Journal: Scientific Reports

Article Title: Supraphysiological Levels of Oxygen Exposure During the Neonatal Period Impairs Signaling Pathways Required for Learning and Memory

doi: 10.1038/s41598-018-28220-4

Figure Lengend Snippet: Histological assessment of effect hyperoxia on cell proliferation, reactive astrocytes and myelin protein. Photomicrographs of PCNA stained brain sections from P14 mice exposed to Air ( a ) or Hyperoxia ( b ). ( c ) Graphical representation of PCNA staining results; hyperoxia-exposed mice had a reduction in the number of proliferative cells in the dentate gyrus. Photomicrographs of GFAP stained brain sections from P14 mice exposed to Air ( d ) or Hyperoxia ( e ). ( f ) Graphical of representation GFAP staining results; hyperoxia-exposed mice had an increased in the percentage of GFAP stained area in the CA1 region. Photomicrographs of CNPase stained brain sections from P14 mice exposed to Air ( g ) or Hyperoxia ( h ). ( i ) Graphical representation of CNAPase staining results; hyperoxia exposed mice had decreased in the percentage of CNPase stained area in the CA1 region of the hippocampus. In the panels (c,f), and (i); Air: solid green bars, Hyperoxia: solid red bars with horizontal stripes. *Represents p < 0.05; Air vs. Hyperoxia.

Article Snippet: Using MetaMorph v.6.2 software, the number of cells stained for PCNA were counted (non-stereological method), while the area stained for GFAP and CNPase were analyzed using intensity thresholding and expressed as a percentage of total area as described previously .

Techniques: Staining