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agena massarray methylation detection  (agena bioscience)


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    agena bioscience agena massarray methylation detection
    Agena Massarray Methylation Detection, supplied by agena bioscience, used in various techniques. Bioz Stars score: 96/100, based on 2219 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/massarray+methylation+detection/Methylation/ppr0552502-125-0-0
    Average 96 stars, based on 2219 article reviews
    agena massarray methylation detection - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Methylation:

    Article Title: Icariin Inhibits NF-κB Signaling in Asthmatic Lung Epithelium by Promoting Nrf2 Signaling.
    Article Snippet: esis. Query the CpGs sequence information of the target genes, and the primers were designed using EpiDesigner software, then DNA samples were processed with NaHSO3 for methylation detection using the Agena MassArray system: (1) Prepare PCR reaction reagents on ice. After amplification, take 3 μL of PCR product and mix it with 1 μL of 6× loading buffer, and use 1.5% agarose gel electrophoresis for

    Polymerase Chain Reaction:

    Article Title: Icariin Inhibits NF-κB Signaling in Asthmatic Lung Epithelium by Promoting Nrf2 Signaling.
    Article Snippet: esis. Query the CpGs sequence information of the target genes, and the primers were designed using EpiDesigner software, then DNA samples were processed with NaHSO3 for methylation detection using the Agena MassArray system: (1) Prepare PCR reaction reagents on ice. After amplification, take 3 μL of PCR product and mix it with 1 μL of 6× loading buffer, and use 1.5% agarose gel electrophoresis for

    Electrophoresis:

    Article Title: Icariin Inhibits NF-κB Signaling in Asthmatic Lung Epithelium by Promoting Nrf2 Signaling.
    Article Snippet: esis. Query the CpGs sequence information of the target genes, and the primers were designed using EpiDesigner software, then DNA samples were processed with NaHSO3 for methylation detection using the Agena MassArray system: (1) Prepare PCR reaction reagents on ice. After amplification, take 3 μL of PCR product and mix it with 1 μL of 6× loading buffer, and use 1.5% agarose gel electrophoresis for

    Sequencing:

    Article Title: Icariin Inhibits NF-κB Signaling in Asthmatic Lung Epithelium by Promoting Nrf2 Signaling.
    Article Snippet: esis. Query the CpGs sequence information of the target genes, and the primers were designed using EpiDesigner software, then DNA samples were processed with NaHSO3 for methylation detection using the Agena MassArray system: (1) Prepare PCR reaction reagents on ice. After amplification, take 3 μL of PCR product and mix it with 1 μL of 6× loading buffer, and use 1.5% agarose gel electrophoresis for

    Nucleic Acid Electrophoresis:

    Article Title: Icariin Inhibits NF-κB Signaling in Asthmatic Lung Epithelium by Promoting Nrf2 Signaling.
    Article Snippet: esis. Query the CpGs sequence information of the target genes, and the primers were designed using EpiDesigner software, then DNA samples were processed with NaHSO3 for methylation detection using the Agena MassArray system: (1) Prepare PCR reaction reagents on ice. After amplification, take 3 μL of PCR product and mix it with 1 μL of 6× loading buffer, and use 1.5% agarose gel electrophoresis for

    Amplification:

    Article Title: Icariin Inhibits NF-κB Signaling in Asthmatic Lung Epithelium by Promoting Nrf2 Signaling.
    Article Snippet: esis. Query the CpGs sequence information of the target genes, and the primers were designed using EpiDesigner software, then DNA samples were processed with NaHSO3 for methylation detection using the Agena MassArray system: (1) Prepare PCR reaction reagents on ice. After amplification, take 3 μL of PCR product and mix it with 1 μL of 6× loading buffer, and use 1.5% agarose gel electrophoresis for

    Agarose Gel Electrophoresis:

    Article Title: Icariin Inhibits NF-κB Signaling in Asthmatic Lung Epithelium by Promoting Nrf2 Signaling.
    Article Snippet: esis. Query the CpGs sequence information of the target genes, and the primers were designed using EpiDesigner software, then DNA samples were processed with NaHSO3 for methylation detection using the Agena MassArray system: (1) Prepare PCR reaction reagents on ice. After amplification, take 3 μL of PCR product and mix it with 1 μL of 6× loading buffer, and use 1.5% agarose gel electrophoresis for

    Software:

