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gene exp ly6e hs00158942 m1  (Thermo Fisher)
91
Thermo Fisher gene exp ly6e hs00158942 m1
Identification of a novel IRF2BP2 mutation. (A) Sanger sequencing chromatograms of the IRF2BP2 gene showing a heterozygous de novo missense mutation (c.1663T>A; p. Cys555Ser) in the patient, which is absent in both parents. (B) Heatmap revealing the relative messenger RNA expression levels of IFI44L , <t>LY6E</t> , and MX1 in PBMCs from the patient and a healthy control, as measured using quantitative polymerase chain reaction. Expression levels are normalized to GAPDH and calculated using the comparative CT method. Red indicates higher expression, and blue indicates lower expression relative to the control. FC, fold change.
Gene Exp Ly6e Hs00158942 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene exp ly6e hs00158942 m1/product/Thermo Fisher
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gene exp ly6e hs00158942 m1 - by Bioz Stars, 2026-04
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ly6e  (Proteintech)
93
Proteintech ly6e
<t>LY6E</t> is identified as a key downstream effector of nuclear PD-L1 in metastasis.​​ A ​​ Left: Venn diagram illustrating the strategy for identifying downstream targets of IFN-γ/nuclear PD-L1 from RNA-seq data of PD-L1 WT and KO 4T1 cells treated with or without 50 ng/mL IFN-γ for 24 h. Right: Heatmap of four candidate genes. ​​ B – C ​​ qPCR validation of Cldn1, Ly6e, Anxa8, and Gnb4 mRNA expression in the indicated treatment groups of 4T1 cells ( n = 4). ​​ D ​​ Western blot analysis of Claudin-1 and Ly6E protein expression in PD-L1 WT and KO 4T1 cells treated with or without 50 ng/mL IFN-γ for 24 h. ​​ E – F ​​ qPCR analysis of CLDN1 and LY6E mRNA expression in PD-L1 WT and PD-L1 KO MDA-MB-231 cells treated with or without 50 ng/mL IFN-γ for 48 h. ​​ G – H ​​ Western blot analysis of Claudin-1 and Ly6E protein expression in PD-L1 WT and KO MDA-MB-231 cells treated with or without 50 ng/mL IFN-γ for 48 h. I ​​ Analysis of LY6E and CD274 mRNA expression in TCGA TNBC tumor and adjacent normal tissue samples. ​​ J ​​ Correlation analysis of LY6E and CD274 mRNA expression in TCGA TNBC samples. ​​ K ​​ Representative images of lungs and quantification of metastatic nodules from LY6E overexpression cells. Data are presented as mean ± SD. Student’s t-test was used for two-group data analysis, while One Way ANOVA was used for multiple-group data analysis
Ly6e, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ly6e/product/Proteintech
Average 93 stars, based on 1 article reviews
ly6e - by Bioz Stars, 2026-04
93/100 stars
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rabbit anti ly6e  (Proteintech)
93
Proteintech rabbit anti ly6e
<t>LY6E</t> is identified as a key downstream effector of nuclear PD-L1 in metastasis.​​ A ​​ Left: Venn diagram illustrating the strategy for identifying downstream targets of IFN-γ/nuclear PD-L1 from RNA-seq data of PD-L1 WT and KO 4T1 cells treated with or without 50 ng/mL IFN-γ for 24 h. Right: Heatmap of four candidate genes. ​​ B – C ​​ qPCR validation of Cldn1, Ly6e, Anxa8, and Gnb4 mRNA expression in the indicated treatment groups of 4T1 cells ( n = 4). ​​ D ​​ Western blot analysis of Claudin-1 and Ly6E protein expression in PD-L1 WT and KO 4T1 cells treated with or without 50 ng/mL IFN-γ for 24 h. ​​ E – F ​​ qPCR analysis of CLDN1 and LY6E mRNA expression in PD-L1 WT and PD-L1 KO MDA-MB-231 cells treated with or without 50 ng/mL IFN-γ for 48 h. ​​ G – H ​​ Western blot analysis of Claudin-1 and Ly6E protein expression in PD-L1 WT and KO MDA-MB-231 cells treated with or without 50 ng/mL IFN-γ for 48 h. I ​​ Analysis of LY6E and CD274 mRNA expression in TCGA TNBC tumor and adjacent normal tissue samples. ​​ J ​​ Correlation analysis of LY6E and CD274 mRNA expression in TCGA TNBC samples. ​​ K ​​ Representative images of lungs and quantification of metastatic nodules from LY6E overexpression cells. Data are presented as mean ± SD. Student’s t-test was used for two-group data analysis, while One Way ANOVA was used for multiple-group data analysis
Rabbit Anti Ly6e, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti ly6e/product/Proteintech
Average 93 stars, based on 1 article reviews
rabbit anti ly6e - by Bioz Stars, 2026-04
93/100 stars
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dual targeting tce against ly6e and b7−h4  (Genentech inc)
90
Genentech inc dual targeting tce against ly6e and b7−h4
<t>LY6E</t> is identified as a key downstream effector of nuclear PD-L1 in metastasis.​​ A ​​ Left: Venn diagram illustrating the strategy for identifying downstream targets of IFN-γ/nuclear PD-L1 from RNA-seq data of PD-L1 WT and KO 4T1 cells treated with or without 50 ng/mL IFN-γ for 24 h. Right: Heatmap of four candidate genes. ​​ B – C ​​ qPCR validation of Cldn1, Ly6e, Anxa8, and Gnb4 mRNA expression in the indicated treatment groups of 4T1 cells ( n = 4). ​​ D ​​ Western blot analysis of Claudin-1 and Ly6E protein expression in PD-L1 WT and KO 4T1 cells treated with or without 50 ng/mL IFN-γ for 24 h. ​​ E – F ​​ qPCR analysis of CLDN1 and LY6E mRNA expression in PD-L1 WT and PD-L1 KO MDA-MB-231 cells treated with or without 50 ng/mL IFN-γ for 48 h. ​​ G – H ​​ Western blot analysis of Claudin-1 and Ly6E protein expression in PD-L1 WT and KO MDA-MB-231 cells treated with or without 50 ng/mL IFN-γ for 48 h. I ​​ Analysis of LY6E and CD274 mRNA expression in TCGA TNBC tumor and adjacent normal tissue samples. ​​ J ​​ Correlation analysis of LY6E and CD274 mRNA expression in TCGA TNBC samples. ​​ K ​​ Representative images of lungs and quantification of metastatic nodules from LY6E overexpression cells. Data are presented as mean ± SD. Student’s t-test was used for two-group data analysis, while One Way ANOVA was used for multiple-group data analysis
Dual Targeting Tce Against Ly6e And B7−H4, supplied by Genentech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dual targeting tce against ly6e and b7−h4/product/Genentech inc
Average 90 stars, based on 1 article reviews
dual targeting tce against ly6e and b7−h4 - by Bioz Stars, 2026-04
90/100 stars
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anti ly6e  (Novus Biologicals)
92
Novus Biologicals anti ly6e
<t>LY6E</t> is identified as a key downstream effector of nuclear PD-L1 in metastasis.​​ A ​​ Left: Venn diagram illustrating the strategy for identifying downstream targets of IFN-γ/nuclear PD-L1 from RNA-seq data of PD-L1 WT and KO 4T1 cells treated with or without 50 ng/mL IFN-γ for 24 h. Right: Heatmap of four candidate genes. ​​ B – C ​​ qPCR validation of Cldn1, Ly6e, Anxa8, and Gnb4 mRNA expression in the indicated treatment groups of 4T1 cells ( n = 4). ​​ D ​​ Western blot analysis of Claudin-1 and Ly6E protein expression in PD-L1 WT and KO 4T1 cells treated with or without 50 ng/mL IFN-γ for 24 h. ​​ E – F ​​ qPCR analysis of CLDN1 and LY6E mRNA expression in PD-L1 WT and PD-L1 KO MDA-MB-231 cells treated with or without 50 ng/mL IFN-γ for 48 h. ​​ G – H ​​ Western blot analysis of Claudin-1 and Ly6E protein expression in PD-L1 WT and KO MDA-MB-231 cells treated with or without 50 ng/mL IFN-γ for 48 h. I ​​ Analysis of LY6E and CD274 mRNA expression in TCGA TNBC tumor and adjacent normal tissue samples. ​​ J ​​ Correlation analysis of LY6E and CD274 mRNA expression in TCGA TNBC samples. ​​ K ​​ Representative images of lungs and quantification of metastatic nodules from LY6E overexpression cells. Data are presented as mean ± SD. Student’s t-test was used for two-group data analysis, while One Way ANOVA was used for multiple-group data analysis
Anti Ly6e, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti ly6e/product/Novus Biologicals
Average 92 stars, based on 1 article reviews
anti ly6e - by Bioz Stars, 2026-04
92/100 stars
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ly6e rabbit polyclonal ab  (Danaher Inc)
86
Danaher Inc ly6e rabbit polyclonal ab
<t>LY6E</t> expression and relationship with clinical characteristics in PC. ( A ) LY6E expression between PC tissues and normal tissues across TCGA + GTEx, GSE16515, GSE32676, GSE15471, and GSE55463 datasets. ( B ) LY6E expression difference in PC with different clinical subgroups. ( C ) Clinical characteristic difference in LY6E low-expression (C1) and high-expression (C2) subgroups. ( D ) Correlation between LY6E expression, age, clinical stage, tumor grade, and survival status. *, p < 0.05.
Ly6e Rabbit Polyclonal Ab, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ly6e rabbit polyclonal ab/product/Danaher Inc
Average 86 stars, based on 1 article reviews
ly6e rabbit polyclonal ab - by Bioz Stars, 2026-04
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Image Search Results


