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NEK8 promotes GC cell clonal formation, wound healing, invasion, and migration in vitro. A NEK8 overexpression enhanced the colony-forming capability of GC cells. B NEK8 knockdown significantly inhibited the colony-forming capability of GC cells. C , D Scratch healing assays showed that NEK8 overexpression markedly increased the horizontal migration capabilities of HGC27 cells. E – H Scratch healing assays further demonstrated that NEK8 knockdown significantly reduced the horizontal migration capabilities of SGC7901 and MGC803 cells. I , J NEK8 overexpression enhanced the invasive and migratory capabilities of HGC27 cells. K – N Transwell assays indicated that NEK8 knockdown significantly decreased the invasive and migratory capabilities of SGC7901 and MGC803 cells. *P < 0.05, **P < 0.01, ***P < 0.001

Journal: Molecular Medicine

Article Title: NEK8 promotes the progression of gastric cancer by reprogramming asparagine metabolism

doi: 10.1186/s10020-024-01062-9

Figure Lengend Snippet: NEK8 promotes GC cell clonal formation, wound healing, invasion, and migration in vitro. A NEK8 overexpression enhanced the colony-forming capability of GC cells. B NEK8 knockdown significantly inhibited the colony-forming capability of GC cells. C , D Scratch healing assays showed that NEK8 overexpression markedly increased the horizontal migration capabilities of HGC27 cells. E – H Scratch healing assays further demonstrated that NEK8 knockdown significantly reduced the horizontal migration capabilities of SGC7901 and MGC803 cells. I , J NEK8 overexpression enhanced the invasive and migratory capabilities of HGC27 cells. K – N Transwell assays indicated that NEK8 knockdown significantly decreased the invasive and migratory capabilities of SGC7901 and MGC803 cells. *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: GES-1 normal gastric epithelial cells, along with the GC cell lines AGS, SGC7901, MGC803, and HGC27, were sourced from Genechem (Shanghai, China).

Techniques: Migration, In Vitro, Over Expression, Knockdown