    Article Title: Icariin Inhibits NF-κB Signaling in Asthmatic Lung Epithelium by Promoting Nrf2 Signaling.
    Article Snippet: esis. Query the CpGs sequence information of the target genes, and the primers were designed using EpiDesigner software, then DNA samples were processed with NaHSO3 for methylation detection using the Agena MassArray system: (1) Prepare PCR reaction reagents on ice. After amplification, take 3 μL of PCR product and mix it with 1 μL of 6× loading buffer, and use 1.5% agarose gel electrophoresis for

    In Vitro:

    Article Title: Icariin Inhibits NF-κB Signaling in Asthmatic Lung Epithelium by Promoting Nrf2 Signaling.
    Article Snippet: esis. Query the CpGs sequence information of the target genes, and the primers were designed using EpiDesigner software, then DNA samples were processed with NaHSO3 for methylation detection using the Agena MassArray system: (1) Prepare PCR reaction reagents on ice. After amplification, take 3 μL of PCR product and mix it with 1 μL of 6× loading buffer, and use 1.5% agarose gel electrophoresis for

    Mass Spectrometry:

    Article Title: Icariin Inhibits NF-κB Signaling in Asthmatic Lung Epithelium by Promoting Nrf2 Signaling.
    Article Snippet: esis. Query the CpGs sequence information of the target genes, and the primers were designed using EpiDesigner software, then DNA samples were processed with NaHSO3 for methylation detection using the Agena MassArray system: (1) Prepare PCR reaction reagents on ice. After amplification, take 3 μL of PCR product and mix it with 1 μL of 6× loading buffer, and use 1.5% agarose gel electrophoresis for

    Modification:

    Article Title: Icariin Inhibits NF-κB Signaling in Asthmatic Lung Epithelium by Promoting Nrf2 Signaling.
    Article Snippet: esis. Query the CpGs sequence information of the target genes, and the primers were designed using EpiDesigner software, then DNA samples were processed with NaHSO3 for methylation detection using the Agena MassArray system: (1) Prepare PCR reaction reagents on ice. After amplification, take 3 μL of PCR product and mix it with 1 μL of 6× loading buffer, and use 1.5% agarose gel electrophoresis for

    Transmission Electron Microscopy:

    Article Title: Icariin Inhibits NF-κB Signaling in Asthmatic Lung Epithelium by Promoting Nrf2 Signaling.
    Article Snippet: esis. Query the CpGs sequence information of the target genes, and the primers were designed using EpiDesigner software, then DNA samples were processed with NaHSO3 for methylation detection using the Agena MassArray system: (1) Prepare PCR reaction reagents on ice. After amplification, take 3 μL of PCR product and mix it with 1 μL of 6× loading buffer, and use 1.5% agarose gel electrophoresis for

    Western Blot:

    Article Title: Icariin Inhibits NF-κB Signaling in Asthmatic Lung Epithelium by Promoting Nrf2 Signaling.
    Article Snippet: esis. Query the CpGs sequence information of the target genes, and the primers were designed using EpiDesigner software, then DNA samples were processed with NaHSO3 for methylation detection using the Agena MassArray system: (1) Prepare PCR reaction reagents on ice. After amplification, take 3 μL of PCR product and mix it with 1 μL of 6× loading buffer, and use 1.5% agarose gel electrophoresis for

    In Vivo:

    Article Title: Icariin Inhibits NF-κB Signaling in Asthmatic Lung Epithelium by Promoting Nrf2 Signaling.
    Article Snippet: esis. Query the CpGs sequence information of the target genes, and the primers were designed using EpiDesigner software, then DNA samples were processed with NaHSO3 for methylation detection using the Agena MassArray system: (1) Prepare PCR reaction reagents on ice. After amplification, take 3 μL of PCR product and mix it with 1 μL of 6× loading buffer, and use 1.5% agarose gel electrophoresis for

    Mouse Assay:

    Article Title: Icariin Inhibits NF-κB Signaling in Asthmatic Lung Epithelium by Promoting Nrf2 Signaling.
    Article Snippet: esis. Query the CpGs sequence information of the target genes, and the primers were designed using EpiDesigner software, then DNA samples were processed with NaHSO3 for methylation detection using the Agena MassArray system: (1) Prepare PCR reaction reagents on ice. After amplification, take 3 μL of PCR product and mix it with 1 μL of 6× loading buffer, and use 1.5% agarose gel electrophoresis for

    Enzyme-linked Immunosorbent Assay:

