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Assessment of rate of killing. Synchronous cultures were subjected to 3 x EC 50 concentrations of <t>macrocyclic</t> compounds or dihydroartemisinin (DHA) for 6 or 12 h, followed by washing to remove the inhibitor and incubating in the growth medium in the absence of compounds to monitor recovery. (A) Compounds added at the schizont stage, (B) treatment at the trophozoite stage, and (C) ring stage culture exposed to the compound. Compounds were added at a stage where they exhibit block in cell cycle progression.
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Assessment of rate of killing. Synchronous cultures were subjected to 3 x EC 50 concentrations of macrocyclic compounds or dihydroartemisinin (DHA) for 6 or 12 h, followed by washing to remove the inhibitor and incubating in the growth medium in the absence of compounds to monitor recovery. (A) Compounds added at the schizont stage, (B) treatment at the trophozoite stage, and (C) ring stage culture exposed to the compound. Compounds were added at a stage where they exhibit block in cell cycle progression.

Journal: Frontiers in Pharmacology

Article Title: DeepMalaria: Artificial Intelligence Driven Discovery of Potent Antiplasmodials

doi: 10.3389/fphar.2019.01526

Figure Lengend Snippet: Assessment of rate of killing. Synchronous cultures were subjected to 3 x EC 50 concentrations of macrocyclic compounds or dihydroartemisinin (DHA) for 6 or 12 h, followed by washing to remove the inhibitor and incubating in the growth medium in the absence of compounds to monitor recovery. (A) Compounds added at the schizont stage, (B) treatment at the trophozoite stage, and (C) ring stage culture exposed to the compound. Compounds were added at a stage where they exhibit block in cell cycle progression.

Article Snippet: A library of 2,400 macrocyclic compounds was purchased from the commercial vendor Asinex (Winston-Salem, NC) for validation.

Techniques: Blocking Assay