Identification of a novel IRF2BP2 mutation. (A) Sanger sequencing chromatograms of the IRF2BP2 gene showing a heterozygous de novo missense mutation (c.1663T>A; p. Cys555Ser) in the patient, which is absent in both parents. (B) Heatmap revealing the relative messenger RNA expression levels of IFI44L , LY6E , and MX1 in PBMCs from the patient and a healthy control, as measured using quantitative polymerase chain reaction. Expression levels are normalized to GAPDH and calculated using the comparative CT method. Red indicates higher expression, and blue indicates lower expression relative to the control. FC, fold change.

Journal: Frontiers in Immunology

Article Title: Case Report: Novel IRF2BP2 variant in a Japanese patient with impaired B-cell differentiation, Th1 polarization, and systemic immune dysregulation

doi: 10.3389/fimmu.2025.1662899

Figure Lengend Snippet: Identification of a novel IRF2BP2 mutation. (A) Sanger sequencing chromatograms of the IRF2BP2 gene showing a heterozygous de novo missense mutation (c.1663T>A; p. Cys555Ser) in the patient, which is absent in both parents. (B) Heatmap revealing the relative messenger RNA expression levels of IFI44L , LY6E , and MX1 in PBMCs from the patient and a healthy control, as measured using quantitative polymerase chain reaction. Expression levels are normalized to GAPDH and calculated using the comparative CT method. Red indicates higher expression, and blue indicates lower expression relative to the control. FC, fold change.

Article Snippet: All primers and probes were obtained from Applied Biosystems ( IFI44L [Hs00915292_m1], LY6E [Hs00158942_m1], MX1 [Hs00895608_m1], and GAPDH [Hs02758991_g1]).

