Journal: Materials & Design

Article Title: Hyaluronic acid methacrylate microneedles loaded with astaxanthin alleviate rheumatoid arthritis by downregulating the PI3K-AKT pathway

doi: 10.1016/j.matdes.2025.114571

Figure Lengend Snippet: Fig. 2. ATX inhibits proliferation, migration and invasion capabilities of MH7A cells. A: colony assay in different groups. B: migration assay in different groups. C: transwell assay in different groups. D: WB analysis of P-PI3K and P-AKT in different groups. *p < 0.05,**p < 0.01,***p < 0.001.

Article Snippet: For evaluating the biocompatibility of HAMA/ATX MNs, the cytoskeleton of MH7A cells was stained with a commercial kit (Yeasen Biotechnology Co., Ltd., Shanghai, China).

Techniques: Migration, Colony Assay, Transwell Assay

Journal: Materials & Design

Article Title: Hyaluronic acid methacrylate microneedles loaded with astaxanthin alleviate rheumatoid arthritis by downregulating the PI3K-AKT pathway

doi: 10.1016/j.matdes.2025.114571

Figure Lengend Snippet: Fig. 3. ATX inhibits MH7A cells proliferation, migration and invasion thriough PI3K-AKT pathway. A: colony assay in different groups. B: migration assay in different groups. C: transwell assay in different groups. D: WB analysis of P-PI3K and P-AKT in different groups. *p < 0.05,**p < 0.01,***p < 0.001.

Article Snippet: For evaluating the biocompatibility of HAMA/ATX MNs, the cytoskeleton of MH7A cells was stained with a commercial kit (Yeasen Biotechnology Co., Ltd., Shanghai, China).

Techniques: Migration, Colony Assay, Transwell Assay

Journal: Journal of Nanobiotechnology

Article Title: An orally-administered nanotherapeutics with gold nanospheres supplying for rheumatoid arthritis therapy by re-shaping gut microbial tryptophan metabolism

doi: 10.1186/s12951-025-03450-7

Figure Lengend Snippet: IPA/IAA mixture inhibited proliferation, migration/invasion and promotes apoptosis of MH7A cells. A The proliferation of MH7A cells was assessed using the CCK8 assay. IPA/IAA mixture treatment significantly inhibited the proliferation of MH7A cells in a concentration-dependent manner (0, 1, 5, 10 μM). B Flow cytometry analysis of apoptosis. MH7A cells were treated with IPA/IAA mixture (10 µM) and analyzed for apoptosis by flow cytometry. C Quantification of apoptosis. D Representative images of EdU staining for the proliferation of MH7A cells. E Quantification of EdU-positive cells. F Representative images of transwell assay for the migration and invasion of MH7A cells. G and H Relative ratio of cell migration and invasion

Article Snippet: To measure the proliferation of MH7A cells, a Cell Counting Kit-8 (Cat#: C0038, Beyotime) was utilized according to the manufacturer’s instructions.

Techniques: Migration, CCK-8 Assay, Concentration Assay, Flow Cytometry, Staining, Transwell Assay

Journal: Journal of Nanobiotechnology

Article Title: An orally-administered nanotherapeutics with gold nanospheres supplying for rheumatoid arthritis therapy by re-shaping gut microbial tryptophan metabolism

doi: 10.1186/s12951-025-03450-7

Figure Lengend Snippet: IPA/IAA mixture significantly inhibited NF-κB signaling pathway via the upregulation of PTEN. A PCA analysis showed distinct clustering between IPA/IAA-treated and control groups. B Heatmap of Differentially expressed genes (DEGs) in the IPA + IAA group compared to the control group. Different colors represent different levels of gene relative expression, ranging from blue through white to red, indicating expression levels that range from low to high. Red indicates highly expressed genes, and blue shows lowly expressed genes. C Bar plot of DEGs between IPA + IAA and CT groups (|fold change|> 1.35, p < 0.05). D and E Positive regulation of cell migration (enrichment score = − 0.48) and focal adhesion (enrichment score = − 0.47) pathways were down-regulated in the IPA/IAA group compared to the CT group as validated by GSEA. F KEGG enrichment bar plot. The y-axis represents the names of the top 20 KEGG pathways with the smallest p-values, and the x-axis represents the -log10 value of the p-value from the KEGG pathway enrichment analysis. G PI3K/AKT pathway (enrichment score = − 0.38) was down-regulated in IPA/IAA as validated by GSEA. H Volcano plot of DEGs in the IPA + IAA group compared to the CT group. PTEN was significantly upregulated in the IPA + IAA group. I The relative expression of PTEN between CT and IPA + IAA groups. J Relative expression of p-p65, p65, p-IκBα, IκBα, p-PTEN, and PTEN in the presence or absence of IPA/IAA mixture during the stimulation for 0–120 min at the protein level. β-actin was used as an internal control. K–P Quantitative analyses of p-p65, p65, p-IκBα, IκBα, p-PTEN, and PTEN expression. Q Representative images of the intracellular location of the NF-κB p65. R MH7A cells were cultured with or without the IPA/IAA mixture for 12 h and treated with cycloheximide (CHX) (50 μg/mL). Total cell lysates were subjected to immunoblotting analysis. S Quantitative analyses of PTEN expression treated with CHX in the presence or absence of IPA/IAA mixture (0, 4, 8, 12 h). T MH7A cells were pretreated with IPA/IAA mixture for 8 h, with or without MG132 (50 μM) for 2 h. The cell lysates were immunoprecipitated with PTEN antibody and immunoblotted with ubiquitin and PTEN antibodies. U–V Relative expression levels of ubiquitin in PTEN

Article Snippet: To measure the proliferation of MH7A cells, a Cell Counting Kit-8 (Cat#: C0038, Beyotime) was utilized according to the manufacturer’s instructions.

Techniques: Control, Expressing, Migration, Cell Culture, Western Blot, Immunoprecipitation, Ubiquitin Proteomics