Journal: International Journal of Molecular Sciences

Article Title: The Effects of Human BDH2 on the Cell Cycle, Differentiation, and Apoptosis and Associations with Leukemia Transformation in Myelodysplastic Syndrome

doi: 10.3390/ijms21093033

Figure Lengend Snippet: Expression levels of ( A ) BDH2 and ( B ) LCN2 mRNA in BM in MDS and control patients, including de novo CN-AML and normal BM. The expression levels of the BDH2 and LCN2 genes were normalized to the internal control β-actin to obtain the relative threshold cycle (ΔC T ). BDH2, hydroxybutyrate dehydrogenase type 2; BM, bone marrow; CN-AML, cytogenetically normal acute myeloid leukemia; LCN2, lipocalin 2; MDS, myelodysplastic syndrome; RA, refractory anemia; RAEB, refractory anemia with excess blasts; RARS, refractory anemia with ringed sideroblasts.

Article Snippet: In order to find the target genes of BDH2, we used an mRNA microarray service (Phalanx Biotech Group, Inc., Taiwan) on shRNA-BDH2-3 THP1 cells and empty vector transfected THP1 cells.

Techniques: Expressing, Control

Journal: International Journal of Molecular Sciences

Article Title: The Effects of Human BDH2 on the Cell Cycle, Differentiation, and Apoptosis and Associations with Leukemia Transformation in Myelodysplastic Syndrome

doi: 10.3390/ijms21093033

Figure Lengend Snippet: RNA microarray analysis of BDH2 targeting genes. Group 1: genes related to mitochondria; group 2: genes that have been reported as oncogenes or tumor suppressor genes; group 3: genes that function to control the survival, growth, differentiation, proliferation, and effector function of tissues and cells. All of those genes showed statistical significance with p < 0.05.

Article Snippet: In order to find the target genes of BDH2, we used an mRNA microarray service (Phalanx Biotech Group, Inc., Taiwan) on shRNA-BDH2-3 THP1 cells and empty vector transfected THP1 cells.

Techniques: Microarray, Control

Journal: British Journal of Cancer

Article Title: Tumour suppressor microRNA-584 directly targets oncogene Rock-1 and decreases invasion ability in human clear cell renal cell carcinoma

doi: 10.1038/sj.bjc.6606028

Figure Lengend Snippet: ROCK-1 is a miR-584 target gene. ( A ) Messenger RNA microarray data of ROCK-1. The miR-584 or miR control were transfected into A-498 cells. At 72 h after transfection, total RNA was extracted and mRNA expression level was compared by Phalanx Bio Inc. ( B ) Sequence of miR-584/ROCK-1 and 3′UTR luciferase assay. The miR-584 binding site in the ROCK-1 3′UTR predicted with microRNA org. (upper). 3′UTR vector and miR-584 or miR control were co-transfected into A-498 cells. Cell lysates were measured for relative luciferase activities at 48 h after transfection. Levels of luciferase activity were compared with those of miR control-transfected cells that is normalised to 1. ( C ) The ROCK-1 protein levels in RCC cells transfected with miR-584. The miR-584 or miR control was transfected into A-498 and 769-P cells. At 72 h after transfection, total protein was extracted and analysed by western blots. β -Actin was used as a loading control. The ratio of band intensity is relative to that of β -actin. Band intensity was measured by using ImageJ software.

Article Snippet: In order to find potential target oncogenes of tumour suppressive miR-584, we used an mRNA microarray service (Phalanx Bio Inc., Palo Alto, CA, USA).

Techniques: Microarray, Control, Transfection, Expressing, Sequencing, Luciferase, Binding Assay, Plasmid Preparation, Activity Assay, Western Blot, Software

Journal: British Journal of Cancer

Article Title: Tumour suppressor microRNA-584 directly targets oncogene Rock-1 and decreases invasion ability in human clear cell renal cell carcinoma

doi: 10.1038/sj.bjc.6606028

Figure Lengend Snippet: Functional effects of ROCK-1 knockdown on A-498 and 769-P cells. ( A ) Expression of ROCK-1 mRNA. si-ROCK-1 or si-NC were transfected into A-498 and 769-P cells. At 48 h after transfection, ROCK-1 mRNA was analysed using RT-PCR. ROCK-1 expression was normalised to β -actin. ( B ) ROCK-1 protein levels. At 72 h after transfection, total protein was extracted and analysed by western blots. β -actin was used as a loading control. ( C ) Wound healing assay of si-ROCK-1 transfectants. si-ROCK-1or si- NC was transfected into A-498 and 769-P cells. At 48 h after transfection, cells were transferred from 6-well to 12-well plates and further incubated for 24 h. A wound was then formed by scraping and measured after 24 h.

Article Snippet: In order to find potential target oncogenes of tumour suppressive miR-584, we used an mRNA microarray service (Phalanx Bio Inc., Palo Alto, CA, USA).

Techniques: Functional Assay, Knockdown, Expressing, Transfection, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control, Wound Healing Assay, Incubation