Journal: Synthetic and Systems Biotechnology

Article Title: XRE-type transcriptional regulator ProR controls prodigiosin synthesis in Serratia marcescens JNB5-1

doi: 10.1016/j.synbio.2026.02.014

Figure Lengend Snippet: ProR indirectly positively regulates the expression level of the prodigiosin-related pig gene cluster. (A) Growth curves of strains JNB5-1, SK6-61, SK6-61/pXW2010, SK6-61/pUCP18, Δ proR , Δ proR /pXW2010, and Δ proR /pUCP18. (B) Unit cell production of prodigiosin of strains JNB5-1, SK6-61, SK6-61/pXW2010, SK6-61/pUCP18, Δ proR , Δ proR /pXW2010, and Δ proR /pUCP18. (C) RT-qPCR analysis of the relative expression levels of the pigABCDEFGHIJKLMN genes in strains JNB5-1 and Δ proR . (D) β-galactosidase activity assay of strains SK68 and JNB5-1 carrying the P pigA - lacZ reporter gene. (E) Electrophoretic mobility shift assay (EMSA) demonstrating the binding capacity of ProR protein to the promoter region of the pig operon. Each reaction mixture contains 100 ng of PCR products. The protein concentrations are indicated above the lanes. (A to D) The experiment was performed independently three times. Error bars indicate standard deviations. Student's t-test was used to examine the mean differences between the data groups. ∗∗∗∗, P < 0.001.

Article Snippet: RT-qPCR analyses were performed on 200 ng/μL cDNA with ChamQ Universal SYBR qPCR mastermix kit (Vazyme) and primers from .

Techniques: Expressing, Quantitative RT-PCR, Activity Assay, Electrophoretic Mobility Shift Assay, Binding Assay

Journal: Poultry Science

Article Title: Transcriptomic insights into thermal manipulation and heat stress in skeletal muscles of broiler chickens

doi: 10.1016/j.psj.2026.106846

Figure Lengend Snippet: | Validation of selected differentially expressed genes using RT-qPCR. (A) Relative expression levels of COL1A1, HSP90AA1, HSPH1, FKBP5, MSMB, LECT2, and SFRP4, measured by RT-qPCR across all experimental groups. Fold changes were calculated using ΔCt values, then expressed relative to the thermal neutral control (Con-N, 2^−ΔΔCt). Data are presented as mean and SEM (standard error of the mean) in error bars ( p-value < 0.001 = ***, p-value < 0.01 = **, p-value < 0.05 = *).

Article Snippet: Briefly, cDNA was synthesized from 2 μg of total RNA using SuperScript IV VILO Master Mix (Invitrogen, Thermo Fisher Scientific, DE, USA) and diluted 1:400. qPCR was performed in duplicate using BlasTaqTM 2X qPCR MasterMix (Applied Biological Materials Inc., BC, Canada) on a Rotor-Gene Q MDx 5plex system (Qiagen, Germany) in 20 μL reactions containing 10.0 μL MasterMix, 0.5 μL of each primer (10 μM), 2.0 μL diluted cDNA, and nuclease-free water.

Techniques: Biomarker Discovery, Quantitative RT-PCR, Expressing, Control