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Immunofluorescence shows co-localization of ADAMTS-1 and nucleophosmin in MCF-10A, MCF-7 and MDA-MB-231 cell lines (A). MDA-MB-231 image used for colocalization analysis (B), with the white line used to generate the <t>linescan</t> plot (C). Linescan profiles display a clear overlap between ADAMTS-1 (Red) and nucleophosmin (Green). The colocalization color map is illustrated in C, where hot colors represent positive correlation (colocalization), and cold colors represent exclusion. Scatter plot (D) of ADAMTS-1 (red, x-axis) and nucleophosmin (green, y-axis) with the linear regression line. Points of scatterplot cluster around regression line. ADAMTS-1 (red), nucleophosmin (green) and nuclei (blue). Scale bar, 5 μm.
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Immunofluorescence shows co-localization of ADAMTS-1 and nucleophosmin in MCF-10A, MCF-7 and MDA-MB-231 cell lines (A). MDA-MB-231 image used for colocalization analysis (B), with the white line used to generate the linescan plot (C). Linescan profiles display a clear overlap between ADAMTS-1 (Red) and nucleophosmin (Green). The colocalization color map is illustrated in C, where hot colors represent positive correlation (colocalization), and cold colors represent exclusion. Scatter plot (D) of ADAMTS-1 (red, x-axis) and nucleophosmin (green, y-axis) with the linear regression line. Points of scatterplot cluster around regression line. ADAMTS-1 (red), nucleophosmin (green) and nuclei (blue). Scale bar, 5 μm.

Journal: PLoS ONE

Article Title: ADAMTS-1 Is Found in the Nuclei of Normal and Tumoral Breast Cells

doi: 10.1371/journal.pone.0165061

Figure Lengend Snippet: Immunofluorescence shows co-localization of ADAMTS-1 and nucleophosmin in MCF-10A, MCF-7 and MDA-MB-231 cell lines (A). MDA-MB-231 image used for colocalization analysis (B), with the white line used to generate the linescan plot (C). Linescan profiles display a clear overlap between ADAMTS-1 (Red) and nucleophosmin (Green). The colocalization color map is illustrated in C, where hot colors represent positive correlation (colocalization), and cold colors represent exclusion. Scatter plot (D) of ADAMTS-1 (red, x-axis) and nucleophosmin (green, y-axis) with the linear regression line. Points of scatterplot cluster around regression line. ADAMTS-1 (red), nucleophosmin (green) and nuclei (blue). Scale bar, 5 μm.

Article Snippet: Colocalization analysis was carried out by the Linescan tool (Metamorph Premier 7.6 software), and the Image J plugins JaCop, Colocalization colormap and Colocalization threshold.

Techniques: Immunofluorescence