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(A) . Transwell analysis the migrated cells after TGF-β1 (5ng/mL), 74DHF (100nM), and VC (500μM) treatment. Scale bar: 100μm. The quantification of the stained cells was shown on the right (n = 3). (B) . Real time PCR of EMT marker Acta2 and Cdh1 expression in BEAS-2B cells treated by TGF-β1 (5ng/mL), 74DHF (100nM) or VC (500μM) (n = 3). (C) WB analysis of α-SMA, CDH1, COL1A1 and <t>AKT1</t> in BEAS-2B cells treated by TGF-β1 (5ng/mL), 74DHF (100nM) or VC (500μM) for 24 hours (n = 3). The grey intensity was quantified on the right. Data are presented as the means ± SEM compared by one-way ANOVA with Bonferroni's Multiple Comparison Test. (*, P < 0.05, **, P < 0.01, ***, P < 0.001).
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(A) . Transwell analysis the migrated cells after TGF-β1 (5ng/mL), 74DHF (100nM), and VC (500μM) treatment. Scale bar: 100μm. The quantification of the stained cells was shown on the right (n = 3). (B) . Real time PCR of EMT marker Acta2 and Cdh1 expression in BEAS-2B cells treated by TGF-β1 (5ng/mL), 74DHF (100nM) or VC (500μM) (n = 3). (C) WB analysis of α-SMA, CDH1, COL1A1 and <t>AKT1</t> in BEAS-2B cells treated by TGF-β1 (5ng/mL), 74DHF (100nM) or VC (500μM) for 24 hours (n = 3). The grey intensity was quantified on the right. Data are presented as the means ± SEM compared by one-way ANOVA with Bonferroni's Multiple Comparison Test. (*, P < 0.05, **, P < 0.01, ***, P < 0.001).
Col1a1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) . Transwell analysis the migrated cells after TGF-β1 (5ng/mL), 74DHF (100nM), and VC (500μM) treatment. Scale bar: 100μm. The quantification of the stained cells was shown on the right (n = 3). (B) . Real time PCR of EMT marker Acta2 and Cdh1 expression in BEAS-2B cells treated by TGF-β1 (5ng/mL), 74DHF (100nM) or VC (500μM) (n = 3). (C) WB analysis of α-SMA, CDH1, COL1A1 and <t>AKT1</t> in BEAS-2B cells treated by TGF-β1 (5ng/mL), 74DHF (100nM) or VC (500μM) for 24 hours (n = 3). The grey intensity was quantified on the right. Data are presented as the means ± SEM compared by one-way ANOVA with Bonferroni's Multiple Comparison Test. (*, P < 0.05, **, P < 0.01, ***, P < 0.001).
Codon, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) . Transwell analysis the migrated cells after TGF-β1 (5ng/mL), 74DHF (100nM), and VC (500μM) treatment. Scale bar: 100μm. The quantification of the stained cells was shown on the right (n = 3). (B) . Real time PCR of EMT marker Acta2 and Cdh1 expression in BEAS-2B cells treated by TGF-β1 (5ng/mL), 74DHF (100nM) or VC (500μM) (n = 3). (C) WB analysis of α-SMA, CDH1, COL1A1 and <t>AKT1</t> in BEAS-2B cells treated by TGF-β1 (5ng/mL), 74DHF (100nM) or VC (500μM) for 24 hours (n = 3). The grey intensity was quantified on the right. Data are presented as the means ± SEM compared by one-way ANOVA with Bonferroni's Multiple Comparison Test. (*, P < 0.05, **, P < 0.01, ***, P < 0.001).
Anti Lrp6 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) . Transwell analysis the migrated cells after TGF-β1 (5ng/mL), 74DHF (100nM), and VC (500μM) treatment. Scale bar: 100μm. The quantification of the stained cells was shown on the right (n = 3). (B) . Real time PCR of EMT marker Acta2 and Cdh1 expression in BEAS-2B cells treated by TGF-β1 (5ng/mL), 74DHF (100nM) or VC (500μM) (n = 3). (C) WB analysis of α-SMA, CDH1, COL1A1 and <t>AKT1</t> in BEAS-2B cells treated by TGF-β1 (5ng/mL), 74DHF (100nM) or VC (500μM) for 24 hours (n = 3). The grey intensity was quantified on the right. Data are presented as the means ± SEM compared by one-way ANOVA with Bonferroni's Multiple Comparison Test. (*, P < 0.05, **, P < 0.01, ***, P < 0.001).