Techniques: Mutagenesis, Sequencing, RNA Expression, Control, Real-time Polymerase Chain Reaction, Expressing

LY6E is identified as a key downstream effector of nuclear PD-L1 in metastasis.​​ A ​​ Left: Venn diagram illustrating the strategy for identifying downstream targets of IFN-γ/nuclear PD-L1 from RNA-seq data of PD-L1 WT and KO 4T1 cells treated with or without 50 ng/mL IFN-γ for 24 h. Right: Heatmap of four candidate genes. ​​ B – C ​​ qPCR validation of Cldn1, Ly6e, Anxa8, and Gnb4 mRNA expression in the indicated treatment groups of 4T1 cells ( n = 4). ​​ D ​​ Western blot analysis of Claudin-1 and Ly6E protein expression in PD-L1 WT and KO 4T1 cells treated with or without 50 ng/mL IFN-γ for 24 h. ​​ E – F ​​ qPCR analysis of CLDN1 and LY6E mRNA expression in PD-L1 WT and PD-L1 KO MDA-MB-231 cells treated with or without 50 ng/mL IFN-γ for 48 h. ​​ G – H ​​ Western blot analysis of Claudin-1 and Ly6E protein expression in PD-L1 WT and KO MDA-MB-231 cells treated with or without 50 ng/mL IFN-γ for 48 h. I ​​ Analysis of LY6E and CD274 mRNA expression in TCGA TNBC tumor and adjacent normal tissue samples. ​​ J ​​ Correlation analysis of LY6E and CD274 mRNA expression in TCGA TNBC samples. ​​ K ​​ Representative images of lungs and quantification of metastatic nodules from LY6E overexpression cells. Data are presented as mean ± SD. Student’s t-test was used for two-group data analysis, while One Way ANOVA was used for multiple-group data analysis

Journal: Breast Cancer Research : BCR

Article Title: Nuclear PD-L1 drives IFN-γ-promoted lung metastasis of triple-negative breast cancer via POLR2A-mediated transcriptional activation of LY6E

doi: 10.1186/s13058-025-02193-5

Figure Lengend Snippet: LY6E is identified as a key downstream effector of nuclear PD-L1 in metastasis.​​ A ​​ Left: Venn diagram illustrating the strategy for identifying downstream targets of IFN-γ/nuclear PD-L1 from RNA-seq data of PD-L1 WT and KO 4T1 cells treated with or without 50 ng/mL IFN-γ for 24 h. Right: Heatmap of four candidate genes. ​​ B – C ​​ qPCR validation of Cldn1, Ly6e, Anxa8, and Gnb4 mRNA expression in the indicated treatment groups of 4T1 cells ( n = 4). ​​ D ​​ Western blot analysis of Claudin-1 and Ly6E protein expression in PD-L1 WT and KO 4T1 cells treated with or without 50 ng/mL IFN-γ for 24 h. ​​ E – F ​​ qPCR analysis of CLDN1 and LY6E mRNA expression in PD-L1 WT and PD-L1 KO MDA-MB-231 cells treated with or without 50 ng/mL IFN-γ for 48 h. ​​ G – H ​​ Western blot analysis of Claudin-1 and Ly6E protein expression in PD-L1 WT and KO MDA-MB-231 cells treated with or without 50 ng/mL IFN-γ for 48 h. I ​​ Analysis of LY6E and CD274 mRNA expression in TCGA TNBC tumor and adjacent normal tissue samples. ​​ J ​​ Correlation analysis of LY6E and CD274 mRNA expression in TCGA TNBC samples. ​​ K ​​ Representative images of lungs and quantification of metastatic nodules from LY6E overexpression cells. Data are presented as mean ± SD. Student’s t-test was used for two-group data analysis, while One Way ANOVA was used for multiple-group data analysis

Article Snippet: Proteins (20–30 μg) were separated by SDS-PAGE, transferred to nitrocellulose (NC) membranes, and probed with primary antibodies against PD-L1 (CST, #13684; #60475), POLR2A (CST, #2629), LY6E (Proteintech, 22144-1-AP), Claudin-1 (Proteintech, 13050-1-AP), STAT1 and phosphorylated STAT1 (CST, #14994; #9167), and GAPDH (Proteintech, 60004-1-Ig).

Techniques: RNA Sequencing, Biomarker Discovery, Expressing, Western Blot, Over Expression

Nuclear PD-L1 forms a transcriptional complex with POLR2A to upregulate LY6E.​​ A ​​ Browser view of published ChIP-seq data showing lack of PD-L1 enrichment at the LY6E promoter region (highlighted in yellow) in MDA-MB-231 cells. ​​ B ​​ Transcription factors (TFs) predicted to bind the LY6E promoter in MDA-MB-231 cells, ranked by transcriptional potential score. ​​ C ​​ Venn diagram identifying POLR2A as the only TF overlapping between factors predicted to bind LY6E ( B ) and factors found to interact with PD-L1 by Co-IP/MS in MDA-MB-231 cells. ​​ D ​​ Browser view of ChIP-seq data showing POLR2A enrichment at the LY6E promoter region (highlighted in yellow) in MDA-MB-231 cells. ​​ E ​​ Predicted structural model of the PD-L1/POLR2A interaction generated by ZDOCK. Key interacting residues are labeled: Salt bridge (PD-L1 R140 - POLR2A E517), Electrostatic interactions (PD-L1 K136/K185 - POLR2A D452/D663). ​​ F ​​ Co-immunoprecipitation assay in MDA-MB-231 cells to detect protein interaction between POLR2A and PD-L1

Journal: Breast Cancer Research : BCR

Article Title: Nuclear PD-L1 drives IFN-γ-promoted lung metastasis of triple-negative breast cancer via POLR2A-mediated transcriptional activation of LY6E

doi: 10.1186/s13058-025-02193-5

Figure Lengend Snippet: Nuclear PD-L1 forms a transcriptional complex with POLR2A to upregulate LY6E.​​ A ​​ Browser view of published ChIP-seq data showing lack of PD-L1 enrichment at the LY6E promoter region (highlighted in yellow) in MDA-MB-231 cells. ​​ B ​​ Transcription factors (TFs) predicted to bind the LY6E promoter in MDA-MB-231 cells, ranked by transcriptional potential score. ​​ C ​​ Venn diagram identifying POLR2A as the only TF overlapping between factors predicted to bind LY6E ( B ) and factors found to interact with PD-L1 by Co-IP/MS in MDA-MB-231 cells. ​​ D ​​ Browser view of ChIP-seq data showing POLR2A enrichment at the LY6E promoter region (highlighted in yellow) in MDA-MB-231 cells. ​​ E ​​ Predicted structural model of the PD-L1/POLR2A interaction generated by ZDOCK. Key interacting residues are labeled: Salt bridge (PD-L1 R140 - POLR2A E517), Electrostatic interactions (PD-L1 K136/K185 - POLR2A D452/D663). ​​ F ​​ Co-immunoprecipitation assay in MDA-MB-231 cells to detect protein interaction between POLR2A and PD-L1

Article Snippet: Proteins (20–30 μg) were separated by SDS-PAGE, transferred to nitrocellulose (NC) membranes, and probed with primary antibodies against PD-L1 (CST, #13684; #60475), POLR2A (CST, #2629), LY6E (Proteintech, 22144-1-AP), Claudin-1 (Proteintech, 13050-1-AP), STAT1 and phosphorylated STAT1 (CST, #14994; #9167), and GAPDH (Proteintech, 60004-1-Ig).

