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In vitro assays confirming functional, definitive erythroid characteristics of the terminally differentiated iRBCs. A) Cytospins combined with benzidine‐giemsa staining on filter‐purified RETs and on nERYs. B) RET‐size measurement by flow cytometry on CD235 + /DRAQ5 ‐ cell population. C) Marker expression pattern of the iRBCs using flow cytometry, n ≥ 58. D) Quantification of Hbe (α2ε2), HbF (α2γ2) and HbA (α2β2) based on HPLC tracks performed on iRBCs, n = 8. E) Oxygen dissociation curve of iRBC lines measured by the Hemox analyzer. F) Deformability assay or RETs and nERY measured by ARCA. G) Blood group phenotype measured on iPSC lines by DNA‐based <t>(MLPA</t> and SNP array)‐ and on iRBCs by protein‐based (flow cytometry)‐ assays, n ≥ 3.
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In vitro assays confirming functional, definitive erythroid characteristics of the terminally differentiated iRBCs. A) Cytospins combined with benzidine‐giemsa staining on filter‐purified RETs and on nERYs. B) RET‐size measurement by flow cytometry on CD235 + /DRAQ5 ‐ cell population. C) Marker expression pattern of the iRBCs using flow cytometry, n ≥ 58. D) Quantification of Hbe (α2ε2), HbF (α2γ2) and HbA (α2β2) based on HPLC tracks performed on iRBCs, n = 8. E) Oxygen dissociation curve of iRBC lines measured by the Hemox analyzer. F) Deformability assay or RETs and nERY measured by ARCA. G) Blood group phenotype measured on iPSC lines by DNA‐based <t>(MLPA</t> and SNP array)‐ and on iRBCs by protein‐based (flow cytometry)‐ assays, n ≥ 3.
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In vitro assays confirming functional, definitive erythroid characteristics of the terminally differentiated iRBCs. A) Cytospins combined with benzidine‐giemsa staining on filter‐purified RETs and on nERYs. B) RET‐size measurement by flow cytometry on CD235 + /DRAQ5 ‐ cell population. C) Marker expression pattern of the iRBCs using flow cytometry, n ≥ 58. D) Quantification of Hbe (α2ε2), HbF (α2γ2) and HbA (α2β2) based on HPLC tracks performed on iRBCs, n = 8. E) Oxygen dissociation curve of iRBC lines measured by the Hemox analyzer. F) Deformability assay or RETs and nERY measured by ARCA. G) Blood group phenotype measured on iPSC lines by DNA‐based <t>(MLPA</t> and SNP array)‐ and on iRBCs by protein‐based (flow cytometry)‐ assays, n ≥ 3.
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In vitro assays confirming functional, definitive erythroid characteristics of the terminally differentiated iRBCs. A) Cytospins combined with benzidine‐giemsa staining on filter‐purified RETs and on nERYs. B) RET‐size measurement by flow cytometry on CD235 + /DRAQ5 ‐ cell population. C) Marker expression pattern of the iRBCs using flow cytometry, n ≥ 58. D) Quantification of Hbe (α2ε2), HbF (α2γ2) and HbA (α2β2) based on HPLC tracks performed on iRBCs, n = 8. E) Oxygen dissociation curve of iRBC lines measured by the Hemox analyzer. F) Deformability assay or RETs and nERY measured by ARCA. G) Blood group phenotype measured on iPSC lines by DNA‐based <t>(MLPA</t> and SNP array)‐ and on iRBCs by protein‐based (flow cytometry)‐ assays, n ≥ 3.
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In vitro assays confirming functional, definitive erythroid characteristics of the terminally differentiated iRBCs. A) Cytospins combined with benzidine‐giemsa staining on filter‐purified RETs and on nERYs. B) RET‐size measurement by flow cytometry on CD235 + /DRAQ5 ‐ cell population. C) Marker expression pattern of the iRBCs using flow cytometry, n ≥ 58. D) Quantification of Hbe (α2ε2), HbF (α2γ2) and HbA (α2β2) based on HPLC tracks performed on iRBCs, n = 8. E) Oxygen dissociation curve of iRBC lines measured by the Hemox analyzer. F) Deformability assay or RETs and nERY measured by ARCA. G) Blood group phenotype measured on iPSC lines by DNA‐based <t>(MLPA</t> and SNP array)‐ and on iRBCs by protein‐based (flow cytometry)‐ assays, n ≥ 3.
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In vitro assays confirming functional, definitive erythroid characteristics of the terminally differentiated iRBCs. A) Cytospins combined with benzidine‐giemsa staining on filter‐purified RETs and on nERYs. B) RET‐size measurement by flow cytometry on CD235 + /DRAQ5 ‐ cell population. C) Marker expression pattern of the iRBCs using flow cytometry, n ≥ 58. D) Quantification of Hbe (α2ε2), HbF (α2γ2) and HbA (α2β2) based on HPLC tracks performed on iRBCs, n = 8. E) Oxygen dissociation curve of iRBC lines measured by the Hemox analyzer. F) Deformability assay or RETs and nERY measured by ARCA. G) Blood group phenotype measured on iPSC lines by DNA‐based (MLPA and SNP array)‐ and on iRBCs by protein‐based (flow cytometry)‐ assays, n ≥ 3.

Journal: Advanced Science

Article Title: Large‐Scale Production of Transfusion‐Ready Red Blood Cells From Induced Pluripotent Stem Cells

doi: 10.1002/advs.202504725

Figure Lengend Snippet: In vitro assays confirming functional, definitive erythroid characteristics of the terminally differentiated iRBCs. A) Cytospins combined with benzidine‐giemsa staining on filter‐purified RETs and on nERYs. B) RET‐size measurement by flow cytometry on CD235 + /DRAQ5 ‐ cell population. C) Marker expression pattern of the iRBCs using flow cytometry, n ≥ 58. D) Quantification of Hbe (α2ε2), HbF (α2γ2) and HbA (α2β2) based on HPLC tracks performed on iRBCs, n = 8. E) Oxygen dissociation curve of iRBC lines measured by the Hemox analyzer. F) Deformability assay or RETs and nERY measured by ARCA. G) Blood group phenotype measured on iPSC lines by DNA‐based (MLPA and SNP array)‐ and on iRBCs by protein‐based (flow cytometry)‐ assays, n ≥ 3.

Article Snippet: The multiplex ligation‐dependent probe amplification (MLPA) and SNP array were carried out by the Sanquin Diagnostic Department (Amsterdam, The Netherlands).

Techniques: In Vitro, Functional Assay, Staining, Purification, Flow Cytometry, Marker, Expressing