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(A) . Transwell analysis the migrated cells after TGF-β1 (5ng/mL), 74DHF (100nM), and VC (500μM) treatment. Scale bar: 100μm. The quantification of the stained cells was shown on the right (n = 3). (B) . Real time PCR of EMT marker Acta2 and Cdh1 expression in BEAS-2B cells treated by TGF-β1 (5ng/mL), 74DHF (100nM) or VC (500μM) (n = 3). (C) WB analysis of α-SMA, CDH1, COL1A1 and <t>AKT1</t> in BEAS-2B cells treated by TGF-β1 (5ng/mL), 74DHF (100nM) or VC (500μM) for 24 hours (n = 3). The grey intensity was quantified on the right. Data are presented as the means ± SEM compared by one-way ANOVA with Bonferroni's Multiple Comparison Test. (*, P < 0.05, **, P < 0.01, ***, P < 0.001).
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lrp6  (Santa Cruz Biotechnology)
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a Split violin plots showing the expression distribution of WNT and NOTCH pathway components in DE (red) and DPL (blue). Each dot represents a single cell. Adjacent pie charts quantify the percentage of cells within the DE population expressing specific WNT and NOTCH ligands. b Dot plots illustrating the dynamic expression of WNT and NOTCH pathway genes in DE (left panel, red color scale) and DPL (right panel, blue color scale) across developmental stages. Dot size is proportional to the percentage of cells expressing the gene, and color intensity reflects the average z-score normalized expression level. PCW, post-conception weeks; Y, years. c Line graphs tracking the average expression of key WNT ( WNT6 , WNT7B , WNT10B ) and NOTCH ( JAG1 ) ligands within the DE population over time. d IF validation of the spatial localization of key WNT and NOTCH pathway proteins across different developmental stages. Target proteins (WNT6, WNT7B, WNT10B, DKK1, SFRP1, <t>LRP6,</t> LEF1, JAG1, NOTCH2, HEY1) are shown in green or red, with nuclei counterstained with DAPI (blue). Dotted lines outline the epithelial-mesenchymal boundary. Insets show magnified views of the indicated regions. Images are representative of experiments that were independently repeated three times with similar results ( n = 3 biological replicates). PT permanent tooth. Scale bars: 100 μm. Data in ( a – c ) are derived from scRNA-seq analysis of n = 2 biological replicates per developmental stage.
Lrp6, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) . Transwell analysis the migrated cells after TGF-β1 (5ng/mL), 74DHF (100nM), and VC (500μM) treatment. Scale bar: 100μm. The quantification of the stained cells was shown on the right (n = 3). (B) . Real time PCR of EMT marker Acta2 and Cdh1 expression in BEAS-2B cells treated by TGF-β1 (5ng/mL), 74DHF (100nM) or VC (500μM) (n = 3). (C) WB analysis of α-SMA, CDH1, COL1A1 and AKT1 in BEAS-2B cells treated by TGF-β1 (5ng/mL), 74DHF (100nM) or VC (500μM) for 24 hours (n = 3). The grey intensity was quantified on the right. Data are presented as the means ± SEM compared by one-way ANOVA with Bonferroni's Multiple Comparison Test. (*, P < 0.05, **, P < 0.01, ***, P < 0.001).