Techniques: ChIP-sequencing, Co-Immunoprecipitation Assay, Generated, Labeling

LY6E expression and relationship with clinical characteristics in PC. ( A ) LY6E expression between PC tissues and normal tissues across TCGA + GTEx, GSE16515, GSE32676, GSE15471, and GSE55463 datasets. ( B ) LY6E expression difference in PC with different clinical subgroups. ( C ) Clinical characteristic difference in LY6E low-expression (C1) and high-expression (C2) subgroups. ( D ) Correlation between LY6E expression, age, clinical stage, tumor grade, and survival status. *, p < 0.05.

Journal: Scientific Reports

Article Title: Lymphocyte antigen 6 family member E suppresses apoptosis and promotes pancreatic cancer growth and migration via Wnt/β-catenin pathway activation

doi: 10.1038/s41598-024-70764-1

Figure Lengend Snippet: LY6E expression and relationship with clinical characteristics in PC. ( A ) LY6E expression between PC tissues and normal tissues across TCGA + GTEx, GSE16515, GSE32676, GSE15471, and GSE55463 datasets. ( B ) LY6E expression difference in PC with different clinical subgroups. ( C ) Clinical characteristic difference in LY6E low-expression (C1) and high-expression (C2) subgroups. ( D ) Correlation between LY6E expression, age, clinical stage, tumor grade, and survival status. *, p < 0.05.

Article Snippet: The following primary Abs were used: LY6E rabbit polyclonal Ab (ab201098, 1:1000, Abcam), β-catenin rabbit monoclonal Ab (ab32572, 1:1000, Abcam), GSK3β rabbit monoclonal Ab (ab32391, 1:1000, Abcam), p-GSK3β rabbit monoclonal Ab (ab75814, 1:1000, Abcam), E-cadherin rabbit monoclonal Ab (ab40772, 1:1000, Abcam), N-cadherin rabbit monoclonal Ab (ab76011, 1:1000, Abcam), Vimentin rabbit monoclonal Ab (ab92547, 1:1000, Abcam), Bcl-2 rabbit monoclonal Ab (ab32124, 1:1000, Abcam), Bax rabbit monoclonal Ab (ab32503, 1:1000, Abcam), Cleaved caspase3 rabbit monoclonal Ab (ab32042, 1:1000, Abcam) and GAPDH mouse monoclonal Ab (ab8245, 1:1000, Abcam).

Techniques: Expressing

Correlation of prognostic ability of LY6E expression. ( A ) Distribution, survival status plot, survival time of LY6E expression in TCGA-PAAD project. Red dots and blue dots represent patients with high and low expression of LY6E, respectively. ( B ) Survival analysis of LY6E low-expression and high-expression PC in TCGA-PAAD project. ( C ) ROC curves analyses between LY6E low-expression and high-expression PC in TCGA-PAAD project. ( D ) Univariate and multivariate Cox regression analyses in TCGA-PAAD project. ( E ) A nomogram of LY6E expression and age for PC. ( F ) A calibration plot for assessing the predictive ability of the nomogram at 1- and 3-year.

Journal: Scientific Reports

Article Title: Lymphocyte antigen 6 family member E suppresses apoptosis and promotes pancreatic cancer growth and migration via Wnt/β-catenin pathway activation

doi: 10.1038/s41598-024-70764-1

Figure Lengend Snippet: Correlation of prognostic ability of LY6E expression. ( A ) Distribution, survival status plot, survival time of LY6E expression in TCGA-PAAD project. Red dots and blue dots represent patients with high and low expression of LY6E, respectively. ( B ) Survival analysis of LY6E low-expression and high-expression PC in TCGA-PAAD project. ( C ) ROC curves analyses between LY6E low-expression and high-expression PC in TCGA-PAAD project. ( D ) Univariate and multivariate Cox regression analyses in TCGA-PAAD project. ( E ) A nomogram of LY6E expression and age for PC. ( F ) A calibration plot for assessing the predictive ability of the nomogram at 1- and 3-year.

Article Snippet: The following primary Abs were used: LY6E rabbit polyclonal Ab (ab201098, 1:1000, Abcam), β-catenin rabbit monoclonal Ab (ab32572, 1:1000, Abcam), GSK3β rabbit monoclonal Ab (ab32391, 1:1000, Abcam), p-GSK3β rabbit monoclonal Ab (ab75814, 1:1000, Abcam), E-cadherin rabbit monoclonal Ab (ab40772, 1:1000, Abcam), N-cadherin rabbit monoclonal Ab (ab76011, 1:1000, Abcam), Vimentin rabbit monoclonal Ab (ab92547, 1:1000, Abcam), Bcl-2 rabbit monoclonal Ab (ab32124, 1:1000, Abcam), Bax rabbit monoclonal Ab (ab32503, 1:1000, Abcam), Cleaved caspase3 rabbit monoclonal Ab (ab32042, 1:1000, Abcam) and GAPDH mouse monoclonal Ab (ab8245, 1:1000, Abcam).

