-matched control antibodies (gray histograms), and then analyzed by flow cytometry. ( b ) Representative flow cytometric profile of ATRT primary tumor cells stained with anti-CD146 and anti-CD133 antibodies, showing that a small CD146 + sub-population was present, whereas CD133 + cells were rarely observed.

Journal: Oncogene

Article Title: CD146 is a novel marker for highly tumorigenic cells and a potential therapeutic target in malignant rhabdoid tumor

doi: 10.1038/onc.2016.72

Figure Lengend Snippet: CD146 expression in MRT cell lines and primary tumors. ( a ) MP-MRT-AN, KP-MRT-NS, KP-MRT-RY and KP-MRT-YM cells were stained with anti-CD146 (black histograms) or isotype-matched control antibodies (gray histograms), and then analyzed by flow cytometry. ( b ) Representative flow cytometric profile of ATRT primary tumor cells stained with anti-CD146 and anti-CD133 antibodies, showing that a small CD146 + sub-population was present, whereas CD133 + cells were rarely observed.

Article Snippet: MRT cell lines were treated with 0.05% trypsin/EDTA (Life Technologies), and sorted cells were resuspended in serum-free α-minimum essential medium for experiments.

Techniques: Expressing, Staining, Control, Flow Cytometry