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results of the methylation analysis of the MGMT gene promoter in eight glioblastomas

Journal: The Pan African Medical Journal

Article Title: Detection of O6-methylguanine-DNA methyltransferase gene methylation status in IDH-wild type glioblastomas using methylation-specific qPCR: a first report from Morocco

doi: 10.11604/pamj.2024.49.110.45250

Figure Lengend Snippet: results of the methylation analysis of the MGMT gene promoter in eight glioblastomas

Article Snippet: The PCR Kit used was Genmark ́s MGMT Methylation Analysis Kit and contained the following reagents; 2X Master Mix (Taq DNA polymerase, dNTPs, MgCl2, and reaction buffer), 4X MGMT Primer Probe Mix, positive control (PC) 100% methylated DNA, and MGMT Calibrator 2% methylated DNA (theoretical).

Techniques: Methylation