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Journal: eLife
Article Title: Human receptive endometrial assembloid for deciphering the implantation window
doi: 10.7554/eLife.90729
Figure Lengend Snippet: ( A ) Human endometrial assembloids constructed from adult stem cells were treated with expansion medium (ExM) (CTRL) or subjected to hormonal stimulation. Timeline of endometrial assembloid cultured by ExM (CTRL), ovarian steroid hormones simulating secretory phase (SEC), ovarian steroid hormones combining PRL and placental hormones to mimic the window of implantation (WOI). ( B ) Endometrial assembloids from the CTRL, SEC, and WOI groups, which were subjected to hormone treatment on Days 0, 2, and 8, exhibited comparable growth patterns throughout the culture period. Scale bar = 200 μm. ( C ) The dynamic changes of the counts of assembloids over time in each hormone regimen. ( D ) The dynamic changes of the area of assembloids over time in each hormone regimen. ( E ) Heatmap showing receptivity-related gene expression profile of assembloids in each hormone regimen. The color represents log-transformed fold change of gene expression. ( F ) Validation of receptivity markers (IGFBP1, MAOA, and DPP4) with immunofluorescence (IF) in the CTRL, SEC, and WOI endometrial assembloids in vitro. Nuclei were counterstained with DAPI. Scale bar = 30 μm. The bar chart displays the quantitative comparison of receptivity markers among three groups. * p ≤0.05, ** p ≤0.005, *** p ≤0.0005, **** p ≤0.0001.n=4 (CTRL) and 5 (SEC and WOI) (IGFBP1), n=4 (MAO-A and DPP4).
Article Snippet: Antibody ,
Techniques: Construct, Cell Culture, Gene Expression, Transformation Assay, Biomarker Discovery, Immunofluorescence, In Vitro, Comparison