Journal: International Journal of Pharmaceutics: X

Article Title: Multifunctional armored nanoemulsion of elemene combining ferroptosis induction and gut homeostasis restoration in colorectal cancer therapy

doi: 10.1016/j.ijpx.2026.100517

Figure Lengend Snippet: In vivo antitumor therapy of LMP@EL-CNE. (A) Schematic illustration of the process of establishing, treating, and monitoring the orthotopic colorectal model. (B) Representative IVIS bioluminescence images of mice bearing orthotopic colorectal tumors over time in PBS, LMP@CNE, LMP@EL-CNE, and EL-OE groups. (C) Corresponding individual quantitative analysis of relative bioluminescence intensity on the 7th, 14th, and 21st days after different treatments. (D) Survival, and (E) Changes in body weight of mice in various groups. (F) Representative Ki67 and H&E staining of mouse tumor sections after various treatments, with scale bars of 100 μm. Data are shown as mean ± SEM ( n = 6). Statistical significance was determined by one-way ANOVA followed by Tukey's post hoc test; ns p > 0.05, * p < 0.05, *** p < 0.001.

Article Snippet: Whole-body fluorescence images were acquired using an IVIS Lumina LT imaging system (PerkinElmer, USA).

Techniques: In Vivo, Staining

Journal: Bioactive Materials

Article Title: M2 macrophage-derived exosomes delivering haptoglobin and interleukin-10 plasmids for synergistic therapy of intracerebral hemorrhage

doi: 10.1016/j.bioactmat.2026.01.047

Figure Lengend Snippet: Validation of M2-exo targeting, Hp/IL-10 transfection expression, and Hp/Hb binding. (a) Fluorescence imaging showing cellular uptake of ICG and M2-exo@ICG by M1 microglia. (b,c) Flow cytometry and corresponding quantification of RhB and M2-exo@RhB internalized by M1 microglia (n = 3). (d,e) Schematic illustration and quantitative analysis of the in vitro phagocytosis-release kinetics of M2-exo@RhB in BV2 under ICH-mimicking stimulation (n = 6). (f) Fluorescence images showing Hp and IL-10 expression in M1 microglia treated with M2-exo@HI for 12, 24, 48, 72 h. (g) Mean fluorescence intensity (MFI) quantification of Hp and IL-10 expression (n = 3). (h,i) ELISA measurements of secreted Hp and IL-10 protein levels (n = 3). (j,k) qPCR analysis of relative Hp and IL-10 mRNA expression (n = 3). (l) Western blot detection of Hp and IL-10 protein expression. (m) Densitometric quantification of Hp and IL-10 protein levels from Western blot (n = 3). (n) Co-immunoprecipitation assay confirming the formation of Hp-Hb complex. Data are presented as mean ± SD. Statistical significance was calculated by unpaired Student's t -test (c and e), and one-way ANOVA with Tukey's multiple comparisons test (g-k and m).

Article Snippet: The libraries were constructed using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, CA, USA).

Techniques: Biomarker Discovery, Transfection, Expressing, Binding Assay, Fluorescence, Imaging, Flow Cytometry, In Vitro, Enzyme-linked Immunosorbent Assay, Western Blot, Co-Immunoprecipitation Assay