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massarray iplextm gold genotyping platform  (Sequenom)
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Sequencing traces of NESP55 gene for genomic DNA and cDNA from both maternal and foetal tissues . The genomic DNA from the mother of each foetus allowed <t>genotyping</t> of the maternal genotype, while the genomic DNA from each foetus allowed genotyping of the each foetus. Arrows indicate the exonic <t>SNP,</t> denoted R (A/G), which was used for both DNA genotyping and to analyse allele-specific expression status of NESP55 transcripts (cDNA) across tissues from 8 and 10 weeks old foetuses. Column number 4 presents the genotypes for all samples/animals tested. Column 5 indicates whether monoallelic ( i.e . uniparental) expression was detected.
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Sequencing traces of NESP55 gene for genomic DNA and cDNA from both maternal and foetal tissues . The genomic DNA from the mother of each foetus allowed genotyping of the maternal genotype, while the genomic DNA from each foetus allowed genotyping of the each foetus. Arrows indicate the exonic SNP, denoted R (A/G), which was used for both DNA genotyping and to analyse allele-specific expression status of NESP55 transcripts (cDNA) across tissues from 8 and 10 weeks old foetuses. Column number 4 presents the genotypes for all samples/animals tested. Column 5 indicates whether monoallelic ( i.e . uniparental) expression was detected.

Journal: BMC Genetics

Article Title: DNA sequence polymorphisms within the bovine guanine nucleotide-binding protein Gs subunit alpha (Gsα)-encoding ( GNAS ) genomic imprinting domain are associated with performance traits

doi: 10.1186/1471-2156-12-4

Figure Lengend Snippet: Sequencing traces of NESP55 gene for genomic DNA and cDNA from both maternal and foetal tissues . The genomic DNA from the mother of each foetus allowed genotyping of the maternal genotype, while the genomic DNA from each foetus allowed genotyping of the each foetus. Arrows indicate the exonic SNP, denoted R (A/G), which was used for both DNA genotyping and to analyse allele-specific expression status of NESP55 transcripts (cDNA) across tissues from 8 and 10 weeks old foetuses. Column number 4 presents the genotypes for all samples/animals tested. Column 5 indicates whether monoallelic ( i.e . uniparental) expression was detected.

Article Snippet: All SNP genotyping was performed commercially by Sequenom Inc. (San Diego, CA, USA; http://www.sequenom.com using their proprietary MassARRAY iPLEXTM Gold genotyping platform.

Techniques: Sequencing, Expressing