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Cell viability (%) of MG63 and Saos-2 cells treated with pexidartinib (via PLX3397) and sotuletinib.

Journal: Oncology Letters

Article Title: Computational discovery of novel colony-stimulating factor-1 receptor as a potential therapeutic biomarker in osteosarcoma and a novel inhibitor from herbal sources

doi: 10.3892/ol.2026.15618

Figure Lengend Snippet: Cell viability (%) of MG63 and Saos-2 cells treated with pexidartinib (via PLX3397) and sotuletinib.

Article Snippet: MG63 osteosarcoma cells were purchased from Procell Life Science Technology Co., Ltd. (cat. no. #CL-0157; http://www.procell.com.cn/p/mg-63-cl-0157-72575 ).

Techniques:

Sensitization of the MG63 and Saos-2 cell lines to a CSF1R inhibitor. (A) Dose-dependent cytotoxic effects of linarin on MG63 and Saos-2 osteosarcoma cell viability. (B) Dose-dependent cytotoxic effects of saikosaponin C on MG63 and Saos-2 osteosarcoma cell viability. (C) Dose-dependent cytotoxic effects of sarsasapogenin on MG63 and Saos-2 osteosarcoma cell viability. (D) Molecular docking of the binding between sarsasapogenin and the CSF1R protein. (E) Visual molecular docking of the binding acid residues between sarsasapogenin and CSF1R protein. (F) The expression levels of CSF1R as detected by western blot analysis in MG63 and Saos-2 cells after treatment with sarsasapogenin. *P<0.05. CSF1R, colony-stimulating factor-1 receptor.

Journal: Oncology Letters

Article Title: Computational discovery of novel colony-stimulating factor-1 receptor as a potential therapeutic biomarker in osteosarcoma and a novel inhibitor from herbal sources

doi: 10.3892/ol.2026.15618

Figure Lengend Snippet: Sensitization of the MG63 and Saos-2 cell lines to a CSF1R inhibitor. (A) Dose-dependent cytotoxic effects of linarin on MG63 and Saos-2 osteosarcoma cell viability. (B) Dose-dependent cytotoxic effects of saikosaponin C on MG63 and Saos-2 osteosarcoma cell viability. (C) Dose-dependent cytotoxic effects of sarsasapogenin on MG63 and Saos-2 osteosarcoma cell viability. (D) Molecular docking of the binding between sarsasapogenin and the CSF1R protein. (E) Visual molecular docking of the binding acid residues between sarsasapogenin and CSF1R protein. (F) The expression levels of CSF1R as detected by western blot analysis in MG63 and Saos-2 cells after treatment with sarsasapogenin. *P<0.05. CSF1R, colony-stimulating factor-1 receptor.

Article Snippet: MG63 osteosarcoma cells were purchased from Procell Life Science Technology Co., Ltd. (cat. no. #CL-0157; http://www.procell.com.cn/p/mg-63-cl-0157-72575 ).

Techniques: Binding Assay, Expressing, Western Blot