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rabbit monoclonal d2l9i  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit monoclonal d2l9i
    Rabbit Monoclonal D2l9i, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mpc1/MPC1+Rabbit+mAb/pmc12989855-5-1-5
    Average 95 stars, based on 103 article reviews
    rabbit monoclonal d2l9i - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Unraveling mitochondrial pyruvate dysfunction to mitigate hyperlactatemia and lethality in sepsis.
    Article Snippet: .. After blocking the membranes with a 1⁄2 dilution of Starting Block/PBST0,1% (Thermo Fisher Scientific), the membranes were overnight incubated at 4 ◦ C with primary antibodies against GPT2 (1:1000; 16757-1-AP, Thermo Fisher Scientific), MPC1 (1:1000; 14462S, Cell Signaling Technology), MPC2 (1:1000; 46141S, Cell Signaling Technology), PCX (1:1000; 66470S, Cell Signaling Technology), PDH (1:1000; 3205S, Cell Signaling Technology), P-PDH Ser232 (1:1000; 1063, Sigma), PPDH Ser293 (1:1000; 31866S, Cell Signaling Technology), P-PDH Ser300 (1:1000; ABS194, Sigma), PDK3 (1:1000; PA5-28019, Thermo Fisher Scientific), PDK4 (1:1000; PA5-13776, Thermo Fisher Scientific), and β-ACTIN (1:5000; MA5-15739, Thermo Fisher Scientific), β-TUBULIN (1:1000; 2146S, Cell Signaling Technology), GAPDH (1:1000; 2118T, Cell Signaling Technology) as loading controls. .. The blots were washed with PBST0.1% and incubated for 1h at room temperature with Amersham ECL anti-mouse antibody (1:2000, GENA931, GE Healthcare Life Sciences) or Amersham ECL anti-rabbit antibody (1:2000; GEN934, GE Healthcare Life Sciences).

    Incubation:

    Article Title: Unraveling mitochondrial pyruvate dysfunction to mitigate hyperlactatemia and lethality in sepsis.
    Article Snippet: .. After blocking the membranes with a 1⁄2 dilution of Starting Block/PBST0,1% (Thermo Fisher Scientific), the membranes were overnight incubated at 4 ◦ C with primary antibodies against GPT2 (1:1000; 16757-1-AP, Thermo Fisher Scientific), MPC1 (1:1000; 14462S, Cell Signaling Technology), MPC2 (1:1000; 46141S, Cell Signaling Technology), PCX (1:1000; 66470S, Cell Signaling Technology), PDH (1:1000; 3205S, Cell Signaling Technology), P-PDH Ser232 (1:1000; 1063, Sigma), PPDH Ser293 (1:1000; 31866S, Cell Signaling Technology), P-PDH Ser300 (1:1000; ABS194, Sigma), PDK3 (1:1000; PA5-28019, Thermo Fisher Scientific), PDK4 (1:1000; PA5-13776, Thermo Fisher Scientific), and β-ACTIN (1:5000; MA5-15739, Thermo Fisher Scientific), β-TUBULIN (1:1000; 2146S, Cell Signaling Technology), GAPDH (1:1000; 2118T, Cell Signaling Technology) as loading controls. .. The blots were washed with PBST0.1% and incubated for 1h at room temperature with Amersham ECL anti-mouse antibody (1:2000, GENA931, GE Healthcare Life Sciences) or Amersham ECL anti-rabbit antibody (1:2000; GEN934, GE Healthcare Life Sciences).

    other:


    Article Title: Metabolic Radiosensitization by Targeting Lactate Metabolism with Microfluidic Liposomal Nanocarriers
    Article Snippet: Primary antibodies against α-tubulin (No. 2144S) and MPC1 (No. 14462S) were obtained from Cell Signaling Technology.

    Article Title: Metabolic Radiosensitization by Targeting Lactate Metabolism with Microfluidic Liposomal Nanocarriers.
    Article Snippet: Primary antibodies against α-tubulin (No. 2144S) and MPC1 (No. 14462S) were obtained from Cell Signaling Technology.



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    mpc1 antibody  (Cell Signaling Technology Inc)
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    (A) Western blot image of <t>MPC1</t> at 12 kD in the soluble fraction from 37 to 66°C of whole cells treated with vehicle control and UK5099. (B) The western blot was quantified using Image Lab.Relative intensities were used to quantify protein abundance normalized to 37°C. Curves fitted to the Boltzmann Sigmoid equation and demonstrate no statistically significant difference between the melting temperature of MPC1 in the control and UK5099-treated conditions (n = 1, N = 3, extra sum of squares F test, p = 0.91). (C) Western blot image of MPC at 12 kD in the soluble fraction of whole cell lysate treated with vehicle control and UK5099. (D) Quantified protein abundance in the soluble fraction fitted to the Boltzmann Sigmoid equation demonstrate no significant difference in the melting temperatures between control and UK5099-treated conditions (n = 1, N = 3, extra sum of squares F test, p = 0.82). Blot images are representative of means obtained from independent replicates.
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    Image Search Results


    (A) Western blot image of MPC1 at 12 kD in the soluble fraction from 37 to 66°C of whole cells treated with vehicle control and UK5099. (B) The western blot was quantified using Image Lab.Relative intensities were used to quantify protein abundance normalized to 37°C. Curves fitted to the Boltzmann Sigmoid equation and demonstrate no statistically significant difference between the melting temperature of MPC1 in the control and UK5099-treated conditions (n = 1, N = 3, extra sum of squares F test, p = 0.91). (C) Western blot image of MPC at 12 kD in the soluble fraction of whole cell lysate treated with vehicle control and UK5099. (D) Quantified protein abundance in the soluble fraction fitted to the Boltzmann Sigmoid equation demonstrate no significant difference in the melting temperatures between control and UK5099-treated conditions (n = 1, N = 3, extra sum of squares F test, p = 0.82). Blot images are representative of means obtained from independent replicates.

