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pu6 sgrna ef1α puro t2a mcherry vector  (Addgene inc)


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    Structured Review

    Addgene inc pu6 sgrna ef1α puro t2a mcherry vector
    Pu6 Sgrna Ef1α Puro T2a Mcherry Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcherry+control+vector/pU6-sgRNA+EF1Alpha-puro-T2A-BFP%2EControl+(Plasmid+%23128749)/bio_rxiv__64898__2025__12__29__696402-248-23-26
    Average 93 stars, based on 2 article reviews
    pu6 sgrna ef1α puro t2a mcherry vector - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Over Expression:

    Article Title: Early and Late Chaperone Intervention Therapy Boosts XBP1s and ADAM10, Restores Proteostasis, and Rescues Learning in Alzheimer’s Disease Mice
    Article Snippet: .. Stereotaxic hippocampal injectionswere performed to deliver a BiP viral overexpression vector (Vector Biolabs; AAV5-CamKIIaGRP78; titer 4.8 × 1012 GC/ml) and an mCherry control vector (Addgene; pAAV5-CaMKIIa-mCherry; titer 2.3 × 10−13 GC/ml) into the hippocampi of young and aged mice, as previously described30. ..

    Article Title: Early and late chaperone intervention therapy boosts XBP1s and ADAM10, restores proteostasis, and rescues learning in Alzheimer’s Disease mice
    Article Snippet: .. Stereotaxic hippocampal injections were performed to deliver a BiP viral overexpression vector (Vector Biolabs; AAV5-CamKIIa-GRP78; titer 4.8×10 12 GC/ml) and an mCherry control vector (Addgene; pAAV5-CaMKIIa-mCherry; titer 2.3 x10 -13 GC/ml) into the hippocampi of young and aged mice as previously described ( ). ..

    Article Title: Reducing ER stress with chaperone therapy reverses sleep fragmentation and cognitive decline in aged mice
    Article Snippet: Data were scored with SleepSign Analysis Software (Kissei Comtec Co., LTD), and spectral data were analyzed as previously described (Franken et al., ; Hasan et al., ; Lim et al., ; Naidoo et al., ; Perron et al., ), with additional code generated by Dr. Isaac Perron (python, https://pypi.org/project/eeg‐sleep‐analysis/ ). .. Stereotaxic hippocampal injections were performed to deliver a BiP viral overexpression vector (Vector Biolabs; AAV5‐CamKIIa‐GRP78; titer 4.8 × 10 12 GC/ml) and an mCherry control vector (Addgene; pAAV5‐CaMKIIa‐mCherry; titer 2.3 × 10 −13 GC/ml) into the hippocampi of young and aged mice. ..

    Plasmid Preparation:

    Article Title: Early and Late Chaperone Intervention Therapy Boosts XBP1s and ADAM10, Restores Proteostasis, and Rescues Learning in Alzheimer’s Disease Mice
    Article Snippet: .. Stereotaxic hippocampal injectionswere performed to deliver a BiP viral overexpression vector (Vector Biolabs; AAV5-CamKIIaGRP78; titer 4.8 × 1012 GC/ml) and an mCherry control vector (Addgene; pAAV5-CaMKIIa-mCherry; titer 2.3 × 10−13 GC/ml) into the hippocampi of young and aged mice, as previously described30. ..

    Article Title: Nuclear mechanosensing drives chromatin remodelling in persistently activated fibroblasts.
    Article Snippet: 1Materials Science and Engineering Program, University of Colorado Boulder, Boulder, CO, USA.. 2BioFrontiers Institute, University of Colorado Boulder, Boulder, CO, USA.. 3Department of Molecular, Cellular and Developmental Biology, University of Colorado Boulder, Boulder, CO, USA.

    Article Title: Early and late chaperone intervention therapy boosts XBP1s and ADAM10, restores proteostasis, and rescues learning in Alzheimer’s Disease mice
    Article Snippet: .. Stereotaxic hippocampal injections were performed to deliver a BiP viral overexpression vector (Vector Biolabs; AAV5-CamKIIa-GRP78; titer 4.8×10 12 GC/ml) and an mCherry control vector (Addgene; pAAV5-CaMKIIa-mCherry; titer 2.3 x10 -13 GC/ml) into the hippocampi of young and aged mice as previously described ( ). ..

    Article Title: Nuclear mechanosensing drives chromatin remodelling in persistently activated fibroblasts
    Article Snippet: .. The mCherry control vector and DNKASH were a gift from J. Lammerding, pcDNA Nesprin-HL (Addgene plasmid no. 68128; http://n2t.net/addgene:68128 ; RRID:Addgene_68128) and pcDNA Nesprin-TS (Addgene plasmid no. 68127; http://n2t.net/addgene:68127 ; RRID:Addgene_68127) were a gift from D. Conway, and mTFP1-Lifeact-7 was a gift from R. Campbell and M. Davidson (Addgene plasmid no. 54749; http://n2t.net/addgene:54749 ; RRID:Addgene_54749). ..

