Journal:

Article Title: Expression of β 1 Integrins in Sensory Neurons of the Dorsal Root Ganglion and Their Functions in Neurite Outgrowth on Two Laminin Isoforms

doi:

Figure Lengend Snippet: Differential effects of integrin-α-subunit–specific antibodies on human DRG neurite outgrowth on laminin isoforms AeB1eB2e and AmB1eB2e: fluorescent micrographs of postfixation, Dil-labeled human DRG neurons cultured 16 hr on theAeB1eB2e (A, C, E, G) or the AmB1eB2e (B, D, F, H) isoform of laminin in the absence of antibodies (A, B) or in the presence of mAb anti-β1 (C, D), anti-α1β1 (E, F), or anti-α3β1 (G, H). Antibody concentrations used are given in Materials and Methods. Scale bar, 50 μm.

Article Snippet: All of the monoclonal antibodies (mAbs) were previously characterized as function-blocking antibodies. mAbs PIE6 (anti- α 2 β 1; IgG1), PIB5 (anti- α 3 β 1; IgG1), and PID6 (anti- α 5 β 1; IgG3) have been characterized ( Wayner and Carter, 1987 ) and were purchased as ascites from Telios Inc. (La Jolla, CA). mAbs A2B2 (anti- β 1; IgG1) and S2G3 (anti- α 1 β 1; IgM) have been characterized previously ( Hall et al., 1990 ).

Techniques: Labeling, Cell Culture

Journal:

Article Title: Expression of β 1 Integrins in Sensory Neurons of the Dorsal Root Ganglion and Their Functions in Neurite Outgrowth on Two Laminin Isoforms

doi:

Figure Lengend Snippet: Effect of anti-α1β1 on PC12 cell attachment to laminins AeB1eB2e or AmB1eB2e. a, PC 12 cell attachment to various concentrations of AeB1eB2e laminin in the absence (open circles) or in the presence (solid circles) of anti-α1 (mAb 3A3, 10 μg/ml). b, PC 12 cell attachment to various concentrations of AmB1eB2e in the absence (open circles) or in the presence (solid circles) of anti-α1 (mAb 3A3, 10 μg/ml).

Article Snippet: All of the monoclonal antibodies (mAbs) were previously characterized as function-blocking antibodies. mAbs PIE6 (anti- α 2 β 1; IgG1), PIB5 (anti- α 3 β 1; IgG1), and PID6 (anti- α 5 β 1; IgG3) have been characterized ( Wayner and Carter, 1987 ) and were purchased as ascites from Telios Inc. (La Jolla, CA). mAbs A2B2 (anti- β 1; IgG1) and S2G3 (anti- α 1 β 1; IgM) have been characterized previously ( Hall et al., 1990 ).

Techniques: Cell Attachment Assay