Techniques: Expressing

Relationship of LY6E and immunity. ( A ) Correlation coefficient value between LY6E expression and immune cell infiltration. ( B ) Immune cell infiltration difference between LY6E low-expression and high-expression PC. ( C , D , E , F , G , H , I , J , and K ) Relationship between LY6E expression and memory B cells, activated dendritic cells, macrophage M0, activated NK cells, plasma cells, CD8 + T cells, activated memory CD4 + T cells, naive B cells, and tumor mutation burden. ( L ) Correlation between LY6E and other immune-related genes. Red indicates positive correlation, while blue stands for negative correlation. The darker the color, the stronger the correlation coefficient. *, p < 0.05. **, p < 0.01. ***, p < 0.001.

Journal: Scientific Reports

Article Title: Lymphocyte antigen 6 family member E suppresses apoptosis and promotes pancreatic cancer growth and migration via Wnt/β-catenin pathway activation

doi: 10.1038/s41598-024-70764-1

Figure Lengend Snippet: Relationship of LY6E and immunity. ( A ) Correlation coefficient value between LY6E expression and immune cell infiltration. ( B ) Immune cell infiltration difference between LY6E low-expression and high-expression PC. ( C , D , E , F , G , H , I , J , and K ) Relationship between LY6E expression and memory B cells, activated dendritic cells, macrophage M0, activated NK cells, plasma cells, CD8 + T cells, activated memory CD4 + T cells, naive B cells, and tumor mutation burden. ( L ) Correlation between LY6E and other immune-related genes. Red indicates positive correlation, while blue stands for negative correlation. The darker the color, the stronger the correlation coefficient. *, p < 0.05. **, p < 0.01. ***, p < 0.001.

Article Snippet: The following primary Abs were used: LY6E rabbit polyclonal Ab (ab201098, 1:1000, Abcam), β-catenin rabbit monoclonal Ab (ab32572, 1:1000, Abcam), GSK3β rabbit monoclonal Ab (ab32391, 1:1000, Abcam), p-GSK3β rabbit monoclonal Ab (ab75814, 1:1000, Abcam), E-cadherin rabbit monoclonal Ab (ab40772, 1:1000, Abcam), N-cadherin rabbit monoclonal Ab (ab76011, 1:1000, Abcam), Vimentin rabbit monoclonal Ab (ab92547, 1:1000, Abcam), Bcl-2 rabbit monoclonal Ab (ab32124, 1:1000, Abcam), Bax rabbit monoclonal Ab (ab32503, 1:1000, Abcam), Cleaved caspase3 rabbit monoclonal Ab (ab32042, 1:1000, Abcam) and GAPDH mouse monoclonal Ab (ab8245, 1:1000, Abcam).

Techniques: Expressing, Mutagenesis

LY6E is highly expressed in PC and knockdown of LY6E impairs PC cell growth in vitro. ( A ) IHC staining of LY6E expression in the tumor and normal tissues of PC patients. Scale bar = 100 μm. ( B ) Western blotting analysis of LY6E protein expression in tumor tissues and normal tissues of PC patients. GAPDH was used as a control. ( C ) Western blotting analysis of LY6E protein expression in a human pancreatic cell line (HPNE) and PC cell lines (PANC-1, BxPC-3, and Patu8988). GAPDH was used as the control. ( D ) qRT-PCR analysis of LY6E mRNA transcription in BxPC-3 and Patu8988 cells transfected with lenti-shLY6E-1 and lenti-shLY6E-2. GAPDH was used as the control. ( E ) Western blotting analysis of LY6E in BxPC-3 and Patu8988 cells transfected with lenti-shLY6E-1 and lenti-shLY6E-2. GAPDH was used as the control. ( F ) Cell viability at 0, 24, 48 and 72 h were detected after cell was seeded using the CCK-8 assay. OD450 nm absorbance was measured. *** represents the lenti-shLY6E-1 treatment group compared to NC, with p < 0.001. ### represents the lenti-shLY6E-2 treatment group compared to NC, with p < 0.001. ( G ) Colony formation analysis of BxPC-3 and Patu8988 cells that were transfected with lenti-shLY6E-1 and lenti-shLY6E-2 (14 d after cell seeding). The number of colonies in each well was counted. ( H ) IFC staining of Ki67 in BxPC-3 and Patu8988 cells transfected with lenti-shLY6E-1 and lenti-shLY6E-2.*, p < 0.05.

Journal: Scientific Reports

Article Title: Lymphocyte antigen 6 family member E suppresses apoptosis and promotes pancreatic cancer growth and migration via Wnt/β-catenin pathway activation

doi: 10.1038/s41598-024-70764-1

Figure Lengend Snippet: LY6E is highly expressed in PC and knockdown of LY6E impairs PC cell growth in vitro. ( A ) IHC staining of LY6E expression in the tumor and normal tissues of PC patients. Scale bar = 100 μm. ( B ) Western blotting analysis of LY6E protein expression in tumor tissues and normal tissues of PC patients. GAPDH was used as a control. ( C ) Western blotting analysis of LY6E protein expression in a human pancreatic cell line (HPNE) and PC cell lines (PANC-1, BxPC-3, and Patu8988). GAPDH was used as the control. ( D ) qRT-PCR analysis of LY6E mRNA transcription in BxPC-3 and Patu8988 cells transfected with lenti-shLY6E-1 and lenti-shLY6E-2. GAPDH was used as the control. ( E ) Western blotting analysis of LY6E in BxPC-3 and Patu8988 cells transfected with lenti-shLY6E-1 and lenti-shLY6E-2. GAPDH was used as the control. ( F ) Cell viability at 0, 24, 48 and 72 h were detected after cell was seeded using the CCK-8 assay. OD450 nm absorbance was measured. *** represents the lenti-shLY6E-1 treatment group compared to NC, with p < 0.001. ### represents the lenti-shLY6E-2 treatment group compared to NC, with p < 0.001. ( G ) Colony formation analysis of BxPC-3 and Patu8988 cells that were transfected with lenti-shLY6E-1 and lenti-shLY6E-2 (14 d after cell seeding). The number of colonies in each well was counted. ( H ) IFC staining of Ki67 in BxPC-3 and Patu8988 cells transfected with lenti-shLY6E-1 and lenti-shLY6E-2.*, p < 0.05.

