Journal: Bioactive Materials

Article Title: A cell motility-based selective hydrogel enables rapid generation of nerve-repairing blood clots

doi: 10.1016/j.bioactmat.2026.05.015

Figure Lengend Snippet: Effect and mechanism of the nano-gelatin on NSCs . (A) A diagram of the layered composite structure of the nano-gelatin after hemostasis. (B) Representative SEM images showing the platelet-derived extracellular vesicles in the nano-gelatin after hemostasis. (C) ALB contents in the cryogels following hemostasis (N = 4). (D) NGF contents in the cryogels (N = 3). (E) SDF-1 contents in the cryogels (N = 3). (F) Representative images showing NSCs migrating through the Transwell membrane into the plate with cryogels. (G) Quantification of the NSC numbers that migrated through the Transwell membrane into the plate (N = 4). (H) Representative images of the live/dead staining showing the survival and morphology of NSCs on the cryogels. (I) Cytotoxicity of the cryogels on NSCs by CCK-8 assay. (J) Representative images of immunostaining against F-actin, paxillin, and vinculin for cells encapsulated in the nano-gelatin and the GelMA hydrogel. (K) Representative images of immunostaining against Tuj-1 and GFAP. (L) Volcano plot analyzing DEGs between the nano-gelatin group and the control group. (M) The enriched GO pathways. (N) The enriched KEGG pathways. (O) The heatmaps of DEGs associated with Focal adhesion. (P) Schematic diagram of the potential mechanism by which the nano-gelatin regulates NSC migration and differentiation to promote nerve repair. Statistical analysis was performed using one-way ANOVA followed by Tukey's multiple comparisons test.

Article Snippet: Then, the samples were fixed and stained with astrocyte marker GFAP (1:500, CST, Rabbit mAb #80788) and neuron marker Tuj-1 (1:200, HUABIO, SP06-00) to assess differentiation.

Techniques: Derivative Assay, Membrane, Staining, CCK-8 Assay, Immunostaining, Control, Migration

Journal: Bioactive Materials

Article Title: ADGRG1-targeted hypoxia preconditioned extracellular vesicles ameliorate intervertebral disc degeneration by delivering taurine to disrupt the oxidative stress feedback loop-driven ferroptosis in nucleus pulposus cells

doi: 10.1016/j.bioactmat.2026.02.029

Figure Lengend Snippet: Preparation and characterization of engineered ADGRG1-targeting and hypoxia-treated EVs. (A)Induced fit docking analysis of ADGRG1-binding peptide (A1TP, 7 peptides) and extracellular domain of ADGRG1 protein (PDB database: 7SF8). (B) Analysis of the binding of the A1TP to purified ADGRG1 proteins using a microscale thermophoresis (MST) binding assay. (C) Induced fit docking analysis of A1TP-PEG and extracellular domain of ADGRG1 protein. (D) The binding free energy between A1TP or A1TP-PEG and ADGRG1 were calculated using molecular dynamics simulations. Lower values indicate more stable interactions, with values less than or equal to −20 considered as stable binding modes. (E) Schematic illustration of the conjugating reaction between DSPE-PEG-Alkyne and A1TP. Schematic illustration of the fabrication of A1TP-HX-EVs through external modification by A1TP anchoring. Specific steps for the synthesis of DSPE-PEG-A1TP (DPA) are shown in . (F) FT-IR analysis showed the characteristic peaks of the DSPE-PEG-A1TP. The new triazole ring itself showed a characteristic C=N stretching vibration, a peak at 1538 cm −1 revealed the successful conjugation of A1TP. (G) H Nuclear magnetic resonance (NMR) spectra of DSPE-PEG-A1TP in D2O. The hydrogen signatures of the phenyl and phenol groups at 7.5-8.0 ppm confirmed the successful conjugation of DSPE to A1TP. (H) Western blot analysis verified the presence of three EV marker proteins (ALIX, TSG101, and CD81) and one EV negative marker (GM130) in EVs, HX-EVs, and A1TP-HX-EVs. (I) Transmission electron microscopy (TEM) images of EVs, HX-EVs and A1TP-HX-EVs. Scale bar, 200 nm. (J) Zeta potentials of EVs, HX-EVs and A1TP-HX-EVs, n = 3. Two-tailed unpaired Student's t-test was used for statistical analysis. ns, not significant. A two-tailed unpaired Student's t-test was used for statistical analysis. (K) Representative images of the spherical morphology and dispersion states of EVs, HX-EVs and A1TP-HX-EVs. Scale bar, 500 nm. (L) Size distributions of EVs, HX-EVs and A1TP-HX-EVs.

Article Snippet: Finally, the presence of the characteristic EV markers Alix (92880, Cell Signaling Technology), CD81 (56039, Cell Signaling Technology) and TSG101 (sc-7964, Santa Cruz Biotechnology) was confirmed by Western blot analysis.

Techniques: Binding Assay, Purification, Microscale Thermophoresis, Modification, Conjugation Assay, Nuclear Magnetic Resonance, Western Blot, Marker, Transmission Assay, Electron Microscopy, Two Tailed Test, Dispersion

Journal: iScience

Article Title: Prevalence of ARGs in poultry-associated E. coli in Zhejiang Province, China: A genotypic survey

doi: 10.1016/j.isci.2026.116177

Figure Lengend Snippet: PCR-based antibiotic resistance gene fingerprint patterns of E. coli strain isolated from sample P60 The figure displays amplification results for 92 genetic markers associated with AMR. Each lane represents a specific PCR target. 1: β-lactam TEM group. 2–5: β-lactam CTX-M group. 6–10: β-lactam ACC, DHA, FOX, VEB, SHV groups. 11–12: β-lactam CMY group. 13–19: β-lactam IMP, SMP, VIM, OXA, BIC, KPC, NDM groups. 20–21: chloramphenicol ( cmlA , catA1 ). 22–26: colistin ( mcr -1, mcr -2, mcr -3, mcr -4, mcr -5). 27: florfenicol ( floR ). 28–30: gentamicin ( aac(3)-IV , ant(2″)-I , aac(3)-II ). 31: linezolid ( optrA ). 32–34: neomycin ( aph(3′)-III-I variants). 35–45: quinolones ( gyrA Salmonella , parC Salmonella , gyrA E. coli , parC E. coli , qnrA , qnrB , qnrC , qnrD , qnrS , aac(6′)-Ib-cr , qepA ). 46–49: streptomycin ( strA , strB , aadA2 , aadE ). 50–52: sulfamethoxazole ( sul1 , sul2 , sul3 ). 53–72: tetracyclines ( tetA , tetB , tetC , tetD , tetE , tetG , tetH , tetK , tetL , tetM , tetO , tetS , tetT , tetW , tetZ , tet31 , tet32 , tet33 , tet34 , tet39 ). 73–75: glycopeptides ( vanA , vanB , vanX ). 76–80: macrolides ( ermA , ermB , ermC , ermF ). 81–88: streptogramins ( vat , vatB , vatD , vatE , vgaA , vgaB , vgbA , vgbB ). 89–92: MRSA-associated markers ( spa , mecA , mecC , pvl ). M: DNA ladder.

Article Snippet: DNA Marker (200∼2000 bp) , BBI , Cat#B600339.

Techniques: Isolation, Amplification