    Article Title: Reducing ER stress with chaperone therapy reverses sleep fragmentation and cognitive decline in aged mice
    Article Snippet: Data were scored with SleepSign Analysis Software (Kissei Comtec Co., LTD), and spectral data were analyzed as previously described (Franken et al., ; Hasan et al., ; Lim et al., ; Naidoo et al., ; Perron et al., ), with additional code generated by Dr. Isaac Perron (python, https://pypi.org/project/eeg‐sleep‐analysis/ ). .. Stereotaxic hippocampal injections were performed to deliver a BiP viral overexpression vector (Vector Biolabs; AAV5‐CamKIIa‐GRP78; titer 4.8 × 10 12 GC/ml) and an mCherry control vector (Addgene; pAAV5‐CaMKIIa‐mCherry; titer 2.3 × 10 −13 GC/ml) into the hippocampi of young and aged mice. ..

    Control:

    Article Title: Early and Late Chaperone Intervention Therapy Boosts XBP1s and ADAM10, Restores Proteostasis, and Rescues Learning in Alzheimer’s Disease Mice
    Article Snippet: .. Stereotaxic hippocampal injectionswere performed to deliver a BiP viral overexpression vector (Vector Biolabs; AAV5-CamKIIaGRP78; titer 4.8 × 1012 GC/ml) and an mCherry control vector (Addgene; pAAV5-CaMKIIa-mCherry; titer 2.3 × 10−13 GC/ml) into the hippocampi of young and aged mice, as previously described30. ..

    Article Title: Nuclear mechanosensing drives chromatin remodelling in persistently activated fibroblasts.
    Article Snippet: 1Materials Science and Engineering Program, University of Colorado Boulder, Boulder, CO, USA.. 2BioFrontiers Institute, University of Colorado Boulder, Boulder, CO, USA.. 3Department of Molecular, Cellular and Developmental Biology, University of Colorado Boulder, Boulder, CO, USA.

    Article Title: Early and late chaperone intervention therapy boosts XBP1s and ADAM10, restores proteostasis, and rescues learning in Alzheimer’s Disease mice
    Article Snippet: .. Stereotaxic hippocampal injections were performed to deliver a BiP viral overexpression vector (Vector Biolabs; AAV5-CamKIIa-GRP78; titer 4.8×10 12 GC/ml) and an mCherry control vector (Addgene; pAAV5-CaMKIIa-mCherry; titer 2.3 x10 -13 GC/ml) into the hippocampi of young and aged mice as previously described ( ). ..

    Article Title: Nuclear mechanosensing drives chromatin remodelling in persistently activated fibroblasts
    Article Snippet: .. The mCherry control vector and DNKASH were a gift from J. Lammerding, pcDNA Nesprin-HL (Addgene plasmid no. 68128; http://n2t.net/addgene:68128 ; RRID:Addgene_68128) and pcDNA Nesprin-TS (Addgene plasmid no. 68127; http://n2t.net/addgene:68127 ; RRID:Addgene_68127) were a gift from D. Conway, and mTFP1-Lifeact-7 was a gift from R. Campbell and M. Davidson (Addgene plasmid no. 54749; http://n2t.net/addgene:54749 ; RRID:Addgene_54749). ..

    Article Title: Reducing ER stress with chaperone therapy reverses sleep fragmentation and cognitive decline in aged mice
    Article Snippet: Data were scored with SleepSign Analysis Software (Kissei Comtec Co., LTD), and spectral data were analyzed as previously described (Franken et al., ; Hasan et al., ; Lim et al., ; Naidoo et al., ; Perron et al., ), with additional code generated by Dr. Isaac Perron (python, https://pypi.org/project/eeg‐sleep‐analysis/ ). .. Stereotaxic hippocampal injections were performed to deliver a BiP viral overexpression vector (Vector Biolabs; AAV5‐CamKIIa‐GRP78; titer 4.8 × 10 12 GC/ml) and an mCherry control vector (Addgene; pAAV5‐CaMKIIa‐mCherry; titer 2.3 × 10 −13 GC/ml) into the hippocampi of young and aged mice. ..