Article Snippet: The following primary Abs were used: LY6E rabbit polyclonal Ab (ab201098, 1:1000, Abcam), β-catenin rabbit monoclonal Ab (ab32572, 1:1000, Abcam), GSK3β rabbit monoclonal Ab (ab32391, 1:1000, Abcam), p-GSK3β rabbit monoclonal Ab (ab75814, 1:1000, Abcam), E-cadherin rabbit monoclonal Ab (ab40772, 1:1000, Abcam), N-cadherin rabbit monoclonal Ab (ab76011, 1:1000, Abcam), Vimentin rabbit monoclonal Ab (ab92547, 1:1000, Abcam), Bcl-2 rabbit monoclonal Ab (ab32124, 1:1000, Abcam), Bax rabbit monoclonal Ab (ab32503, 1:1000, Abcam), Cleaved caspase3 rabbit monoclonal Ab (ab32042, 1:1000, Abcam) and GAPDH mouse monoclonal Ab (ab8245, 1:1000, Abcam).

Techniques: Knockdown, In Vitro, Immunohistochemistry, Expressing, Western Blot, Control, Quantitative RT-PCR, Transfection, CCK-8 Assay, Staining

Knockdown of LY6E promotes apoptosis by regulating cleaved caspase 3, Bcl-2, and Bax protein expression in PC. ( A ) Cell apoptosis was detected in BxPC-3 and Patu8988 cells that were transfected with lenti-shLY6E-1 and lentishLY6E-2. Flow cytometry was used to determine the percentage of cells that were apoptotic. ( B ) Western blotting analysis of cleaved caspase 3, Bcl-2, and Bax protein expression in BxPC-3 and Patu8988 cells that were transfected with lenti-shLY6E-1 and lentishLY6E-2. GAPDH was used as the control. ( C ) IFC staining of cleaved caspase 3 in BxPC-3 and Patu8988 cells transfected with lenti-shLY6E-1 and lenti-shLY6E-2. *, p < 0.05.

Journal: Scientific Reports

Article Title: Lymphocyte antigen 6 family member E suppresses apoptosis and promotes pancreatic cancer growth and migration via Wnt/β-catenin pathway activation

doi: 10.1038/s41598-024-70764-1

Figure Lengend Snippet: Knockdown of LY6E promotes apoptosis by regulating cleaved caspase 3, Bcl-2, and Bax protein expression in PC. ( A ) Cell apoptosis was detected in BxPC-3 and Patu8988 cells that were transfected with lenti-shLY6E-1 and lentishLY6E-2. Flow cytometry was used to determine the percentage of cells that were apoptotic. ( B ) Western blotting analysis of cleaved caspase 3, Bcl-2, and Bax protein expression in BxPC-3 and Patu8988 cells that were transfected with lenti-shLY6E-1 and lentishLY6E-2. GAPDH was used as the control. ( C ) IFC staining of cleaved caspase 3 in BxPC-3 and Patu8988 cells transfected with lenti-shLY6E-1 and lenti-shLY6E-2. *, p < 0.05.

Article Snippet: The following primary Abs were used: LY6E rabbit polyclonal Ab (ab201098, 1:1000, Abcam), β-catenin rabbit monoclonal Ab (ab32572, 1:1000, Abcam), GSK3β rabbit monoclonal Ab (ab32391, 1:1000, Abcam), p-GSK3β rabbit monoclonal Ab (ab75814, 1:1000, Abcam), E-cadherin rabbit monoclonal Ab (ab40772, 1:1000, Abcam), N-cadherin rabbit monoclonal Ab (ab76011, 1:1000, Abcam), Vimentin rabbit monoclonal Ab (ab92547, 1:1000, Abcam), Bcl-2 rabbit monoclonal Ab (ab32124, 1:1000, Abcam), Bax rabbit monoclonal Ab (ab32503, 1:1000, Abcam), Cleaved caspase3 rabbit monoclonal Ab (ab32042, 1:1000, Abcam) and GAPDH mouse monoclonal Ab (ab8245, 1:1000, Abcam).

Techniques: Knockdown, Expressing, Transfection, Flow Cytometry, Western Blot, Control, Staining

Knockdown of LY6E suppresses migration and invasion of PC by regulating vimentin, E-Cadherin, and N-Cadherin protein expression. ( A ) Wound healing assay of BxPC-3 and Patu8988 cells that were transfected with lenti-shLY6E-1 and lentishLY6E-2. ( B ) Transwell assay of BxPC-3 and Patu8988 cells that were transfected with lenti-shLY6E-1 and lentishLY6E-2. ( C ) Western blotting analysis of certain migration-related proteins including vimentin, E-Cadherin, and N-Cadherin in BxPC-3 and Patu8988 cells that were transfected with lenti-shLY6E-1 and lentishLY6E-2. GAPDH was used as the control. ( D ) IFC staining of vimentin in BxPC-3 and Patu8988 cells transfected with lenti-shLY6E-1 and lenti-shLY6E-2. *, p < 0.05.

Journal: Scientific Reports

Article Title: Lymphocyte antigen 6 family member E suppresses apoptosis and promotes pancreatic cancer growth and migration via Wnt/β-catenin pathway activation

doi: 10.1038/s41598-024-70764-1

Figure Lengend Snippet: Knockdown of LY6E suppresses migration and invasion of PC by regulating vimentin, E-Cadherin, and N-Cadherin protein expression. ( A ) Wound healing assay of BxPC-3 and Patu8988 cells that were transfected with lenti-shLY6E-1 and lentishLY6E-2. ( B ) Transwell assay of BxPC-3 and Patu8988 cells that were transfected with lenti-shLY6E-1 and lentishLY6E-2. ( C ) Western blotting analysis of certain migration-related proteins including vimentin, E-Cadherin, and N-Cadherin in BxPC-3 and Patu8988 cells that were transfected with lenti-shLY6E-1 and lentishLY6E-2. GAPDH was used as the control. ( D ) IFC staining of vimentin in BxPC-3 and Patu8988 cells transfected with lenti-shLY6E-1 and lenti-shLY6E-2. *, p < 0.05.

