Journal: bioRxiv

Article Title: CD1a-Mediated Presentation of Canonical Microbial Peptides to T Cells

doi: 10.64898/2026.05.05.723095

Figure Lengend Snippet: (A) LCD4.G, LCD4.C, and LCD4.D T cell lines responses to M. tuberculosis (mTB son), M. leprae (mLEP son), M. smegmatis (mSMEG) and non-mycobacterial lysates. (B) LCD4.G and LCD4.CD T cell lines were tested for recognition of known CD1-presented antigens, including dideoxymycobactin (DDM), glucose monomycolate (GMM), mycolic acid, mannose-capped lipoarabinomannan (manLAM), and mannosyl phosphodolichol (MPD). (C) Effect of proteinase K and heat treatment of the M. tuberculosis Triton X-114 extract on T cell proliferation. TX-114 extract was added to the T cell assay after one of four treatments: untreated; proteinase K treatment followed by heat inactivation; heat treatment alone; or incubation with heat-inactivated proteinase K. Data indicate mean ± SEM of triplicate values and are representative of three independent experiments (A–C).

Article Snippet: The M. tuberculosis (H37Rv) Triton X-114 extracted proteins (50 mg) were lyophilized with 7.25 M PlusOne urea (Fisher), 0.4% 3-10 ZOOM carrier ampholytes (ThermoFisher), 1.6% 4-7 pharmalytes, 1% N-octylthioglucoside (EMD Chemicals, Inc) and 2 mM DL-Dithiothreitol (DTT, Fisher).

Techniques: Incubation

Journal: bioRxiv

Article Title: CD1a-Mediated Presentation of Canonical Microbial Peptides to T Cells

doi: 10.64898/2026.05.05.723095

Figure Lengend Snippet: (A) The M. tuberculosis Triton X-114 extract was subjected to preparative IEF, and each fraction was analyzed by Western blot and screened for T cell activation. (B) Identification of candidate proteins by proteomic analysis (LC–MS/MS) of the active fractions. Active fractions stimulating each T cell line were identified by T cell “Western blot” assay. (C) Proliferative response of LCD4.G to purified recombinant lipoproteins derived from the 25-kDa M. tuberculosis lipoglycoprotein LppX. mTB son, M. tuberculosis sonicated. mSMEG, M. smegmatis . Data indicate mean of triplicate values for LCD4.G T cell line and are representative of three independent experiments (A–C). See also Figures S2 and S5.

Article Snippet: The M. tuberculosis (H37Rv) Triton X-114 extracted proteins (50 mg) were lyophilized with 7.25 M PlusOne urea (Fisher), 0.4% 3-10 ZOOM carrier ampholytes (ThermoFisher), 1.6% 4-7 pharmalytes, 1% N-octylthioglucoside (EMD Chemicals, Inc) and 2 mM DL-Dithiothreitol (DTT, Fisher).

Techniques: Western Blot, Activation Assay, Liquid Chromatography with Mass Spectroscopy, Purification, Recombinant, Derivative Assay, Sonication

Journal: medRxiv

Article Title: Molecular epidemiology of rifampicin resistant Mycobacterium tuberculosis in Vietnam

doi: 10.64898/2026.04.20.26351312

Figure Lengend Snippet: Geography of main M. tuberculosis lineages in Vietnam. A) Maximum-liklihood tree of 287 M. tuberculosis isolates from the VQUIN MDR trial. Nodes in bold indicate lineage 2.2.1. Red stars used to indicate isolates from household contacts B) Proportion of lineages represented in each region sampled. Quang Nam was excluded as it only had a single representative isolate (sublineage 2.2.1).

Article Snippet: New genome sequences were phylogenetically contextualised to publicly-available M. tuberculosis genome sequences sampled in Vietnam from National Center for Biotechnology Information (NCBI) Sequence Read Archives (SRA).

Techniques:

Journal: medRxiv

Article Title: Direct detection and quantification of Mycobacterium tuberculosis from clinical samples by high-resolution melt qPCR

doi: 10.64898/2026.03.07.26347851

Figure Lengend Snippet: (A) Amplification plot of H37Rv replicates targeting the RD9 gene. (B) Corresponding melt curve showing a specific melting temperature (Tm) of ∼75°C. H37Rv DNA was serially diluted from 10 6 to 10 1 copies per reaction and analysed in triplicate. (C) Amplification plot obtained after 40 cycles for the dilutions, including the no-template control (NTC). (D) Corresponding melt curve showing a Tm of 73.7 ± 0.12°C. (E) Standard curve generated from the 10-fold serial dilutions, plotting cycle threshold (Ct) versus log copy number per reaction.

Article Snippet: Using the M. tuberculosis H37Rv (ATCC #25618DQ) reference strain, we performed asymmetric PCR with Vent (exo-) DNA polymerase (New England Biolabs).

Techniques: Amplification, Control, Generated