Journal: MedComm

Article Title: Ipriflavone From Aquilaria malaccensis Lam. Exosome‐Like Nanoparticles Targets Prolyl Hydroxylase Domain Protein 2 (PHD2) to Enhance Hypoxia‐Inducible Factor‐α (HIF‐α) Hydroxylation Thereby Alleviating Hypoxia‐Induced Gastrointestinal Mucosal Ferroptosis

doi: 10.1002/mco2.70722

Figure Lengend Snippet: Oral administration of AELNs ameliorated gastric and small intestinal mucosal ferroptosis caused by hypoxia in mice. The mice were housed in a vented hypoxic chamber for 3 days ( n = 10 mice in each group). They received PBS or AELNs (1 × 10 5 particles) via oral gavage on Day 1 and were euthanized on Day 3. Representative photographs showing the microstructures of the murine gastric (A) and small intestinal mucosal cells (B) under transmission electron microscopy (TEM). The red arrows indicate shrunken mitochondria (scale bar: overview, 1 µm; inset, 500 nm). Quantification of Fe 2+ , reactive oxygen species (ROS), lipid peroxidation (LPO), and 4‐hydroxynonenal (4‐HNE) of the gastric (C) and small intestinal (D) mucosa ( n = 10). Western blot analysis of the indicated protein levels in the gastric (E) and small intestinal (F) mucosa assessed by Western blot. (G) Cytokines in the gastric (upper) and small intestinal (lower) mucosa ( n = 10). Error bars indicate mean ± SEM. One‐way ANOVA: * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001; ns, not significant.

Article Snippet: ROS, 4‐HNE, and LPO levels were measured using kits from Elabscience (ROS, E‐BC‐K138‐F; 4‐HNE, E‐EL‐0128c; LPO, E‐BC‐K176‐M; Wuhan, Hubei, China).

Techniques: Transmission Assay, Electron Microscopy, Western Blot

Journal: eBioMedicine

Article Title: Multi-omics identifies oxidative stress, prothrombotic pathways, and lactoperoxidase variants as key factors in COVID-19 severity

doi: 10.1016/j.ebiom.2025.106111

Figure Lengend Snippet: Genetic and expression evidence of the LPO lactoperoxidase gene in COVID-19 severity. (A) Lollipop diagram of LPO showing the 6 coding variants identified in our COVID-19 patient cohort (green = missense, black = truncating). The diagram was obtained using the MutationMapper tool on the cBioPortal for Cancer Genomics ( https://www.cbioportal.org/mutation_mapper ). (B) Normalized LPO mRNA expression levels in patients admitted to ICU and those hospitalised, as retrieved from RNA-seq data on patients' PBMCs. Each dot represents an individual sample; boxplots show the median, interquartile range, and distribution. (C) Meta-analysis of the association between the common rs77996252 SNP in LPO and COVID-19 severity in the RGC database. The Forest plot visualises the individual study ORs, 95% CIs, and the pooled OR. The x-axis is displayed on a log10 scale. Each study's OR, 95% CI, and p-value were displayed alongside the plot. (D) Meta-analysis of the association between rare variants in LPO and COVID-19 severity. The Forest plot visualises the individual study ORs, 95% CIs, and the pooled OR. The x-axis is displayed on a log10 scale. Each study's OR, 95% CI, and p-value were displayed alongside the plot. Data include European cohorts (UK Biobank and Geisinger Health System) from the RGC database and our own Italian cohort. (E) Differential LPO expression in disease vs healthy individuals by protein extension assay. Data were retrieved from the Human Protein Atlas. Each dot represents a disease. The x-axis indicates the p-value of significance whereas the y-axis reports the log2(fold-change) in the expression of LPO protein in diseased patients vs healthy controls.

Article Snippet: Finally, to better understand whether and how LPO levels are altered in COVID-19 or other disease conditions, we explored publicly available data on LPO protein levels in blood from the Human Protein Atlas ( https://www.proteinatlas.org/ENSG00000167419-LPO/blood ).

Techniques: Expressing, Mutagenesis, RNA Sequencing

Journal: International Journal of Chronic Obstructive Pulmonary Disease

Article Title: Myostatin/HIF2α-Mediated Ferroptosis is Involved in Skeletal Muscle Dysfunction in Chronic Obstructive Pulmonary Disease

doi: 10.2147/COPD.S377226

Figure Lengend Snippet: Chronic CS exposure caused ferroptosis in skeletal muscles from mice. ( A ) Cell death was enriched in skeletal muscles from COPD patients and healthy control by GSEA. ( B and C ) Gene expression of Gpx4 and Ncoa4 in skeletal muscles from mice by RNA-sequencing; ( D ) RT-qPCR examined the expression of Gpx4, Slc7a11, Tfr1 , and Ncoa4 in muscles; ( E ) WB showed the protein expression of GPX4 in muscles; ( F ) IF showed the expression of GPX4 in muscles from mice exposed to air and CS; The scale in each image is 50 μm; ( G ) the content of GSH in muscles; ( H ) the level of LPO in muscles determined by colorimetry; ( I ) IF showed the expression of 4-HNE in muscles from mice exposed to air and CS; The scale in each image is 50 μm. n = 5, * P < 0.05, ** P < 0.01, *** P < 0.001, ****P < 0.0001.

Article Snippet: The lipid peroxide (LPO) level in lysates of skeletal muscles and C2C12 myotubes was detected using a lipid peroxidation assay kit (E-BC-K176, Elabscience, Wuhan, China) according to the manufacturer’s protocol.

Techniques: Muscles, Control, Gene Expression, RNA Sequencing, Quantitative RT-PCR, Expressing, Colorimetric Assay

Journal: International Journal of Chronic Obstructive Pulmonary Disease

Article Title: Myostatin/HIF2α-Mediated Ferroptosis is Involved in Skeletal Muscle Dysfunction in Chronic Obstructive Pulmonary Disease

doi: 10.2147/COPD.S377226

Figure Lengend Snippet: CSE stimulation caused ferroptosis in C2C12 myotubes. ( A ) Cellular morphology of C2C12 myoblasts and C2C12 myotubes. The scale in each image is 100 μm; ( B ) the proportion of cell death was detected by flow cytometry with 7AAD staining in Ctrl and 3% CSE-stimulated myotubes; ( C ) RT-qPCR examined the expression of Gpx4, Slc7a11, Slc3a2, Tfr1, Ncoa4, Acls4 and Lpcat3 in C2C12 myotubes; ( D ) WB showed the protein expression of GPX4, MuRF1, Atrogin1, and MSTN in C2C12 myotubes; ( E ) the level of Fe 2+ was detected by flow cytometry with the fluorescent probe; ( F ) the content of GSH in C2C12 myotubes; ( G ) lipid ROS was detected by flow cytometry with C11 BODIPY; ( H ) the level of LPO in C2C12 myotubes. ( I ) The proportion of cell death was detected by flow cytometry with 7AAD staining. UAMC-3203, a ferroptosis inhibitor. *** P < 0.001, **** P < 0.0001.

Article Snippet: The lipid peroxide (LPO) level in lysates of skeletal muscles and C2C12 myotubes was detected using a lipid peroxidation assay kit (E-BC-K176, Elabscience, Wuhan, China) according to the manufacturer’s protocol.

Techniques: Flow Cytometry, Staining, Quantitative RT-PCR, Expressing

Journal: International Journal of Chronic Obstructive Pulmonary Disease

Article Title: Myostatin/HIF2α-Mediated Ferroptosis is Involved in Skeletal Muscle Dysfunction in Chronic Obstructive Pulmonary Disease

doi: 10.2147/COPD.S377226

Figure Lengend Snippet: CSE induced ferroptosis by up-regulating the expression of MSTN. ( A ) WB showed the expression of GPX4 in myotubes; ( B ) the expression of lipid ROS in myotubes by confocal microscopy with C11 BODIPY; The scale in each image is 100 μm; ( C ) the proportion of cell death was detected by flow cytometry with 7AAD staining in different concentration of MSTN treated myotubes; ( D ) WB showed the protein expression of GPX4 and HIF2α; ( E ) histogram of the content of GSH in myotubes; ( F and G ) flow cytometry detected the levels of Fe 2+ and lipid ROS in different concentration of MSTN treated myotubes; ( H ) histogram of the content of LPO in myotubes. * P < 0.05,** P < 0.01,**** P < 0.0001.

Article Snippet: The lipid peroxide (LPO) level in lysates of skeletal muscles and C2C12 myotubes was detected using a lipid peroxidation assay kit (E-BC-K176, Elabscience, Wuhan, China) according to the manufacturer’s protocol.

Techniques: Expressing, Confocal Microscopy, Flow Cytometry, Staining, Concentration Assay

Journal: International Journal of Chronic Obstructive Pulmonary Disease

Article Title: Myostatin/HIF2α-Mediated Ferroptosis is Involved in Skeletal Muscle Dysfunction in Chronic Obstructive Pulmonary Disease

doi: 10.2147/COPD.S377226

Figure Lengend Snippet: Ferroptosis inhibitor intervention alleviated ferroptosis-related indicators caused by MSTN. ( A ) Cell death was detected by flow cytometry; ( B ) the protein expression of GPX4; ( C ) Histogram of the content of GSH; ( D and E ) flow cytometry detected the levels of Fe 2+ and lipid ROS; ( F ) histogram of the content of LPO; ( G ) the expression of lipid ROS in myotubes using confocal microscopy; The scale in each image is 100 μm. * P < 0.05,** P < 0.01,*** P < 0.001, ****P < 0.0001.

Article Snippet: The lipid peroxide (LPO) level in lysates of skeletal muscles and C2C12 myotubes was detected using a lipid peroxidation assay kit (E-BC-K176, Elabscience, Wuhan, China) according to the manufacturer’s protocol.

Techniques: Flow Cytometry, Expressing, Confocal Microscopy

Journal: International Journal of Chronic Obstructive Pulmonary Disease

Article Title: Myostatin/HIF2α-Mediated Ferroptosis is Involved in Skeletal Muscle Dysfunction in Chronic Obstructive Pulmonary Disease

doi: 10.2147/COPD.S377226

Figure Lengend Snippet: HIF2α inhibition alleviated ferroptosis-related indicators caused by CSE. ( A ) Corplot showed the correlation between Hif1α, Epas1, Hif3α , and Gpx4 . ( B ) Gene expression of Hif1α, Epas1 , and Hif3α in skeletal muscles from mice by RNA-sequencing; ( C ) WB showed the protein expression of HIF2α in muscles (up) and C2C12 myotubes (down); ( D ) the proportion of cell death was detected by flow cytometry with 7AAD staining in myotubes; ( E ) RT-qPCR detected the expression of Gpx4 in myotubes; ( F ) the expression of HIF2α and GPX4 were detected by WB; ( G and H ) the levels of Fe 2+ and lipid ROS were detected by flow cytometry; ( I and J ) histogram of the content of LPO and GSH in C2C12 myotubes. HIF2α IN, an inhibitor of HIF2α. * P < 0.05,** P < 0.01,**** P < 0.0001.

Article Snippet: The lipid peroxide (LPO) level in lysates of skeletal muscles and C2C12 myotubes was detected using a lipid peroxidation assay kit (E-BC-K176, Elabscience, Wuhan, China) according to the manufacturer’s protocol.

Techniques: Inhibition, Gene Expression, Muscles, RNA Sequencing, Expressing, Flow Cytometry, Staining, Quantitative RT-PCR

Journal: International Journal of Chronic Obstructive Pulmonary Disease

Article Title: Myostatin/HIF2α-Mediated Ferroptosis is Involved in Skeletal Muscle Dysfunction in Chronic Obstructive Pulmonary Disease

doi: 10.2147/COPD.S377226

Figure Lengend Snippet: HIF2α knocking down alleviated ferroptosis-related indicators caused by CSE. ( A ) WB showed the protein expression of GPX4 and HIF2α; ( B ) the proportion of cell death was detected by flow cytometry with 7AAD staining in myotubes; ( C ) histogram of the content of GSH in myotubes; ( D and E ) flow cytometry detected the levels of Fe 2+ and lipid ROS in myotubes; ( F ) histogram of the content of LPO in myotubes. * P < 0.05,** P < 0.01,*** P < 0.001.

Article Snippet: The lipid peroxide (LPO) level in lysates of skeletal muscles and C2C12 myotubes was detected using a lipid peroxidation assay kit (E-BC-K176, Elabscience, Wuhan, China) according to the manufacturer’s protocol.

Techniques: Expressing, Flow Cytometry, Staining