Journal: PLOS One

Article Title: Deciphering the synergistic mechanism of a novel flavonoid-antioxidant combination for asthma by combining systems pharmacology and experimental validation

doi: 10.1371/journal.pone.0346165

Figure Lengend Snippet: (A) . Transwell analysis the migrated cells after TGF-β1 (5ng/mL), 74DHF (100nM), and VC (500μM) treatment. Scale bar: 100μm. The quantification of the stained cells was shown on the right (n = 3). (B) . Real time PCR of EMT marker Acta2 and Cdh1 expression in BEAS-2B cells treated by TGF-β1 (5ng/mL), 74DHF (100nM) or VC (500μM) (n = 3). (C) WB analysis of α-SMA, CDH1, COL1A1 and AKT1 in BEAS-2B cells treated by TGF-β1 (5ng/mL), 74DHF (100nM) or VC (500μM) for 24 hours (n = 3). The grey intensity was quantified on the right. Data are presented as the means ± SEM compared by one-way ANOVA with Bonferroni's Multiple Comparison Test. (*, P < 0.05, **, P < 0.01, ***, P < 0.001).

Article Snippet: The membrane was incubated with primary antibody against α-SMA (Abcam, ab7817), CDH1 (CST, 1065), COL1A1 (CST, 3395), AKT1 (CST, 2938), and GAPDH (Abcam, ab70699) over night.

Techniques: Staining, Real-time Polymerase Chain Reaction, Marker, Expressing, Comparison

a Split violin plots showing the expression distribution of WNT and NOTCH pathway components in DE (red) and DPL (blue). Each dot represents a single cell. Adjacent pie charts quantify the percentage of cells within the DE population expressing specific WNT and NOTCH ligands. b Dot plots illustrating the dynamic expression of WNT and NOTCH pathway genes in DE (left panel, red color scale) and DPL (right panel, blue color scale) across developmental stages. Dot size is proportional to the percentage of cells expressing the gene, and color intensity reflects the average z-score normalized expression level. PCW, post-conception weeks; Y, years. c Line graphs tracking the average expression of key WNT ( WNT6 , WNT7B , WNT10B ) and NOTCH ( JAG1 ) ligands within the DE population over time. d IF validation of the spatial localization of key WNT and NOTCH pathway proteins across different developmental stages. Target proteins (WNT6, WNT7B, WNT10B, DKK1, SFRP1, LRP6, LEF1, JAG1, NOTCH2, HEY1) are shown in green or red, with nuclei counterstained with DAPI (blue). Dotted lines outline the epithelial-mesenchymal boundary. Insets show magnified views of the indicated regions. Images are representative of experiments that were independently repeated three times with similar results ( n = 3 biological replicates). PT permanent tooth. Scale bars: 100 μm. Data in ( a – c ) are derived from scRNA-seq analysis of n = 2 biological replicates per developmental stage.

Journal: Nature Communications

Article Title: Spatiotemporal interplay between epithelial and mesenchymal cells drives human dentinogenesis

doi: 10.1038/s41467-026-69545-3

Figure Lengend Snippet: a Split violin plots showing the expression distribution of WNT and NOTCH pathway components in DE (red) and DPL (blue). Each dot represents a single cell. Adjacent pie charts quantify the percentage of cells within the DE population expressing specific WNT and NOTCH ligands. b Dot plots illustrating the dynamic expression of WNT and NOTCH pathway genes in DE (left panel, red color scale) and DPL (right panel, blue color scale) across developmental stages. Dot size is proportional to the percentage of cells expressing the gene, and color intensity reflects the average z-score normalized expression level. PCW, post-conception weeks; Y, years. c Line graphs tracking the average expression of key WNT ( WNT6 , WNT7B , WNT10B ) and NOTCH ( JAG1 ) ligands within the DE population over time. d IF validation of the spatial localization of key WNT and NOTCH pathway proteins across different developmental stages. Target proteins (WNT6, WNT7B, WNT10B, DKK1, SFRP1, LRP6, LEF1, JAG1, NOTCH2, HEY1) are shown in green or red, with nuclei counterstained with DAPI (blue). Dotted lines outline the epithelial-mesenchymal boundary. Insets show magnified views of the indicated regions. Images are representative of experiments that were independently repeated three times with similar results ( n = 3 biological replicates). PT permanent tooth. Scale bars: 100 μm. Data in ( a – c ) are derived from scRNA-seq analysis of n = 2 biological replicates per developmental stage.

Article Snippet: Primary antibodies were applied against: DSPP (Abcam, ab216892), Ki67 (Cell Signaling Technology, 9129, Clone D3B5), KRT14 (Abcam, ab119695, Clone LL002), WNT6 (Abcam, ab50030), WNT7B (Proteintech, 10605-1-AP), WNT10B (Abcam, ab70816), LRP6 (Santa Cruz, sc-25317, Clone C-10), JAG1 (Abcam, ab300561, Clone EPR26388-51), NOTCH2 (Abcam, ab313453, Clone EPR28701-38), DKK1 (Abcam, ab61034, Clone EPR4759), SFRP1 (Proteintech, 26460-1-AP), HEY1 (Proteintech, 19929-1-AP), LEF1 (Abcam, ab137872, Clone EPR2029Y), BMP2 (Abcam,ab214821), BMP4 (Abcam, ab39973), GFP(CST, 2956, Clone D5.1) and rabbit IgG isotype control (CST, 3900, Clone DA1E) were added.

Techniques: Expressing, Single Cell, Biomarker Discovery, Derivative Assay