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    Image Search Results


    (A) mRNA expression of genes associated with cellular senescence measured by quantitative PCR in primary human renal proximal tubule epithelial cells (RPTECs) cultured with vehicle or cyclosporin A (CsA) for 24 h (n = 6 per group). (B) Western blot of p21 WAF1/Cip1 and actin in primary human RPTECs cultured with DMSO or CsA for 5 days. (C and D) Flow cytometric assay for quantification of senescence-associated β-galactosidase activity in primary human RPTECs cultured with DMSO, CsA or doxorubicin for 24 h (C). Mean fluorescence intensity is plotted in (D). RPTECs cultured with doxorubicin were used as a positive control. (E) Western blot of PDP1, PDHA1 phosphorylation at Ser293, total PDHA1 and actin in primary human RPTECs transduced with lentiviral vectors encoding the gene of either a control protein (mCherry) or PDP1 and cultured with DMSO or CsA for 12 h. PDHA1, pyruvate dehydrogenase E1 subunit alpha 1; PDP1, pyruvate dehydrogenase phosphatase 1. (F and G) Oxygen consumption rate (OCR) normalized to total protein levels in primary human RPTECs transduced with lentiviral vectors encoding the gene of either a control protein (mCherry) or PDP1 and cultured with DMSO or CsA for 24 h (F). Maximum mitochondrial respiration is calculated and plotted in (G). n = 15 per group. (H) mRNA expression of genes associated with cellular senescence measured by quantitative PCR in primary human RPTECs transduced with lentiviral vectors encoding the gene of either a control protein (mCherry) or PDP1 and cultured with DMSO or CsA for 24 h (n = 3 per group). (I) Schematic illustration of proposed mechanism of chronic calcineurin inhibitor nephrotoxicity. Inhibition of calcineurin causes deactivation of pyruvate dehydrogenase. This results in a decreased flux of TCA cycle metabolites and decreased mitochondrial respiration. Cellular metabolic dysfunction causes proinflammatory, profibrotic phenotypes associated with cellular senescence and induce kidney fibrosis. Data are presented as mean ± 1.96 SE (A, F and H) or mean ± SD (D and G). Unpaired two-tailed Welch’s t test was used. Holm’s correction for multiple comparisons was used in (G and H). n represents the number of biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001. n.s., not significant. See also Figure S4.

    Journal: bioRxiv

    Article Title: Calcineurin inhibition deactivates pyruvate dehydrogenase and induces proximal tubule cell metabolic dysfunction, causing profibrotic phenotype

    doi: 10.1101/2024.11.20.624584

    Figure Lengend Snippet: (A) mRNA expression of genes associated with cellular senescence measured by quantitative PCR in primary human renal proximal tubule epithelial cells (RPTECs) cultured with vehicle or cyclosporin A (CsA) for 24 h (n = 6 per group). (B) Western blot of p21 WAF1/Cip1 and actin in primary human RPTECs cultured with DMSO or CsA for 5 days. (C and D) Flow cytometric assay for quantification of senescence-associated β-galactosidase activity in primary human RPTECs cultured with DMSO, CsA or doxorubicin for 24 h (C). Mean fluorescence intensity is plotted in (D). RPTECs cultured with doxorubicin were used as a positive control. (E) Western blot of PDP1, PDHA1 phosphorylation at Ser293, total PDHA1 and actin in primary human RPTECs transduced with lentiviral vectors encoding the gene of either a control protein (mCherry) or PDP1 and cultured with DMSO or CsA for 12 h. PDHA1, pyruvate dehydrogenase E1 subunit alpha 1; PDP1, pyruvate dehydrogenase phosphatase 1. (F and G) Oxygen consumption rate (OCR) normalized to total protein levels in primary human RPTECs transduced with lentiviral vectors encoding the gene of either a control protein (mCherry) or PDP1 and cultured with DMSO or CsA for 24 h (F). Maximum mitochondrial respiration is calculated and plotted in (G). n = 15 per group. (H) mRNA expression of genes associated with cellular senescence measured by quantitative PCR in primary human RPTECs transduced with lentiviral vectors encoding the gene of either a control protein (mCherry) or PDP1 and cultured with DMSO or CsA for 24 h (n = 3 per group). (I) Schematic illustration of proposed mechanism of chronic calcineurin inhibitor nephrotoxicity. Inhibition of calcineurin causes deactivation of pyruvate dehydrogenase. This results in a decreased flux of TCA cycle metabolites and decreased mitochondrial respiration. Cellular metabolic dysfunction causes proinflammatory, profibrotic phenotypes associated with cellular senescence and induce kidney fibrosis. Data are presented as mean ± 1.96 SE (A, F and H) or mean ± SD (D and G). Unpaired two-tailed Welch’s t test was used. Holm’s correction for multiple comparisons was used in (G and H). n represents the number of biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001. n.s., not significant. See also Figure S4.

    Article Snippet: Lentiviral vectors that encoded PDP1 or a control protein (mCherry) were purchased from VectorBuilder Japan (Kanagawa, Japan).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Cell Culture, Western Blot, Flow Cytometry, Activity Assay, Fluorescence, Positive Control, Transduction, Control, Inhibition, Two Tailed Test