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Data are presented as observed vs. Bliss-expected normalized protein concentrations (mean ± SEM). Statistical significance of Δ values was assessed by one-sample t -tests (see ). ATF6 showed strong synergy in both cell lines. IRE1α exhibited slight, non-significant synergy in both CRL-1739 and HT29. GRP78 responses were cell line–dependent, with strong synergy in CRL-1739 but no significant difference (moderate antagonism) in HT29 ( p ≥ 0.05).

Journal: Current Issues in Molecular Biology

Article Title: GPX4 Inhibition Enhances the Pro-Oxidant and ER Stress Effects of Tempol in Colon and Gastric Cancer Cell Lines

doi: 10.3390/cimb47100856

Figure Lengend Snippet: Data are presented as observed vs. Bliss-expected normalized protein concentrations (mean ± SEM). Statistical significance of Δ values was assessed by one-sample t -tests (see ). ATF6 showed strong synergy in both cell lines. IRE1α exhibited slight, non-significant synergy in both CRL-1739 and HT29. GRP78 responses were cell line–dependent, with strong synergy in CRL-1739 but no significant difference (moderate antagonism) in HT29 ( p ≥ 0.05).

Article Snippet: Specifically, IRE1α (catalog no. NBP3–49699), GRP78 (catalog no. NBP2–62145), and ATF6 (catalog no. NBP2–69885) ELISA kits (Novus Biologicals, Centennial, CO, USA) were employed according to the manufacturer’s protocols.

Techniques: