Journal: Oncology Reports

Article Title: Inhibitory role of angiopoietin-like 4 for cancer progression in oropharyngeal squamous cell carcinoma

doi: 10.3892/or.2026.9122

Figure Lengend Snippet: Fluorescence intensities of anti-angiopoietin-like 4 (red), anti-Ki-67 (green), and Hoechst (blue) for the FaDu cells. (A-C) The fluorescence intensities of the FaDu cells transfected with angiopoietin-like 4 were significantly different from those of the control. *P<0.05.

Article Snippet: The primers and probes were procured from Applied Biosystems (TaqMan ® Gene Expression Assays) and had the following IDs: ANGPTL4 (Hs01101127_m1), ACTB (Hs01060665_g1), MKI67 (Hs04260396_g1), BAX (Hs0018269_m1), BCL2 (Hs00608023_m1), and CASP3 (Hs00234387_m1).

Techniques: Fluorescence, Transfection, Control

Journal: Oncology Reports

Article Title: Inhibitory role of angiopoietin-like 4 for cancer progression in oropharyngeal squamous cell carcinoma

doi: 10.3892/or.2026.9122

Figure Lengend Snippet: Relative gene expression levels of various mRNAs in the FaDu cells transfected with angiopoietin-like 4 small interfering RNA were determined by reverse transcription-quantitative PCR. The angiopoietin-like 4 level decreased by 38%, and MKI67 expression increased significantly. The expression of BAX decreased, and that of BCL2 increased. CASP3 expression also increased in the angiopoietin-like 4 knockdown cells.

Article Snippet: The primers and probes were procured from Applied Biosystems (TaqMan ® Gene Expression Assays) and had the following IDs: ANGPTL4 (Hs01101127_m1), ACTB (Hs01060665_g1), MKI67 (Hs04260396_g1), BAX (Hs0018269_m1), BCL2 (Hs00608023_m1), and CASP3 (Hs00234387_m1).

Techniques: Gene Expression, Transfection, Small Interfering RNA, Reverse Transcription, Real-time Polymerase Chain Reaction, Expressing, Knockdown

Journal: eBioMedicine

Article Title: Migrasome-mediated clearance of excess PLK4 defines a targetable vulnerability

doi: 10.1016/j.ebiom.2026.106237

Figure Lengend Snippet: Targeting TSPAN6 suppresses tumour growth and metastasis. (A) Real-time cell proliferation assay of PLK4−OE cells treated with scramble or shTSPAN6 RNA. Data are presented as mean values ± SD. (B) Cystal violet staining of shTSPAN6 or control PLK4−OE cells after 14 days of culture. Colony number of each group was quantified on the right. Data are presented as the mean ± SD from three independent repeats. p values were calculated using unpaired two-tailed Student's t-tests, with p < 0.05 considered statistically significant. (C) Fluorescence staining for active apoptosis in shTSPAN6 or control PLK4−OE cells detected by GreenNuc Caspase-3 Assay Kit. The green fluorescence in nuclei indicate active apoptosis. Scale bar, 20 μm. (D) Western blot analysis of cleaved caspase3 in PLK4−OE cells treated with scramble or shTSPAN6 RNA. β-actin was used as the loading control. (E) Western blot analysis of PLK4 protein levels in primary tumour cells derived from 18 patients with triple-negative breast cancer (TNBC). β-Actin served as the loading control (left). Relative PLK4 protein levels were quantified as the ratio of sample to NC (sample/NC). Cases with sample/NC > 1 were classified as PLK4 high (red dots), whereas cases with sample/NC < 1 were classified as PLK4 low (blue dots) (right). (F) Growth curve of tumours in PDX-models treated with scramble or shTSPAN6 RNA. Tumour volumes are presented as mean values ± SD (n = 30 mice per group). p values were calculated by two-way ANOVA. p < 0.05 was considered statistically significant. (G, H) Immunohistochemical staining of Ki67 and cleaved-caspase3 in tumours from PDX-mice treated with scramble or shTSPAN6 RNA. Scale bar, 100 μm. (I) Immunofluorescent staining for PLK4, TSPAN6, and α-tubulin in frozen tumour sections from PLK4 high tumour mice treated with scramble or shTSPAN6 RNA. White arrowheads indicate PLK4 particles colocalized with TSPAN6 in the extracellular space. Yellow arrowheads indicate bipolar spindles in the control group and multipolar spindles in the shTSPAN6 RNA-treated group. Scale bar, 20 μm for each main image and 2 μm for the zoom in section. Regions marked by the arrowheads are shown at higher magnification in the right three columns. (J) Quantification of PLK4-containing particles in the extracellular space of frozen tumour sections from PLK4 high tumour mice treated with scramble or shTSPAN6 RNA. 20 fields of view from tumour sections of three mice per group were analysed. Data are presented as mean ± SD. p values were calculated using unpaired two-tailed Student's t-tests. p < 0.05 was considered statistically significant. (K) Proportion of bipolar spindles in mitotic tumour cells in frozen tumour sections from PLK4 high tumour mice treated with scramble or shTSPAN6 RNA. (L) Western blot analysis of intracellular PLK4 protein levels in PLK4 high tumours derived from PDX-mice treated with scramble or shTSPAN6 RNA. β-Actin was used as the loading control. Quantification is presented as mean ± SD from three independent biological replicates. p values were calculated using unpaired two-tailed Student's t-tests, with p < 0.05 considered statistically significant. (M) Liver and lung tissues were harvested from PDX-mice with or without detectable metastatic lesions. Arrowheads indicate representative metastatic nodules within the organs. (N) Metastatic incidence in the liver and lung tissues of PDX-mice treated with scramble or shTSPAN6 RNA. The number of mice analysed in each group is indicated above the corresponding bar. (O) Metastatic regions in liver and lung tissues visualised by H&E staining. Representative metastatic foci are indicated by arrowheads, with corresponding higher-magnification images shown on the right. Scale bar, 100 μm for each main image and 20 μm for the zoom in section. (P) Quantification of metastatic nodules in the lungs and livers of PDX-mice treated with scramble or shTSPAN6 RNA. Each dot represents an individual mouse bearing metastatic nodules in the indicated organ.

