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qRT-PCR primers used.

Journal: Journal of Immunology Research

Article Title: Identification of Differentially Expressed lncRNAs in a CpG ODN-Activated Macrophage

doi: 10.1155/2020/1407654

Figure Lengend Snippet: qRT-PCR primers used.

Article Snippet: The CapitalBio Technology mouse lncRNA microarray v4.0 was applied for the profiling analysis of mouse lncRNAs and mRNAs.

Techniques:

Changed expression profiles of lncRNAs and mRNAs during CpG ODN-induced macrophage activation. (a) The heat map of all lncRNA expression at different time points during CpG ODN-induced macrophage activation from microarray data. (b) Scatter plots showing differentially expressed lncRNAs between macrophages with the stimulation with CpG ODN at 0 h and activated macrophages at different time points (4 h, 8 h, and 16 h). (c) Often differentially expressed lncRNAs between macrophages with the stimulation with CpG ODN at 0 h and activated macrophages at different time points (4 h, 8 h, and 16 h). (d) Hierarchical clustering showing often upregulated and downregulated lncRNAs among the four groups (macrophages stimulated for 0 h, 4 h, 8 h, and 16 h). (e) The heat map of all mRNA expression at different time points during CpG ODN-induced macrophage activation from microarray data. (f) Scatter plots showing differentially expressed mRNAs between macrophages with the stimulation with CpG ODN at 0 h and activated macrophages at different time points (4 h, 8 h, and 16 h). (g) Often differentially expressed mRNAs between macrophages with the stimulation with CpG ODN at 0 h and activated macrophages at different time points (4 h, 8 h, and 16 h). (h) Hierarchical clustering showing often up- and downregulated mRNAs among the four groups (macrophages stimulated for 0 h, 4 h, 8 h, and 16 h).

Journal: Journal of Immunology Research

Article Title: Identification of Differentially Expressed lncRNAs in a CpG ODN-Activated Macrophage

doi: 10.1155/2020/1407654

Figure Lengend Snippet: Changed expression profiles of lncRNAs and mRNAs during CpG ODN-induced macrophage activation. (a) The heat map of all lncRNA expression at different time points during CpG ODN-induced macrophage activation from microarray data. (b) Scatter plots showing differentially expressed lncRNAs between macrophages with the stimulation with CpG ODN at 0 h and activated macrophages at different time points (4 h, 8 h, and 16 h). (c) Often differentially expressed lncRNAs between macrophages with the stimulation with CpG ODN at 0 h and activated macrophages at different time points (4 h, 8 h, and 16 h). (d) Hierarchical clustering showing often upregulated and downregulated lncRNAs among the four groups (macrophages stimulated for 0 h, 4 h, 8 h, and 16 h). (e) The heat map of all mRNA expression at different time points during CpG ODN-induced macrophage activation from microarray data. (f) Scatter plots showing differentially expressed mRNAs between macrophages with the stimulation with CpG ODN at 0 h and activated macrophages at different time points (4 h, 8 h, and 16 h). (g) Often differentially expressed mRNAs between macrophages with the stimulation with CpG ODN at 0 h and activated macrophages at different time points (4 h, 8 h, and 16 h). (h) Hierarchical clustering showing often up- and downregulated mRNAs among the four groups (macrophages stimulated for 0 h, 4 h, 8 h, and 16 h).

Article Snippet: The CapitalBio Technology mouse lncRNA microarray v4.0 was applied for the profiling analysis of mouse lncRNAs and mRNAs.

Techniques: Expressing, Activation Assay, Microarray