    Article Title: Icariin Inhibits NF-κB Signaling in Asthmatic Lung Epithelium by Promoting Nrf2 Signaling.
    Article Snippet: esis. Query the CpGs sequence information of the target genes, and the primers were designed using EpiDesigner software, then DNA samples were processed with NaHSO3 for methylation detection using the Agena MassArray system: (1) Prepare PCR reaction reagents on ice. After amplification, take 3 μL of PCR product and mix it with 1 μL of 6× loading buffer, and use 1.5% agarose gel electrophoresis for

    DNA Methylation Assay:

    Article Title: Icariin Inhibits NF-κB Signaling in Asthmatic Lung Epithelium by Promoting Nrf2 Signaling.
    Article Snippet: esis. Query the CpGs sequence information of the target genes, and the primers were designed using EpiDesigner software, then DNA samples were processed with NaHSO3 for methylation detection using the Agena MassArray system: (1) Prepare PCR reaction reagents on ice. After amplification, take 3 μL of PCR product and mix it with 1 μL of 6× loading buffer, and use 1.5% agarose gel electrophoresis for



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    A A negative correlation between ZNF334 <t>methylation</t> level and its mRNA expression (Pearson r = −0.53, R 2 = 0.28) B mRNA expression of ZNF334 in cancer and adjacent tissues of 213 liver cancer patients. C Western-blot diagram of ZNF334 expression in cancer and adjacent tissues of 12 liver cancer patients. D Methylation mass spectrum primers of ZNF334 promoter region and mass spectrometry detection product sequence (green indicates the EPIC chip probe cg07139762 site, which is in the ZNF334 promoter region; yellow indicates the <t>MassARRAY</t> methylation mass spectrometry detection sites, a total of 29). E Heat map of methylation level of ZNF334 promoter region in cancer and adjacent tissues of 25 patients with liver cancer. F The ratio of methylation degree of ZNF334 promoter in cancer and adjacent tissues (T/P) of 25 patients with liver cancer. G The average methylation degree of ZNF334 promoter in cancer and adjacent tissues of 25 patients with liver cancer. **** p < 0.0001.
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    Image Search Results


    A A negative correlation between ZNF334 methylation level and its mRNA expression (Pearson r = −0.53, R 2 = 0.28) B mRNA expression of ZNF334 in cancer and adjacent tissues of 213 liver cancer patients. C Western-blot diagram of ZNF334 expression in cancer and adjacent tissues of 12 liver cancer patients. D Methylation mass spectrum primers of ZNF334 promoter region and mass spectrometry detection product sequence (green indicates the EPIC chip probe cg07139762 site, which is in the ZNF334 promoter region; yellow indicates the MassARRAY methylation mass spectrometry detection sites, a total of 29). E Heat map of methylation level of ZNF334 promoter region in cancer and adjacent tissues of 25 patients with liver cancer. F The ratio of methylation degree of ZNF334 promoter in cancer and adjacent tissues (T/P) of 25 patients with liver cancer. G The average methylation degree of ZNF334 promoter in cancer and adjacent tissues of 25 patients with liver cancer. **** p < 0.0001.

    Journal: Cell Death & Disease

    Article Title: DNA hypermethylation modification promotes the development of hepatocellular carcinoma by depressing the tumor suppressor gene ZNF334

    doi: 10.1038/s41419-022-04895-6

    Figure Lengend Snippet: A A negative correlation between ZNF334 methylation level and its mRNA expression (Pearson r = −0.53, R 2 = 0.28) B mRNA expression of ZNF334 in cancer and adjacent tissues of 213 liver cancer patients. C Western-blot diagram of ZNF334 expression in cancer and adjacent tissues of 12 liver cancer patients. D Methylation mass spectrum primers of ZNF334 promoter region and mass spectrometry detection product sequence (green indicates the EPIC chip probe cg07139762 site, which is in the ZNF334 promoter region; yellow indicates the MassARRAY methylation mass spectrometry detection sites, a total of 29). E Heat map of methylation level of ZNF334 promoter region in cancer and adjacent tissues of 25 patients with liver cancer. F The ratio of methylation degree of ZNF334 promoter in cancer and adjacent tissues (T/P) of 25 patients with liver cancer. G The average methylation degree of ZNF334 promoter in cancer and adjacent tissues of 25 patients with liver cancer. **** p < 0.0001.

    Article Snippet: MassARRAY methylation detection technology (Sequenom, USA) performed by CapitalBio Technology (Shanghai, China) was used to detect the methylation level of ZNF334 promoter (human) in carcinoma and paracancerous tissues of 25 HCC patients and Zfp334 promoter (mice) in treatment and control group.

    Techniques: Methylation, Expressing, Western Blot, Mass Spectrometry, Sequencing