Journal: Frontiers in Cellular Neuroscience

Article Title: Neuroimmune pathways involvement in neurodegeneration of R6/2 mouse model of Huntington’s disease

doi: 10.3389/fncel.2024.1360066

Figure Lengend Snippet: CD47 expression in striatal neurons of R6/2 mice. Confocal laser scanning microscopy images of double-label immunofluorescence for CD47 (visualized in red fluorescence) and the Nissl-like fluorescent marker Neurotrace (visualized in green fluorescence). (A–D) Images show the expression pattern of CD47 in the striatum of 5 and 13 weeks-old wild-type mice and in 5 and 13 weeks-old R6/2 mice. (E,F) Detail of cytoplasmic membrane CD47 expression in 13 weeks-old Wt mice. In 13 weeks-old R6/2 mice CD47 is lower due to cell death process (G) Histogram shows CD47 intensity quantification which is significantly decreased in the striatal neurons of 13 weeks-old R6/2 mice [Genotype effect F (1,153) = 11.97, *** P = 0.0007]. (H,I) Tukey’s multiple comparisons analysis revealed a significant protein decrease of striatal neuronal CD47, as evaluated by western blotting in R6/2 mice [Genotype effect F (1,20) = 8.667, ** P = 0.0080; Time effect F (1,20) = 5.802, ** P = 0.0258; Genotype X Time Interaction F (1,20) = 37.89, *** P < 0,0001].

Article Snippet: Coronal brain sections were incubated with the marker CD47 (BS-21460R, rabbit polyclonal anti-CD47, Bioss, USA) at 1:200 dilution in a 0.1 M PB solution containing 0.3% Triton X-100 for 72 h at 4°C.

Techniques: Expressing, Confocal Laser Scanning Microscopy, Immunofluorescence, Fluorescence, Marker, Membrane, Western Blot

Journal: Turkish Journal of Medical Sciences

Article Title: Does CD47 expression have prognostic significance in classical Hodgkin lymphoma?

doi: 10.55730/1300-0144.5958

Figure Lengend Snippet: A. Strong membranous staining is observed in HRS cells using CD47 immunohistochemistry at 200× magnification. The staining highlights the robust expression of CD47 on the cell membrane, indicative of its significant presence on these malignant cells, B: A dot-like staining pattern is visible in the Golgi zone of a subset of HRS cells at 400× magnification, C: Nuclear positivity is widespread in HRS cells, as detected by Epstein–Barr virus-encoded RNA (EBER) in situ hybridization (ISH) at 200× magnification.

Article Snippet: All cases were stained using the CD47 immunohistochemistry marker (mouse monoclonal B6H12, catalog no: 14-0479-82, Invitrogen Thermo Fisher Scientific, 1:100 dilution).

Techniques: Staining, Immunohistochemistry, Expressing, Membrane, Virus, In Situ Hybridization

Journal: Turkish Journal of Medical Sciences

Article Title: Does CD47 expression have prognostic significance in classical Hodgkin lymphoma?

doi: 10.55730/1300-0144.5958

Figure Lengend Snippet: Comparison of CD47 staining score with IPI. The scatterplot shows the distribution of International Prognostic Index (IPI) scores as a function of CD47 expression (%). Each red cross represents an individual data point. The blue contour shading represents kernel density estimation (KDE), highlighting the concentration of data points within the sample population. This visualization demonstrates the overall distribution and density of the data points, suggesting a potential trend or relationship between CD47 expression and IPI score.

Article Snippet: All cases were stained using the CD47 immunohistochemistry marker (mouse monoclonal B6H12, catalog no: 14-0479-82, Invitrogen Thermo Fisher Scientific, 1:100 dilution).

Techniques: Comparison, Staining, Expressing, Concentration Assay

Journal: Turkish Journal of Medical Sciences

Article Title: Does CD47 expression have prognostic significance in classical Hodgkin lymphoma?

doi: 10.55730/1300-0144.5958

Figure Lengend Snippet: The distribution of age, sex, IPI, EBER, and marker results according to CD47 coding for the 120 patients evaluated in the study.

Article Snippet: All cases were stained using the CD47 immunohistochemistry marker (mouse monoclonal B6H12, catalog no: 14-0479-82, Invitrogen Thermo Fisher Scientific, 1:100 dilution).

Techniques: Marker, Comparison

Journal: Nanoscale Advances

Article Title: A comprehensive review of challenges and advances in exosome-based drug delivery systems

doi: 10.1039/d4na00501e

Figure Lengend Snippet: Hybrid exosome-based drug delivery systems representing the source, synthetic carrier, hybridization technique, and outcomes a

Article Snippet: These hybrid exosomes were shown to have individual cell specific protein markers of RBC (CD47) and MCF-7 (EpCAM, galectin 3, and cadherin) and were loaded with DOX.

Techniques: Hybridization, Marker, Liposomes, Encapsulation, Incubation, Transfection, Isolation, Expressing, Plasmid Preparation, CRISPR, In Vivo, Control, Suspension, Membrane, Derivative Assay, Clinical Proteomics, Imaging, Binding Assay, Labeling, Fluorescence, MTT Assay