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ATCC aml12 mouse hepatocytes
Inosine alleviates palmitic acid (PA)-induced damage in <t>AML12</t> cells. (A,B) Cell viability. (C) The effect of inosine (100 μM) intervention on Bodipy staining in PA-induced AML12 cells. (D) mtROS was detected and quantified by MtSOX in vitro , Scale bar = 200 μm. (E) The effect of inosine (100 μM) intervention on JC-1 staining in PA-induced AML12 cells. (F) The relative expression of genes in lipid accumulation, pro-inflammatory factors, and autophagy. Data are expressed as mean ± SD ( n = 4). $ p < 0.05, $$ p < 0.01 compared with NC group. # p < 0.05, ## p < 0.01 compared with PA group.
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mouse hepatocyte aml12 cells  (ATCC)
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Inosine alleviates palmitic acid (PA)-induced damage in <t>AML12</t> cells. (A,B) Cell viability. (C) The effect of inosine (100 μM) intervention on Bodipy staining in PA-induced AML12 cells. (D) mtROS was detected and quantified by MtSOX in vitro , Scale bar = 200 μm. (E) The effect of inosine (100 μM) intervention on JC-1 staining in PA-induced AML12 cells. (F) The relative expression of genes in lipid accumulation, pro-inflammatory factors, and autophagy. Data are expressed as mean ± SD ( n = 4). $ p < 0.05, $$ p < 0.01 compared with NC group. # p < 0.05, ## p < 0.01 compared with PA group.
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mouse hepatocyte aml12 cells - by Bioz Stars, 2026-08
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aml 12 mouse hepatocytes  (ATCC)
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ATCC aml 12 mouse hepatocytes
Inosine alleviates palmitic acid (PA)-induced damage in <t>AML12</t> cells. (A,B) Cell viability. (C) The effect of inosine (100 μM) intervention on Bodipy staining in PA-induced AML12 cells. (D) mtROS was detected and quantified by MtSOX in vitro , Scale bar = 200 μm. (E) The effect of inosine (100 μM) intervention on JC-1 staining in PA-induced AML12 cells. (F) The relative expression of genes in lipid accumulation, pro-inflammatory factors, and autophagy. Data are expressed as mean ± SD ( n = 4). $ p < 0.05, $$ p < 0.01 compared with NC group. # p < 0.05, ## p < 0.01 compared with PA group.
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aml 12 mouse hepatocytes - by Bioz Stars, 2026-08
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transfection mouse hepatocyte aml12 cells  (ATCC)
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ATCC transfection mouse hepatocyte aml12 cells
Inosine alleviates palmitic acid (PA)-induced damage in <t>AML12</t> cells. (A,B) Cell viability. (C) The effect of inosine (100 μM) intervention on Bodipy staining in PA-induced AML12 cells. (D) mtROS was detected and quantified by MtSOX in vitro , Scale bar = 200 μm. (E) The effect of inosine (100 μM) intervention on JC-1 staining in PA-induced AML12 cells. (F) The relative expression of genes in lipid accumulation, pro-inflammatory factors, and autophagy. Data are expressed as mean ± SD ( n = 4). $ p < 0.05, $$ p < 0.01 compared with NC group. # p < 0.05, ## p < 0.01 compared with PA group.
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transfection mouse hepatocyte aml12 cells - by Bioz Stars, 2026-08
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Inosine alleviates palmitic acid (PA)-induced damage in AML12 cells. (A,B) Cell viability. (C) The effect of inosine (100 μM) intervention on Bodipy staining in PA-induced AML12 cells. (D) mtROS was detected and quantified by MtSOX in vitro , Scale bar = 200 μm. (E) The effect of inosine (100 μM) intervention on JC-1 staining in PA-induced AML12 cells. (F) The relative expression of genes in lipid accumulation, pro-inflammatory factors, and autophagy. Data are expressed as mean ± SD ( n = 4). $ p < 0.05, $$ p < 0.01 compared with NC group. # p < 0.05, ## p < 0.01 compared with PA group.

Journal: Frontiers in Nutrition

Article Title: Raspberry aqueous extract ameliorates MAFLD in mice by regulating gut microbiota and purine metabolism

doi: 10.3389/fnut.2026.1818086

Figure Lengend Snippet: Inosine alleviates palmitic acid (PA)-induced damage in AML12 cells. (A,B) Cell viability. (C) The effect of inosine (100 μM) intervention on Bodipy staining in PA-induced AML12 cells. (D) mtROS was detected and quantified by MtSOX in vitro , Scale bar = 200 μm. (E) The effect of inosine (100 μM) intervention on JC-1 staining in PA-induced AML12 cells. (F) The relative expression of genes in lipid accumulation, pro-inflammatory factors, and autophagy. Data are expressed as mean ± SD ( n = 4). $ p < 0.05, $$ p < 0.01 compared with NC group. # p < 0.05, ## p < 0.01 compared with PA group.

Article Snippet: AML12 mouse hepatocytes (ATCC) were cultured in DMEM/F12 (319-085-CL, Wisent, China) supplemented with 10% FBS (12003C, Sigma-Aldrich, United States), 1 × ITS (10 μg/mL insulin, 5 μg/mL transferrin, 5 ng/mL selenium; I3146, Sigma-Aldrich, United States), 40 ng/mL dexamethasone (D4902, Sigma-Aldrich, United States), and 1% streptomycin/penicillin/gentamicin (BL141A, Biosharp, China).

Techniques: Staining, In Vitro, Expressing