Journal: iScience

Article Title: Microenvironmental acidosis drives PARP- and ATM inhibitor resistance in p53 deficient pancreatic cancer

doi: 10.1016/j.isci.2026.115112

Figure Lengend Snippet: Acid adaptation and p53 KO alter protein expression of acid-base transporters At week 11, WT and p53KO organoids grown under Ctrl, AA, or AA->7.4 conditions as in were lysed and subjected to western blot analysis for proteins relevant to acid-base homeostasis. Representative western blots are shown on the left, panels on the right show the corresponding densitometric quantifications, as means with S.E.M. error bars and biological replicates shown as open circles. β-actin is used as loading control. A(i) NHE1, NBCn1 western blots, (ii) and (iii), Quantifications of relative NHE1 and NBCn1 levels, respectively. B(i) MCT1, MCT4 western blots, (ii) and (iii), Quantifications of relative MCT1 and MCT4 levels, respectively. C(i) Slc26a6, CFTR western blots, (ii) and (iii), Quantifications of relative Slc26a6 and CFTR levels, respectively. Data represent 3–8 independent biological repeats per condition/protein. Statistical analysis: two-way ANOVA, with Tukey’s post test.

Article Snippet: MCT1 , Santa Cruz Biotechnology , Sc-365501, RRID: AB_2943297.

Techniques: Expressing, Western Blot, Control