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Establishment and validation of Fbxw7 knockdown (FKD) in Huh-7 cells. A Immunofluorescence staining demonstrated a marked reduction of Fbxw7 expression in FKD cells ( b ) compared with mock control cells ( a ). Nuclei were counterstained with DAPI. B Western blot analysis confirmed decreased Fbxw7 protein levels in FKD cells, validating the successful establishment of stable Fbxw7-deficient Huh-7 cell lines

Journal: Medical Molecular Morphology

Article Title: Loss of Fbxw7 disrupts lipid homeostasis and autophagy in hepatocellular carcinoma cells

doi: 10.1007/s00795-026-00457-3

Figure Lengend Snippet: Establishment and validation of Fbxw7 knockdown (FKD) in Huh-7 cells. A Immunofluorescence staining demonstrated a marked reduction of Fbxw7 expression in FKD cells ( b ) compared with mock control cells ( a ). Nuclei were counterstained with DAPI. B Western blot analysis confirmed decreased Fbxw7 protein levels in FKD cells, validating the successful establishment of stable Fbxw7-deficient Huh-7 cell lines

Article Snippet: Control (mock) Huh‐7 cells were generated by transfecting the empty pRS vector (TR20003; OriGene) without shRNA.

Techniques: Biomarker Discovery, Knockdown, Immunofluorescence, Staining, Expressing, Control, Western Blot