Article Snippet: The following primary Abs were used: LY6E rabbit polyclonal Ab (ab201098, 1:1000, Abcam), β-catenin rabbit monoclonal Ab (ab32572, 1:1000, Abcam), GSK3β rabbit monoclonal Ab (ab32391, 1:1000, Abcam), p-GSK3β rabbit monoclonal Ab (ab75814, 1:1000, Abcam), E-cadherin rabbit monoclonal Ab (ab40772, 1:1000, Abcam), N-cadherin rabbit monoclonal Ab (ab76011, 1:1000, Abcam), Vimentin rabbit monoclonal Ab (ab92547, 1:1000, Abcam), Bcl-2 rabbit monoclonal Ab (ab32124, 1:1000, Abcam), Bax rabbit monoclonal Ab (ab32503, 1:1000, Abcam), Cleaved caspase3 rabbit monoclonal Ab (ab32042, 1:1000, Abcam) and GAPDH mouse monoclonal Ab (ab8245, 1:1000, Abcam).

Techniques: Knockdown, Migration, Expressing, Wound Healing Assay, Transfection, Transwell Assay, Western Blot, Control, Staining

Overexpression of LY6E suppresses apoptosis, enhances proliferation and migration of PC cells. ( A ) CCK8 assay indicated that overexpression of LY6E augmented PC proliferation. ( B ) Clone formation assay indicated more colonies in LY6E overexpression in PC than in control. (C) The effect of LY6E overexpression on PC apoptosis was analyzed by flow cytometry. ( D ) Transwell showed that LY6E increased the number of migrating PC. *, p < 0.05. **, p < 0.01. ***, p < 0.001.

Journal: Scientific Reports

Article Title: Lymphocyte antigen 6 family member E suppresses apoptosis and promotes pancreatic cancer growth and migration via Wnt/β-catenin pathway activation

doi: 10.1038/s41598-024-70764-1

Figure Lengend Snippet: Overexpression of LY6E suppresses apoptosis, enhances proliferation and migration of PC cells. ( A ) CCK8 assay indicated that overexpression of LY6E augmented PC proliferation. ( B ) Clone formation assay indicated more colonies in LY6E overexpression in PC than in control. (C) The effect of LY6E overexpression on PC apoptosis was analyzed by flow cytometry. ( D ) Transwell showed that LY6E increased the number of migrating PC. *, p < 0.05. **, p < 0.01. ***, p < 0.001.

Article Snippet: The following primary Abs were used: LY6E rabbit polyclonal Ab (ab201098, 1:1000, Abcam), β-catenin rabbit monoclonal Ab (ab32572, 1:1000, Abcam), GSK3β rabbit monoclonal Ab (ab32391, 1:1000, Abcam), p-GSK3β rabbit monoclonal Ab (ab75814, 1:1000, Abcam), E-cadherin rabbit monoclonal Ab (ab40772, 1:1000, Abcam), N-cadherin rabbit monoclonal Ab (ab76011, 1:1000, Abcam), Vimentin rabbit monoclonal Ab (ab92547, 1:1000, Abcam), Bcl-2 rabbit monoclonal Ab (ab32124, 1:1000, Abcam), Bax rabbit monoclonal Ab (ab32503, 1:1000, Abcam), Cleaved caspase3 rabbit monoclonal Ab (ab32042, 1:1000, Abcam) and GAPDH mouse monoclonal Ab (ab8245, 1:1000, Abcam).

Techniques: Over Expression, Migration, CCK-8 Assay, Tube Formation Assay, Control, Flow Cytometry

Knockdown of LY6E down-regulated the Wnt/beta-catenin signaling pathway. ( A ) Gene expression profile of LY6E low-expression and high-expression PC in TCGA-PAAD. G1 and G2 represent groups with high and low expression of LY6E mRNA. Columns and rows denote patients and genes. ( B ) Up-regulated and down-regulated genes in LY6E low-expression and high-expression PC in TCGA-PAAD. Red dots and blue dots indicate upregulated and downregulated genes. The x-axis represents fold change, while the y-axis reflects statistical significance. ( C ) GO/KEGG analysis of up-regulated and down-regulated genes in LY6E low-expression and high-expression PC in TCGA-PAAD. ( D ) Correlation analysis of LY6E expression and CTNNB1 expression. Points represent patients, with the horizontal and vertical axes displaying the expression levels of LY6E and CTNNB1, respectively. ( E ) Western blotting analysis of β-catenin, GSK3β, and p-GSK3β in BxPC-3 and Patu8988 cells that were transfected with lenti-shLY6E-1 and lentishLY6E-2. GAPDH was used as the control. ( F ) IFC staining of β-catenin in BxPC-3 and Patu8988 cells transfected with lenti-shLY6E-1 and lenti-shLY6E-2.

Journal: Scientific Reports

Article Title: Lymphocyte antigen 6 family member E suppresses apoptosis and promotes pancreatic cancer growth and migration via Wnt/β-catenin pathway activation

doi: 10.1038/s41598-024-70764-1

Figure Lengend Snippet: Knockdown of LY6E down-regulated the Wnt/beta-catenin signaling pathway. ( A ) Gene expression profile of LY6E low-expression and high-expression PC in TCGA-PAAD. G1 and G2 represent groups with high and low expression of LY6E mRNA. Columns and rows denote patients and genes. ( B ) Up-regulated and down-regulated genes in LY6E low-expression and high-expression PC in TCGA-PAAD. Red dots and blue dots indicate upregulated and downregulated genes. The x-axis represents fold change, while the y-axis reflects statistical significance. ( C ) GO/KEGG analysis of up-regulated and down-regulated genes in LY6E low-expression and high-expression PC in TCGA-PAAD. ( D ) Correlation analysis of LY6E expression and CTNNB1 expression. Points represent patients, with the horizontal and vertical axes displaying the expression levels of LY6E and CTNNB1, respectively. ( E ) Western blotting analysis of β-catenin, GSK3β, and p-GSK3β in BxPC-3 and Patu8988 cells that were transfected with lenti-shLY6E-1 and lentishLY6E-2. GAPDH was used as the control. ( F ) IFC staining of β-catenin in BxPC-3 and Patu8988 cells transfected with lenti-shLY6E-1 and lenti-shLY6E-2.