Article Snippet: Primary antibodies were obtained as follows: anti-PLK4 (Cat# MABC544, RRID: AB_2893410 ) and anti-Centrin (Cat# 04–1624, RRID: AB_10563501 ) from Millipore; anti-LAMP1 (Cat# ab25630, RRID: AB_470708 ), LC3B (Cat# ab192890, RRID: AB_2827794 ), Proteasome 20S alpha + beta (Cat# ab22673, RRID: AB_2268907 ), and LAMP2A (Cat# ab18528, RRID: AB_775981 ) from Abcam; anti-ERp72 (Cat# 66365-1-Ig, RRID: AB_2881745 ), GM130 (Cat# 11308-1-AP, RRID: AB_2115327 ), LMAN2 (Cat# 11496-1-AP, RRID: AB_3085375 ), TMED10 (Cat# 15199-1-AP, RRID: AB_2204321 ), ITGB1 (Cat# 12594-1-AP, RRID: AB_2130085 ), and TSPAN6 (Cat# 12293-1-AP, RRID: AB_2213446 ) from Proteintech; anti-cleaved caspase-3 (Cat# 9664, RRID: AB_2070042 ), PDI (Cat# 2446, RRID: AB_2298935 ), and CYCS (Cat# 4272, RRID: AB_2090454 ) from Cell Signalling Technology; anti-HSC70 (Cat# NB120-2788, RRID: AB_2120309 ) and Ki67 (Cat# NB500-170, RRID: AB_10001977 ) from Novus; anti-PIGK (Cat# PA5-28337, RRID: AB_2545813 ), EOGT (Cat# MA5-53414, RRID: AB_3247885 ), TSPAN4 (Cat# PA5-69344, RRID: AB_2688603 ) and Calnexin (Cat# PA5-34754, RRID: AB_2552106 ) from Thermo Fisher Scientific.

Techniques: Proliferation Assay, Staining, Control, Two Tailed Test, Fluorescence, Caspase-3 Assay, Western Blot, Derivative Assay, Immunohistochemical staining

Journal: bioRxiv

Article Title: Comparison studies between Cesium-137 and X-ray irradiators in epithelial injury using in vitro and in vivo models

doi: 10.64898/2026.04.17.719248

Figure Lengend Snippet: RT-qPCR analysis of ( a ) Cdkn1a expression levels after 6Gy, ( b ) Cdkn1a expression levels after 8Gy, ( c ) Mki67 expression levels after 6Gy, ( d ) Mki67 expression levels after 8Gy, ( e ) Lgr5 expression levels after 6Gy, ( f ) Lgr5 expression levels after 8Gy, ( g ) Olfm4 expression levels after 6Gy, ( h ) Olfm4 expression levels after 8Gy, ( i ) Alpi1 expression levels after 6Gy, ( j ) Alpi1 expression levels after 8Gy, ( k ) Chga expression levels after 6Gy, and ( l ) Chga expression levels after 8Gy were calculated as a fold change at 6, 24, 48 and 72 h with either 137 Cs or X-ray irradiation. Actb was used as a housekeeping gene (control). Data points represent the average of three independent experiments, with the mean ±SD indicated. Significance was determined by the student’s test followed by an analysis of the normal distribution (Tukey’s test), *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: Commercially available TaqMan primers detecting mouse ChgA (Mm00514341-FAM), Alpi1 (Mm01285814-FAM), Olfm4 (Mm01320260-FAM), Cdkn1a (Mm00432448-FAM), Lgr5 (Mm00438890-FAM), Mki67 (Mm01278617-FAM), Actb (Mm04394036-VIC) and human CDKN1A (Hs00355782-FAM), KLF4 (Hs00358836-FAM), KLF5 (Hs0000000-FAM), BCL2 (Hs01048932-FAM), BAX (Hs00180269-FAM) and HPRT1 (Hs02800695-VIC) transcripts were used.

