Journal: Materials Today Bio

Article Title: Antithrombotic and antibacterial surface coating based on spiky silver nanoparticles: A counterattack against clotting and biofilm

doi: 10.1016/j.mtbio.2026.102762

Figure Lengend Snippet: In vitro biocompatibility of surface coatings. (A) ROS generation levels of macrophages cultured on the surface coatings. (B) Proliferation of SVEC4-10 and RAW 264.7 cells on the surfaces and (C) representative images illustrating cell morphology using LIVE/DEAD™ reagents. (D) Images and (E) haemolytic activity of surface coatings. Statistical comparisons of samples with the control were performed using one-way and two-way ANOVA tests.

Article Snippet: The cytocompatibility of the AgIONPs coatings was assessed using the LIVE/DEADTM cell viability assay, employing SVEC4-10 endothelial cells (CRL-2181, passage 10) and RAW 264.7 macrophages (ATCC TIB-71, passage 21) as representative in vitro models.

Techniques: In Vitro, Cell Culture, Activity Assay, Control

Journal: Materials Today Bio

Article Title: Injectable chitosan-based hydrogel via in situ gelation modulates the inflammatory microenvironment and facilitates minimally invasive repair of peripheral nerve injury

doi: 10.1016/j.mtbio.2026.102814

Figure Lengend Snippet: Anti-inflammatory and antioxidative capacity of hydrogels. Flow cytometry (A) reveals that IBU-CS-GP hydrogel significantly promote M2 polarization of LPS-stimulated RAW264.7 macrophages (B) RT-PCR analysis suggests that IBU-CS-GP downregulates pro-inflammatory gene TNF-α (C) and upregulates anti-inflammatory genes of IL-4 (D) in comparison to CS-MA and CS-GP. IBU-CS-GP markedly attenuates ROS and NO production in LPS-stimulated macrophages, as indicated by DCFH staining (H), flow cytometry analysis (G), and ROS & NO quantification (E–F). One-way ANOVA with Tukey's post hoc test and n = 3 for B-F; ∗∗p < 0.01, ∗∗∗p < 0.001, n.s. ANOVA, analysis of variance; CS, chitosan; IBU, ibuprofen; GP, genipin; MA, methacrylic anhydride; LPS, lipopolysaccharides; n.s., not significant; NO, nitric oxide; PCR, polymerase chain reaction; ROS, reactive oxygen species; DCFH, 2′,7′-Dichlorodihydrofluorescein; DAPI, 4′,6-diamidino-2-phenylindole.

Article Snippet: Mouse fibroblasts (L929, ATCC), human umbilical vein endothelial cells (HUVECs, ATCC), and mouse macrophages (RAW 264.7, ATCC) were provided by the Cell Bank of the Chinese Academy of Sciences.

Techniques: Flow Cytometry, Reverse Transcription Polymerase Chain Reaction, Comparison, Staining, Polymerase Chain Reaction

Journal: Materials Today Bio

Article Title: Injectable chitosan-based hydrogel via in situ gelation modulates the inflammatory microenvironment and facilitates minimally invasive repair of peripheral nerve injury

doi: 10.1016/j.mtbio.2026.102814

Figure Lengend Snippet: Pro-angiogenic and pro-migratory effects of hydrogels. Immunofluorescence staining shows blood vessel formation of HUVECs in LPS-macrophage condition medium (A). Scratch assays and Transwell migration assays of HUVECs (B) and L929 cells (C). Quantification of junctions (D), branches (E), wound closure percentage (F–G) and migrated cells (H–I). One-way ANOVA with Tukey's post hoc test and n = 3 for D-I; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, n.s. ANOVA, analysis of variance; CS, chitosan; IBU, ibuprofen; GP, genipin; MA, methacrylic anhydride; LPS, lipopolysaccharides; HUVEC, human umbilical vein endothelial cell; n.s., not significant.

Article Snippet: Mouse fibroblasts (L929, ATCC), human umbilical vein endothelial cells (HUVECs, ATCC), and mouse macrophages (RAW 264.7, ATCC) were provided by the Cell Bank of the Chinese Academy of Sciences.

Techniques: Immunofluorescence, Staining, Migration

Journal: Materials Today Bio

Article Title: Antithrombotic and antibacterial surface coating based on spiky silver nanoparticles: A counterattack against clotting and biofilm

doi: 10.1016/j.mtbio.2026.102762

Figure Lengend Snippet: In vitro biocompatibility of surface coatings. (A) ROS generation levels of macrophages cultured on the surface coatings. (B) Proliferation of SVEC4-10 and RAW 264.7 cells on the surfaces and (C) representative images illustrating cell morphology using LIVE/DEAD™ reagents. (D) Images and (E) haemolytic activity of surface coatings. Statistical comparisons of samples with the control were performed using one-way and two-way ANOVA tests.

Article Snippet: The cytocompatibility of the AgIONPs coatings was assessed using the LIVE/DEADTM cell viability assay, employing SVEC4-10 endothelial cells (CRL-2181, passage 10) and RAW 264.7 macrophages (ATCC TIB-71, passage 21) as representative in vitro models.

Techniques: In Vitro, Cell Culture, Activity Assay, Control

Journal: Materials Today Bio

Article Title: Immunomodulatory and dentinogenic potential of surface-engineered hesperetin-functionalized composite scaffolds for pulp-dentin regeneration

doi: 10.1016/j.mtbio.2026.102930

Figure Lengend Snippet: Indirect interaction between scaffolds and inflammatory cells. RAW 264.7 murine macrophages were previously stimulated (positive control, PC) or not (negative control, NC) with LPS (100 ng/mL), followed by treatment with 24-h extracts from each scaffold. (A) Cell metabolism (alamarBlue assay) of macrophages after 24-h exposure to scaffold extracts. Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc test ( p < 0.05). (B-D) Relative gene expression of Tnf , Il1b , and Nos2 after 3-h exposure to the extracts. Statistical analysis was performed using Welch's ANOVA followed by the Games-Howell post hoc test ( p < 0.05). (E-G) Secreted TNF-α, IL-1β, and nitrite levels in macrophage culture supernatants after 24-h exposure to the extracts. Statistical analysis was performed using Welch's ANOVA followed by the Games-Howell post hoc test ( p < 0.05). All data are presented as mean ± SD (n = 8). The asterisk indicates no detectable protein in the NC group extract.

Article Snippet: RAW 264.7 murine macrophages (TIB-71, ATCC, Rockville, MD, USA) were cultured in high-glucose Dulbecco's modified Eagle's medium (DMEM; GIBCO) supplemented with 10% FBS (GIBCO) and 1% penicillin/streptomycin (GIBCO) at 37 °C in 5% CO 2 .

Techniques: Positive Control, Negative Control, Alamar Blue Assay, Gene Expression