Article Snippet: The following primary Abs were used: LY6E rabbit polyclonal Ab (ab201098, 1:1000, Abcam), β-catenin rabbit monoclonal Ab (ab32572, 1:1000, Abcam), GSK3β rabbit monoclonal Ab (ab32391, 1:1000, Abcam), p-GSK3β rabbit monoclonal Ab (ab75814, 1:1000, Abcam), E-cadherin rabbit monoclonal Ab (ab40772, 1:1000, Abcam), N-cadherin rabbit monoclonal Ab (ab76011, 1:1000, Abcam), Vimentin rabbit monoclonal Ab (ab92547, 1:1000, Abcam), Bcl-2 rabbit monoclonal Ab (ab32124, 1:1000, Abcam), Bax rabbit monoclonal Ab (ab32503, 1:1000, Abcam), Cleaved caspase3 rabbit monoclonal Ab (ab32042, 1:1000, Abcam) and GAPDH mouse monoclonal Ab (ab8245, 1:1000, Abcam).

Techniques: Knockdown, Expressing, Western Blot, Transfection, Control, Staining

Knockdown of LY6E inhibits pancreatic tumor growth in vivo. ( A , B , and C ) Tumor image, end-stage tumor weight, and tumor volume ( D ) Western blotting analysis of Vimentin, E-Cadherin, and N-Cadherin protein expression in tumor tissues. GAPDH was used as the control. ( E ) HE staining and IHC staining of Ki67, β-catenin, and Cleaved caspase 3 in tumor tissues. *, p < 0.05.

Journal: Scientific Reports

Article Title: Lymphocyte antigen 6 family member E suppresses apoptosis and promotes pancreatic cancer growth and migration via Wnt/β-catenin pathway activation

doi: 10.1038/s41598-024-70764-1

Figure Lengend Snippet: Knockdown of LY6E inhibits pancreatic tumor growth in vivo. ( A , B , and C ) Tumor image, end-stage tumor weight, and tumor volume ( D ) Western blotting analysis of Vimentin, E-Cadherin, and N-Cadherin protein expression in tumor tissues. GAPDH was used as the control. ( E ) HE staining and IHC staining of Ki67, β-catenin, and Cleaved caspase 3 in tumor tissues. *, p < 0.05.

Article Snippet: The following primary Abs were used: LY6E rabbit polyclonal Ab (ab201098, 1:1000, Abcam), β-catenin rabbit monoclonal Ab (ab32572, 1:1000, Abcam), GSK3β rabbit monoclonal Ab (ab32391, 1:1000, Abcam), p-GSK3β rabbit monoclonal Ab (ab75814, 1:1000, Abcam), E-cadherin rabbit monoclonal Ab (ab40772, 1:1000, Abcam), N-cadherin rabbit monoclonal Ab (ab76011, 1:1000, Abcam), Vimentin rabbit monoclonal Ab (ab92547, 1:1000, Abcam), Bcl-2 rabbit monoclonal Ab (ab32124, 1:1000, Abcam), Bax rabbit monoclonal Ab (ab32503, 1:1000, Abcam), Cleaved caspase3 rabbit monoclonal Ab (ab32042, 1:1000, Abcam) and GAPDH mouse monoclonal Ab (ab8245, 1:1000, Abcam).

Techniques: Knockdown, In Vivo, Western Blot, Expressing, Control, Staining, Immunohistochemistry

The rescue experiment verifies that LY6E promotes PC progression through Wnt/beta-catenin signaling pathway. ( A ) Transwell assay confirmed the restoration of invasion of BxPC-3 and Patu8988 cells transfected with lenti-shLY6E after being treated by LiCl. ( B and C ) Western blotting analysis of Vimentin, E-Cadherin, N-Cadherin, β-catenin, GSK3β, and p-GSK3β protein expression in BxPC-3 and Patu8988 cells transfected with lenti-shLY6E after treated by LiCl. GAPDH was used as the control. *, p < 0.05.

Journal: Scientific Reports

Article Title: Lymphocyte antigen 6 family member E suppresses apoptosis and promotes pancreatic cancer growth and migration via Wnt/β-catenin pathway activation

doi: 10.1038/s41598-024-70764-1

Figure Lengend Snippet: The rescue experiment verifies that LY6E promotes PC progression through Wnt/beta-catenin signaling pathway. ( A ) Transwell assay confirmed the restoration of invasion of BxPC-3 and Patu8988 cells transfected with lenti-shLY6E after being treated by LiCl. ( B and C ) Western blotting analysis of Vimentin, E-Cadherin, N-Cadherin, β-catenin, GSK3β, and p-GSK3β protein expression in BxPC-3 and Patu8988 cells transfected with lenti-shLY6E after treated by LiCl. GAPDH was used as the control. *, p < 0.05.

Article Snippet: The following primary Abs were used: LY6E rabbit polyclonal Ab (ab201098, 1:1000, Abcam), β-catenin rabbit monoclonal Ab (ab32572, 1:1000, Abcam), GSK3β rabbit monoclonal Ab (ab32391, 1:1000, Abcam), p-GSK3β rabbit monoclonal Ab (ab75814, 1:1000, Abcam), E-cadherin rabbit monoclonal Ab (ab40772, 1:1000, Abcam), N-cadherin rabbit monoclonal Ab (ab76011, 1:1000, Abcam), Vimentin rabbit monoclonal Ab (ab92547, 1:1000, Abcam), Bcl-2 rabbit monoclonal Ab (ab32124, 1:1000, Abcam), Bax rabbit monoclonal Ab (ab32503, 1:1000, Abcam), Cleaved caspase3 rabbit monoclonal Ab (ab32042, 1:1000, Abcam) and GAPDH mouse monoclonal Ab (ab8245, 1:1000, Abcam).

Techniques: Transwell Assay, Transfection, Western Blot, Expressing, Control