Journal: Frontiers in Pharmacology

Article Title: Periplanta americana extract regulates the Th17/Treg cell balance via Notch1 in ulcerative colitis

doi: 10.3389/fphar.2024.1534772

Figure Lengend Snippet: PAE inhibits the Notch1/Math1 pathway in UC mice. (A) Western blot images of Notch1 and Math1 in the colon. (B, C) Analysis of gray-scale of Notch1 and Math1 protein expression. (D) Immunohistochemistry images of Notch1 in colon (×100). (E) Immunohistochemistry images of Math1 in the colon (×100). (n = 3–6, * P < 0.05 and ** P < 0.01 versus control group, # P < 0.05 and ## P < 0.01 versus model group).

Article Snippet: After blocking with 5% skim milk for 1.5 h (h) at room temperature, the membranes were incubated overnight at 4°C with different primary antibodies against Occludin (13409-1-AP, Proteintech), Claudin 1 (13050-1-A, Proteintech), ZO1 (TA5145F, Abmart; 21773-1-A, Proteintech), Foxp3 (Ab215206, Abcam), RORγt (13205-1-AP, Proteintech), Math1 (21215-1-A, Proteintech), Notch1 (T55244S, Abmart; 20687-1-AP, Proteintech), Hes1(11988S, CST) and β-actin (ab179467; Abcam).

Techniques: Western Blot, Expressing, Immunohistochemistry, Control

Journal: Frontiers in Pharmacology

Article Title: Periplanta americana extract regulates the Th17/Treg cell balance via Notch1 in ulcerative colitis

doi: 10.3389/fphar.2024.1534772

Figure Lengend Snippet: PAE attenuates the disruption of tight junction proteins in HCoEpic cells by regulating activated Notch1-mediated Th17/Treg imbalance in vitro . (A) Effect of VPA on the viability of Jurkat T cells analyzed with CCK8 assay. (B) Effect of PAE on the viability of Jurkat cells analyzed with CCK-8 assay. (C) Western blot images of Notch1, Math1, RORγt, and Foxp3 in Jurkat T cells. (D–G) Analysis of gray-scale of Notch1, Math1, RORγt, and Foxp3 protein expression. (H) After notch1-activated Jurkat T cells were co-cultured with HCoEpic cells, Western blot images of occluding, claudin1, and ZO1 in HCoEpic cells. (I–K) Analysis of gray-scale of occluding, claudin1, and ZO1 protein expression. (n = 5–8, * P < 0.05 and ** P < 0.01 versus control group, # P < 0.05 and ## P < 0.01 versus VPA group).

Article Snippet: After blocking with 5% skim milk for 1.5 h (h) at room temperature, the membranes were incubated overnight at 4°C with different primary antibodies against Occludin (13409-1-AP, Proteintech), Claudin 1 (13050-1-A, Proteintech), ZO1 (TA5145F, Abmart; 21773-1-A, Proteintech), Foxp3 (Ab215206, Abcam), RORγt (13205-1-AP, Proteintech), Math1 (21215-1-A, Proteintech), Notch1 (T55244S, Abmart; 20687-1-AP, Proteintech), Hes1(11988S, CST) and β-actin (ab179467; Abcam).

Techniques: Disruption, In Vitro, CCK-8 Assay, Western Blot, Expressing, Cell Culture, Control