Journal: Frontiers in Microbiology

Article Title: Development and optimization of an easy to interpret loop-mediated isothermal amplification (LAMP) assay for the identification of bacterial pathogens causing childhood pneumonia

doi: 10.3389/fmicb.2026.1748456

Figure Lengend Snippet: Sensitivity of the LAMP reaction for the detection of S. pneumoniae , S. aureus , H. influenzae , and M. pneumoniae. Visual LoD determined for the detection of S. pneumoniae (3.9 ×10 3 CFU/mL), S. aureus (1.7 ×10 5 CFU/mL), H. influenzae (8.2 ×10 3 CFU/mL), and M. pneumoniae (1.27 ×10 3 genome copies/reaction) (A) . Verification of amplification by 2% agarose gel electrophoresis (B) .

Article Snippet: Initially, tests included panel bacteria: S. pneumoniae ATCC 49619, S. aureus ATCC 25923, H. influenzae ATCC 49766, and purified DNA from M. pneumoniae (ATCC 29342DQ).

Techniques: Amplification, Agarose Gel Electrophoresis

Journal: Frontiers in Microbiology

Article Title: Development and optimization of an easy to interpret loop-mediated isothermal amplification (LAMP) assay for the identification of bacterial pathogens causing childhood pneumonia

doi: 10.3389/fmicb.2026.1748456

Figure Lengend Snippet: Sensitivity of the designed primers for the detection of K. pneumoniae. Visual LoD (A) . The calculated LoD was 1.5 ×10 4 CFU/mL. Amplification was confirmed by 2% agarose gel electrophoresis (B) .

Article Snippet: Initially, tests included panel bacteria: S. pneumoniae ATCC 49619, S. aureus ATCC 25923, H. influenzae ATCC 49766, and purified DNA from M. pneumoniae (ATCC 29342DQ).

Techniques: Amplification, Agarose Gel Electrophoresis

Journal: Frontiers in Microbiology

Article Title: Development and optimization of an easy to interpret loop-mediated isothermal amplification (LAMP) assay for the identification of bacterial pathogens causing childhood pneumonia

doi: 10.3389/fmicb.2026.1748456

Figure Lengend Snippet: Relationship between time to positivity and bacterial load for LAMP detection of S. pneumoniae , S. aureus , and H. influenzae . As the bacterial concentration decreased, the time required for detection increased across all three pathogens. For S. pneumoniae , high concentrations produced a clear positive result within 35 min. However, concentrations near the LoD (10 4 and 10 3 CFU/mL) required more than 55 min to be considered positive. Similarly, S. aureus showed a positive signal at 35 min when tested at high concentrations, whereas lower concentrations (around 10 4 CFU/mL) required over 45. In the case of H. influenzae , the highest concentration yielded a visible positive reaction at 25 min, while lower concentrations needed up to 45 min. Transition phase: In all cases, there was a stage where tubes began to show a greenish signal, indicating the onset of positivity, although the color had not yet fully developed to a strong green signal.

Article Snippet: Initially, tests included panel bacteria: S. pneumoniae ATCC 49619, S. aureus ATCC 25923, H. influenzae ATCC 49766, and purified DNA from M. pneumoniae (ATCC 29342DQ).

Techniques: Concentration Assay, Produced

Journal: PLOS One

Article Title: Genomic insights into the expansion of meropenem-resistant GPSC1-CC320 Streptococcus pneumoniae serotype 19A isolates from children under 5 years of age with invasive infections, 2018–2024

doi: 10.1371/journal.pone.0325870

Figure Lengend Snippet: (A) Annual distribution of serotype 19A among 104 S. pneumoniae isolates from children <5 years of age with IPD in South Korea, 2018–2024. (B) Comparison of PCV13 versus non-PCV13 isolates (left) and serotype 19A versus non-19A isolates (right) between the periods 2018–2022 and 2023–2024. Statistical significance was determined using Fisher’s exact test (left: p = 0.0023; right: p = 0.0154).

Article Snippet: After confirming the identification of S. pneumoniae , the isolates were stored in Microbank vials (Pro-lab Diagnostics, Richmond Hill, Canada) at −80°C until further testing.

Techniques: Comparison

Journal: PLOS One

Article Title: Genomic insights into the expansion of meropenem-resistant GPSC1-CC320 Streptococcus pneumoniae serotype 19A isolates from children under 5 years of age with invasive infections, 2018–2024

doi: 10.1371/journal.pone.0325870

Figure Lengend Snippet: Phylogenetic tree of 11 serotype 19A isolates of S. pneumoniae clustered with 1300 isolates of GPSC1.

Article Snippet: After confirming the identification of S. pneumoniae , the isolates were stored in Microbank vials (Pro-lab Diagnostics, Richmond Hill, Canada) at −80°C until further testing.

Techniques:

Journal: NPJ Biofilms and Microbiomes

Article Title: Engineered Mycoplasma pneumoniae targeting dual-species bacterial biofilms: a novel strategy against infections

doi: 10.1038/s41522-025-00835-2

Figure Lengend Snippet: a Graphical representation of the antibiofilm payloads secreted by M. pneumoniae strain CV8_HAD. b Western blot analysis of CV8_HAD lysate and supernatant (SN) showing expression of the payloads; the CV8 chassis and the loaded ribosomal protein RL7 were used as controls. c Representative images of crystal violet assays used to quantify S. aureus (strain Sa15981) or P. aeruginosa (strain PAO1) biofilms after treating with SN from CV8 strains. CV8_D expresses payload D only; CV8_HA expresses payloads H and A. d Plot showing the quantification of biofilm dissolution by CV8 strains SN treatments in vitro, using the crystal violet assay. Data are shown as the mean of three independent experiments ± SEM. *** p < 0.001, ****p < 0.0001 by one-way Anova followed by the post-hoc Tukey’s multiple comparison tests.

Article Snippet: The attenuated strain CV8 derives from the wild-type M. pneumoniae strain M129 (ATCC 29342, subtype 1), in which genes mpn133 and mpn372 were deleted, and the mpn051 gene was replaced by the gpsA gene from Mycoplasma penetrans .

Techniques: Western Blot, Expressing, Dissolution, In Vitro, Crystal Violet Assay, Comparison