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pyrosequencing methylation analyses  (Pyrosequencing Inc)
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Pyrosequencing Inc pyrosequencing methylation analyses
Propagation of human mammary epithelial cells. a Growth kinetics of 12-breast primary epithelial cells ( 12BPEC ) and 14BPEC (population doubling ( PD ) vs days in culture). b Frequencies of 12BPEC at PD8 and PD41 expressing senescence-associated β-galactosidase marker. c <t>Methylation</t> mean of the p16 INK4a gene promoter in 12BPECs at different population doublings, as obtained by pyrosequencing methylation analyses. The red horizontal line represents the frequency of p16 INK4a promoter methylation in post-M0 human mammary epithelial cells. d Expression profile of p16 and p53 in 12BPEC at pre-stasis, stasis and post-stasis population doublings. Expression was tested using qRT-PCR, and data were normalized using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) RNA levels
Pyrosequencing Methylation Analyses, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/methylation+pyrosequencing+analyses/pyrosequencing+based+methylation+analysis/pmc04711177-204-18-17
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pyrosequencing methylation analyses - by Bioz Stars, 2026-11
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methylation pyrosequencing analyses  (Zymo Research)
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Zymo Research methylation pyrosequencing analyses
Propagation of human mammary epithelial cells. a Growth kinetics of 12-breast primary epithelial cells ( 12BPEC ) and 14BPEC (population doubling ( PD ) vs days in culture). b Frequencies of 12BPEC at PD8 and PD41 expressing senescence-associated β-galactosidase marker. c <t>Methylation</t> mean of the p16 INK4a gene promoter in 12BPECs at different population doublings, as obtained by pyrosequencing methylation analyses. The red horizontal line represents the frequency of p16 INK4a promoter methylation in post-M0 human mammary epithelial cells. d Expression profile of p16 and p53 in 12BPEC at pre-stasis, stasis and post-stasis population doublings. Expression was tested using qRT-PCR, and data were normalized using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) RNA levels
Methylation Pyrosequencing Analyses, supplied by Zymo Research, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/methylation+pyrosequencing+analyses/pmc04063827-80-0-4
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methylation pyrosequencing analyses - by Bioz Stars, 2026-11
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Propagation of human mammary epithelial cells. a Growth kinetics of 12-breast primary epithelial cells ( 12BPEC ) and 14BPEC (population doubling ( PD ) vs days in culture). b Frequencies of 12BPEC at PD8 and PD41 expressing senescence-associated β-galactosidase marker. c Methylation mean of the p16 INK4a gene promoter in 12BPECs at different population doublings, as obtained by pyrosequencing methylation analyses. The red horizontal line represents the frequency of p16 INK4a promoter methylation in post-M0 human mammary epithelial cells. d Expression profile of p16 and p53 in 12BPEC at pre-stasis, stasis and post-stasis population doublings. Expression was tested using qRT-PCR, and data were normalized using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) RNA levels

Journal: Breast Cancer Research : BCR

Article Title: Breast primary epithelial cells that escape p16-dependent stasis enter a telomere-driven crisis state

doi: 10.1186/s13058-015-0667-z

Figure Lengend Snippet: Propagation of human mammary epithelial cells. a Growth kinetics of 12-breast primary epithelial cells ( 12BPEC ) and 14BPEC (population doubling ( PD ) vs days in culture). b Frequencies of 12BPEC at PD8 and PD41 expressing senescence-associated β-galactosidase marker. c Methylation mean of the p16 INK4a gene promoter in 12BPECs at different population doublings, as obtained by pyrosequencing methylation analyses. The red horizontal line represents the frequency of p16 INK4a promoter methylation in post-M0 human mammary epithelial cells. d Expression profile of p16 and p53 in 12BPEC at pre-stasis, stasis and post-stasis population doublings. Expression was tested using qRT-PCR, and data were normalized using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) RNA levels

Article Snippet: Methylation mean of the p16 INK4a promoter gene in 14BPECs at different population doublings, as obtained by pyrosequencing methylation analyses.

Techniques: Expressing, Marker, Methylation, Quantitative RT-PCR