Journal: International Journal of Pharmaceutics: X

Article Title: Allicin-based biomimetic nanoparticles of the erythrocyte membrane for the delivery of lumefantrine to enhance its antimalarial effect

doi: 10.1016/j.ijpx.2026.100487

Figure Lengend Snippet: Neutralization of merozoites. A Purification of infected erythrocytes using the Percoll separation solution, displaying infected (black arrow) and normal (red arrow) erythrocytes. B Confocal fluorescent images of merozoites (blue), PECm-Allicin@LM (red), and their colocalization (purple). Scale bar = 50 μm. C Representative scatter plots of Hoechst 33342/CFDA-SE for the invasion test of merozoites and normal erythrocytes after drug treatment. D Intrusion rate (the percentage of cells in the Q2 area in each group relative to that in the Q2 area in the Model group). Data are presented as mean ± SEM ( n = 3), * P < 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: The red fluorescent cell membrane dye DiD was purchased from Shanghai Titan Scientific Co., Ltd. (China).

Techniques: Neutralization, Purification, Infection

Journal: bioRxiv

Article Title: Estrogen-related receptor gamma is required for normal auditory innervation and is essential for hearing

doi: 10.64898/2026.05.02.722410

Figure Lengend Snippet: Maximum intensity projections of confocal z-stacks of cochlear whole mount preparations from Esrrg +/+ (A,C,E and G) and Esrrg fl/fl ;Sox10- Cre (B,D,F and H) mice immunolabelled with antibodies to Myosin7a (Blue) and NF-H (Green), co-stained with the cell plasma membrane dye CellMask™ orange (Magenta) to reveal the myelinated fibres. Esrrg fl/fl ;Sox10- Cre mice display defective myelination from early development (H) with reduced auditory fibres apparent at P1 (F) . (I) Abundance of myelinated fibres extracted from the fluorescent intensity of the CellMask™ orange signal at 8kHz and 24kHz from a 50µm by 59µm ROI in the OSL distal to the HP. ROI were acquired from 20x maximum intensity projections as shown in A, B. Data was acquired from 2-3 mice per genotype per sex. Maximum intensity projections of confocal z-stacks of apical coil cochlear cryosections from adult Esrrg +/+ (J) and Esrrg fl/fl ;Sox10- Cre (K) mice immunolabelled with antibodies to the heminodal proteins - Caspr (Magenta) and AnkG (Green). Maximum intensity projections of confocal z-stacks of cochlear whole mount preparations from adult Esrrg +/+ (L) and Esrrg fl/fl ;Sox10- Cre (M) mice immunolabelled with antibodies to the efferent innervation marker ChAT (green) and Myo7a (Magenta). Maximum intensity projections of confocal z-stacks of mid-coil cochlear cryosections from adult Esrrg +/+ (N) and Esrrg fl/fl ;Sox10- Cre (O) mice immunolabelled with antibodies to the pan-neuronal SGN marker βIII-Tubulin (Green) and the Type II marker Peripherin (Magenta). Note, Rosenthal’s canal (dashed lines) is smaller in Esrrg fl/fl ;Sox10- Cre mice (O) . (P-R) SGN density at P29 in Esrrg fl/fl ;Sox10- Cre mice compared to controls across basal, mid, and apical cochlear coils: (P) Type I & II, (Q) Type I and (R) Type II SGNs. Data was pooled from both male and female mice and acquired from 3 mice per genotype per sex. Data is plotted as mean values ± SD; *p<0.05; **p<0.01; ***p<0.001 unpaired t-test. Images represent data from 2-3 mice per genotype per sex. Scale bar: A,B: 20μm; C,D:100 μm; E,F: 20μm; G,H: 10μm; J,K: 10 μm; L,M: 10μm; N,O: 20 μm.

Article Snippet: In some experiments, 10nM Phalloidin-Atto 647N (Sigma-Aldrich, Gillingham, U.K.) was added to the secondary antibody solution to label f- actin for visualisation of hair cell stereocilia or CellMaskTM Orange (Invitrogen, #C10045) a fluorescent plasma membrane dye, diluted 1:5000 in 0.3% Triton-X 100 with PBS, was added as an extra 5-minute room temperature incubation after the secondary antibody step to visualise the myelin sheaths.

Techniques: Staining, Clinical Proteomics, Membrane, Marker

Journal: Infection and Immunity

Article Title: Loss of Fgr41 in Candida albicans attenuates virulence and increases proinflammatory immune responses in a manner that is dependent on β(1,3)-glucan but not dectin-1

doi: 10.1128/iai.00523-25

Figure Lengend Snippet: Cell separation defects lead to phagocytosis of larger clumps. RAW264.7 macrophages were co-incubated with UV-inactivated C. albicans for 2 h and fixed with 4% formaldehyde. Macrophages were stained with CellBrite Green, and C. albicans cells were stained with CFW. ( A ) Confocal microscopy images of macrophages co-incubated with wild type, eng1Δ/Δ, fgr41Δ/Δ, fgr41Δ/ΔENG1 oe , or eng1Δ/Δfgr41Δ/Δ strains. Scale bar = 25 µm. ( B–D ) Quantification of panel A. A total of 40 images were analyzed for each strain. C. albicans cells were counted as being phagocytosed if they appeared internal to the macrophage and/or caused a gap in green fluorescence in the macrophage. ( B ) Percentages of macrophages containing each number of C. albicans cells in panel A. n = the total number of macrophages with at least one C. albicans cell inside them. Graphs including macrophages that had not phagocytosed any C. albicans are found in . ( C ) The number of C. albicans cells inside each macrophage for each strain from panel A. Each point represents a macrophage that had phagocytosed at least one C. albicans cell. The number of macrophages represented in each bar corresponds to the n in panel B. **** P <0.0001, *** P < 0.001, by one-way ANOVA, ns = not significant. ( D ) The wild type and eng1Δ/Δ bars from panel C were analyzed in isolation, which revealed a significant difference (* P < 0.05, by Welch’s t -test).

Article Snippet: CellBrite Green stain was purchased from Biotium (30021).

Techniques: Incubation, Staining, Confocal Microscopy, Fluorescence, Isolation