    Journal: bioRxiv

    Article Title: Cellular thermal shift assay of subcellular isolates for evaluating drug-membrane target interactions

    doi: 10.64898/2026.02.03.703651

    Figure Lengend Snippet: (A) Western blot image of MPC1 at 12 kD in the soluble fraction from 37 to 66°C of whole cells treated with vehicle control and UK5099. (B) The western blot was quantified using Image Lab.Relative intensities were used to quantify protein abundance normalized to 37°C. Curves fitted to the Boltzmann Sigmoid equation and demonstrate no statistically significant difference between the melting temperature of MPC1 in the control and UK5099-treated conditions (n = 1, N = 3, extra sum of squares F test, p = 0.91). (C) Western blot image of MPC at 12 kD in the soluble fraction of whole cell lysate treated with vehicle control and UK5099. (D) Quantified protein abundance in the soluble fraction fitted to the Boltzmann Sigmoid equation demonstrate no significant difference in the melting temperatures between control and UK5099-treated conditions (n = 1, N = 3, extra sum of squares F test, p = 0.82). Blot images are representative of means obtained from independent replicates.

    Article Snippet: The membrane was blocked in 2% BSA for two days before being probed with 1:500 MPC1 antibody (Cell Signaling Ref 14462) [ ], 1:2000 MPC2 antibody (Cell Signaling Ref 46141) [ ], 1:2000 Porin VDAC antibody (Calbio Ref 529534), or 1:2000 ß-actin antibody (Cell Signaling Ref 3700) overnight.

    Techniques: Western Blot, Control, Quantitative Proteomics

    (A) Western blot image of MPC1 in the soluble fraction between the temperatures of 37 and 66°C in DMSO and UK5099-treated conditions. (B) The western blot was quantified as described above. The DMSO samples were fitted to a Boltzmann Sigmoid curve and compared to the UK5099 samples (n = 1-2, N = 3, paired t-test assuming unequal variance, * = p < 0.05, ** = p < 0.01). (C) Western blot image of MPC2 in the soluble fraction between the temperatures of 37 and 66°C in DMSO and UK5099-treated conditions. (D) The western blots were quantified as above and the DMSO samples were fitted to a Boltzmann Sigmoid curve and compared to the UK5099 samples (n = 1-2, N = 3, extra sum of squares F-test, p < 0.05). Blot images are representative of means obtained from independent replicates.

    Journal: bioRxiv

    Article Title: Cellular thermal shift assay of subcellular isolates for evaluating drug-membrane target interactions

    doi: 10.64898/2026.02.03.703651

    Figure Lengend Snippet: (A) Western blot image of MPC1 in the soluble fraction between the temperatures of 37 and 66°C in DMSO and UK5099-treated conditions. (B) The western blot was quantified as described above. The DMSO samples were fitted to a Boltzmann Sigmoid curve and compared to the UK5099 samples (n = 1-2, N = 3, paired t-test assuming unequal variance, * = p < 0.05, ** = p < 0.01). (C) Western blot image of MPC2 in the soluble fraction between the temperatures of 37 and 66°C in DMSO and UK5099-treated conditions. (D) The western blots were quantified as above and the DMSO samples were fitted to a Boltzmann Sigmoid curve and compared to the UK5099 samples (n = 1-2, N = 3, extra sum of squares F-test, p < 0.05). Blot images are representative of means obtained from independent replicates.

    Article Snippet: The membrane was blocked in 2% BSA for two days before being probed with 1:500 MPC1 antibody (Cell Signaling Ref 14462) [ ], 1:2000 MPC2 antibody (Cell Signaling Ref 46141) [ ], 1:2000 Porin VDAC antibody (Calbio Ref 529534), or 1:2000 ß-actin antibody (Cell Signaling Ref 3700) overnight.

    Techniques: Western Blot

    (A) Schematic representing hypothesis that cellular lysis disrupts mitochondrial membrane integrity whereas with use of mitochondrial isolation it is preserved. (B) Western blot image of MPC1 in the soluble fraction between the temperatures 37 and 66°C in control versus UK5099 treated samples. (C) The samples were fitted to the Boltzmann Sigmoid curve, and no significant difference was observed in the MPC1 melting temperature between control and UK5099-treated conditions (n = 1-2, N = 3, extra sum of square F test, p = 0.7611). Blot images are representative of means obtained from independent replicates.

    Journal: bioRxiv

    Article Title: Cellular thermal shift assay of subcellular isolates for evaluating drug-membrane target interactions

    doi: 10.64898/2026.02.03.703651

    Figure Lengend Snippet: (A) Schematic representing hypothesis that cellular lysis disrupts mitochondrial membrane integrity whereas with use of mitochondrial isolation it is preserved. (B) Western blot image of MPC1 in the soluble fraction between the temperatures 37 and 66°C in control versus UK5099 treated samples. (C) The samples were fitted to the Boltzmann Sigmoid curve, and no significant difference was observed in the MPC1 melting temperature between control and UK5099-treated conditions (n = 1-2, N = 3, extra sum of square F test, p = 0.7611). Blot images are representative of means obtained from independent replicates.

    Article Snippet: The membrane was blocked in 2% BSA for two days before being probed with 1:500 MPC1 antibody (Cell Signaling Ref 14462) [ ], 1:2000 MPC2 antibody (Cell Signaling Ref 46141) [ ], 1:2000 Porin VDAC antibody (Calbio Ref 529534), or 1:2000 ß-actin antibody (Cell Signaling Ref 3700) overnight.

    Techniques: Lysis, Membrane, Isolation, Western Blot, Control