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( A ) Schematic depiction of miR- 200b-a-429 and miR-200c-141 genomic loci showing CpG islands (green), putative transcription start sites (TSS) and miRNA stem-loop sequences (red). The regions analyzed for DNA methylation <t>(MassArray)</t> are indicated by a black bar. Chromosomal location is indicated between brackets. ( B ) The DNA methylation levels across the regions shown in panel ( A ) were quantified in breast cancers by Sequenom MassArray® <t>MALDI-TOF</t> platform. The mean percentage of methylation levels in three tumor types (ER+, TN, MBC) are represented as box-plots. Statistical significance was determined by Student’s t test. ( C ) Expression and %CpG methylation data are represented on a scatter plot matrix showing correlation between the indicated variables, a 95% bivariate normal density ellipse is imposed on each scatterplot. Pearson correlation coefficients (R) and significance probabilities (P) are shown. Statistically significant p values (<0.05) are highlighted in red.
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( A ) Schematic depiction of miR- 200b-a-429 and miR-200c-141 genomic loci showing CpG islands (green), putative transcription start sites (TSS) and miRNA stem-loop sequences (red). The regions analyzed for DNA methylation <t>(MassArray)</t> are indicated by a black bar. Chromosomal location is indicated between brackets. ( B ) The DNA methylation levels across the regions shown in panel ( A ) were quantified in breast cancers by Sequenom MassArray® <t>MALDI-TOF</t> platform. The mean percentage of methylation levels in three tumor types (ER+, TN, MBC) are represented as box-plots. Statistical significance was determined by Student’s t test. ( C ) Expression and %CpG methylation data are represented on a scatter plot matrix showing correlation between the indicated variables, a 95% bivariate normal density ellipse is imposed on each scatterplot. Pearson correlation coefficients (R) and significance probabilities (P) are shown. Statistically significant p values (<0.05) are highlighted in red.
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( A ) Schematic depiction of miR- 200b-a-429 and miR-200c-141 genomic loci showing CpG islands (green), putative transcription start sites (TSS) and miRNA stem-loop sequences (red). The regions analyzed for DNA methylation <t>(MassArray)</t> are indicated by a black bar. Chromosomal location is indicated between brackets. ( B ) The DNA methylation levels across the regions shown in panel ( A ) were quantified in breast cancers by Sequenom MassArray® <t>MALDI-TOF</t> platform. The mean percentage of methylation levels in three tumor types (ER+, TN, MBC) are represented as box-plots. Statistical significance was determined by Student’s t test. ( C ) Expression and %CpG methylation data are represented on a scatter plot matrix showing correlation between the indicated variables, a 95% bivariate normal density ellipse is imposed on each scatterplot. Pearson correlation coefficients (R) and significance probabilities (P) are shown. Statistically significant p values (<0.05) are highlighted in red.
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Image Search Results


( A ) Schematic depiction of miR- 200b-a-429 and miR-200c-141 genomic loci showing CpG islands (green), putative transcription start sites (TSS) and miRNA stem-loop sequences (red). The regions analyzed for DNA methylation (MassArray) are indicated by a black bar. Chromosomal location is indicated between brackets. ( B ) The DNA methylation levels across the regions shown in panel ( A ) were quantified in breast cancers by Sequenom MassArray® MALDI-TOF platform. The mean percentage of methylation levels in three tumor types (ER+, TN, MBC) are represented as box-plots. Statistical significance was determined by Student’s t test. ( C ) Expression and %CpG methylation data are represented on a scatter plot matrix showing correlation between the indicated variables, a 95% bivariate normal density ellipse is imposed on each scatterplot. Pearson correlation coefficients (R) and significance probabilities (P) are shown. Statistically significant p values (<0.05) are highlighted in red.

Journal: PLoS ONE

Article Title: MicroRNA-200 Family Modulation in Distinct Breast Cancer Phenotypes

doi: 10.1371/journal.pone.0047709

Figure Lengend Snippet: ( A ) Schematic depiction of miR- 200b-a-429 and miR-200c-141 genomic loci showing CpG islands (green), putative transcription start sites (TSS) and miRNA stem-loop sequences (red). The regions analyzed for DNA methylation (MassArray) are indicated by a black bar. Chromosomal location is indicated between brackets. ( B ) The DNA methylation levels across the regions shown in panel ( A ) were quantified in breast cancers by Sequenom MassArray® MALDI-TOF platform. The mean percentage of methylation levels in three tumor types (ER+, TN, MBC) are represented as box-plots. Statistical significance was determined by Student’s t test. ( C ) Expression and %CpG methylation data are represented on a scatter plot matrix showing correlation between the indicated variables, a 95% bivariate normal density ellipse is imposed on each scatterplot. Pearson correlation coefficients (R) and significance probabilities (P) are shown. Statistically significant p values (<0.05) are highlighted in red.

Article Snippet: The DNA methylation status of miR-200f loci in tumor samples were inspected using Sequenom MassArray® MALDI-TOF platform.

Techniques: DNA Methylation Assay, Methylation, Expressing, CpG Methylation Assay