Techniques: Quantitative RT-PCR, Expressing, Irradiation, Control

Journal: bioRxiv

Article Title: Comparison studies between Cesium-137 and X-ray irradiators in epithelial injury using in vitro and in vivo models

doi: 10.64898/2026.04.17.719248

Figure Lengend Snippet: Representative images of immunofluorescence staining ( a ) for Bmi1 YFP -positive cells marker (YFP), proliferation marker (MKi67), and nuclei marker (DAPI) in the duodenum of Bmi1-Cre ER mice exposed to 0 or 12 Gy TBI at 96 h with 137 Cs or X-ray irradiation or 12Gy X-ray ABD irradiation. Scale bar = 50 µm. Quantification of proliferating crypts ( b ) of the duodenum from mice exposed to 0 or 12 Gy 137 Cs or X-ray TBI or 12Gy X-ray ABD irradiation at 96 h. Analysis was performed on sham (100 crypts) and irradiated (300 crypts) mice. Quantification of MKi67-positive and DAPI-positive crypts of the duodenum from mice exposed to 0 or 12 Gy 137 Cs or X-ray TBI or 12Gy X-ray ABD irradiation at 96 h. Data points represent the average of three ( b ) or five ( c ) independent experiments with the mean ±SD indicated. Significance was determined by the Student’s test followed by an analysis of the normal distribution (Tukey’s test), *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: Commercially available TaqMan primers detecting mouse ChgA (Mm00514341-FAM), Alpi1 (Mm01285814-FAM), Olfm4 (Mm01320260-FAM), Cdkn1a (Mm00432448-FAM), Lgr5 (Mm00438890-FAM), Mki67 (Mm01278617-FAM), Actb (Mm04394036-VIC) and human CDKN1A (Hs00355782-FAM), KLF4 (Hs00358836-FAM), KLF5 (Hs0000000-FAM), BCL2 (Hs01048932-FAM), BAX (Hs00180269-FAM) and HPRT1 (Hs02800695-VIC) transcripts were used.

Techniques: Immunofluorescence, Staining, Marker, Irradiation

Journal: CytoJournal

Article Title: Development and validation of a multiparameter prognostic model for extranodal natural killer/T-cell lymphoma: Integration of clinical, pathological, and molecular biomarkers

doi: 10.25259/Cytojournal_34_2025

Figure Lengend Snippet: Graphical Representation of Survival Probabilities of QDB - related Indicators and Diagnostic Performance of NIPI (a) The threshold value of MTP53 by QDB was 0.16 nmol/g. (b) The threshold value of Ki-67 by QDB was 11.9 nmol/g. (c) NIPI-QDB had statistical significance. (d) The AUC of NIPI-QDB was 0.8. NIPI: Novel international prognostic index, QDB: Quantitative dot blot, AUC: Area under curve.

Article Snippet: 2 , Ki-67 (clone number: MIB-1; 20220920) , Beijing Zhongshan Jinqiao Biotechnology Co., Ltd..

Techniques: Diagnostic Assay, Quantitative Dot Blot

Journal: bioRxiv

Article Title: Chemotherapy-Induced Oral Mucosal Injury Is Defined by p53 Activation, Cell Cycle Arrest and Diverse Epithelial Progenitor Dynamics

doi: 10.64898/2026.04.06.716752

Figure Lengend Snippet: (A) Heatmaps showing differentially expressed p53 targets in tongue and intestine in 5-FU-treated mice compared to PBS as determined via bulk RNA sequencing. Only genes with log2 fold-change (log2FC) > 1 or < –1 and adjusted p values < 0.05 are shown. (B-C) Representative micrographs and quantification of KI67 staining indicating proliferative cells in tongue mucosa (B) and small intestine (C) of PBS– and 5-FU-treated mice. (D) Western blot of total and cleaved Caspase-3 protein in tongue tissue lysates of PBS– and 5-FU-treated mice. Positive control (PC) is Jurkat cells treated with 1 μM staurosporine, and Negative control (NC) is untreated Jurkat cells. (E) Representative micrographs of sections corresponding to tongue lesion areas in mice treated with 5-FU and sacrificed at day 4 showing H&E staining and immunohistochemistry for detection of KI67 and cleaved Caspase 3 protein (Scale bar = 50 µm). Data in B and C are presented as mean±SD; *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, as determined via ANOVA with Tukey post hoc analysis.

Article Snippet: Slides were incubated with an anti-cleaved Caspase-3 (Asp175) antibody (Cell Signaling Technology, Danvers, MA, USA; Cat# 9661, RRID:AB_2341188) at 1:150 or an anti-KI67 antibody (R&D Systems, Minneapolis, MN, USA; Cat# MAB7617) at 1:125 for 20 min followed by Rabbit Envision (Dako, Carpinteria, CA, USA; Cat# K4003) for 30 min. Diaminobenzidine (DAB) was applied for 10 minutes for visualization.

Techniques: RNA Sequencing, Staining, Western Blot, Positive Control, Negative Control, Immunohistochemistry