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mNGS workflow in clinical application. CNS, Central nervous system.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Application of metagenomic next-generation sequencing in the diagnosis of infectious diseases

doi: 10.3389/fcimb.2024.1458316

Figure Lengend Snippet: mNGS workflow in clinical application. CNS, Central nervous system.

Article Snippet: The most commonly used mNGS sequencing platform is the Illumina platform, which is based on the core principle of sequencing by synthesis (SBS), which consists of four main steps: DNA library construction, BALF fluid Flowcell adsorption, bridge polymerase chain reaction (PCR) amplification, and SBS ( ).

Techniques:

The comparison between mNGS and tNGS.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Application of metagenomic next-generation sequencing in the diagnosis of infectious diseases

doi: 10.3389/fcimb.2024.1458316

Figure Lengend Snippet: The comparison between mNGS and tNGS.

Article Snippet: The most commonly used mNGS sequencing platform is the Illumina platform, which is based on the core principle of sequencing by synthesis (SBS), which consists of four main steps: DNA library construction, BALF fluid Flowcell adsorption, bridge polymerase chain reaction (PCR) amplification, and SBS ( ).

Techniques: Comparison, Extraction, Next-Generation Sequencing, Sequencing, Mutagenesis, Bacteria

Comparison of testing methods for diagnosing infectious diseases.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Application of metagenomic next-generation sequencing in the diagnosis of infectious diseases

doi: 10.3389/fcimb.2024.1458316

Figure Lengend Snippet: Comparison of testing methods for diagnosing infectious diseases.

Article Snippet: The most commonly used mNGS sequencing platform is the Illumina platform, which is based on the core principle of sequencing by synthesis (SBS), which consists of four main steps: DNA library construction, BALF fluid Flowcell adsorption, bridge polymerase chain reaction (PCR) amplification, and SBS ( ).

Techniques: Comparison, Microscopy, Staining, Mass Spectrometry, Real-time Polymerase Chain Reaction, Multiplex Assay, Sequencing, Multiplexing, Quantitation Assay, Amplification, Bacteria, Generated, Functional Assay, Binding Assay