Journal: Progress in Biomedical Engineering

Article Title: The potential of in vitro neuronal networks cultured on micro electrode arrays for biomedical research

doi: 10.1088/2516-1091/acce12

Figure Lengend Snippet: Figure 1. (a), (b) Overview of medium- and long-term in vitro neuronal models, divided into (a) rodent neuronal cultures, including 2D and 3D cultures of dissociated neuronal cells, and organotypic brain slices, and (b) hiPSCs-derived neuronal cultures, including 2D cultures, brain organoids, and 3D cultures. (c), (d) Two main electrophysiological techniques used to record neuronal activity of in vitro neuronal cultures, i.e. (c) patch-clamp, recording the electrophysiological activity of single neurons at single-cell level, and (d) MEAs, recording the electrophysiological activity of neuronal cultures at network level. (e) Different types of commercially available MEA devices for recording electrophysiological activity of in vitro neuronal cultures, i.e. single-well MEAs (swMEAs), multi-well MEAs (mwMEAs), CMOS-based high-density MEAs (HD-MEAs), MEAs with non-planar micro-electrodes, and three-dimensional MEAs (3D MEAs). Created with BioRender.com.

Article Snippet: Beside conventional patch-clamp, allowing the measurement of neuronal activity at single-cell level [12–14], micro electrode arrays (MEAs) (i.e. cell culture dishes with embedded micro-electrodes [15–17]) have been increasingly used to characterize neuronal activity at the network level.

Techniques: In Vitro, Derivative Assay, Activity Assay, Patch Clamp

Journal: Progress in Biomedical Engineering

Article Title: The potential of in vitro neuronal networks cultured on micro electrode arrays for biomedical research

doi: 10.1088/2516-1091/acce12

Figure Lengend Snippet: Figure 3. Schematic raster plots describing the effect of low and high concentrations of glutamate and GABA on the spontaneous activity of neuronal networks on MEAs. NBs are highlighted in light blue. Created with BioRender.com.

Article Snippet: Beside conventional patch-clamp, allowing the measurement of neuronal activity at single-cell level [12–14], micro electrode arrays (MEAs) (i.e. cell culture dishes with embedded micro-electrodes [15–17]) have been increasingly used to characterize neuronal activity at the network level.

Techniques: Activity Assay

Journal: Progress in Biomedical Engineering

Article Title: The potential of in vitro neuronal networks cultured on micro electrode arrays for biomedical research

doi: 10.1088/2516-1091/acce12

Figure Lengend Snippet: Figure 6. Schematic raster plots describing the phenotype on MEAs showed by affected neuronal networks in comparison with healthy neuronal networks, suggesting hypothesis about the molecular and cellular mechanisms underlying pathophysiology. NBs are highlighted in light blue. Created with BioRender.com.

Article Snippet: Beside conventional patch-clamp, allowing the measurement of neuronal activity at single-cell level [12–14], micro electrode arrays (MEAs) (i.e. cell culture dishes with embedded micro-electrodes [15–17]) have been increasingly used to characterize neuronal activity at the network level.

Techniques: Comparison