Journal: Pharmaceutics

Article Title: Tumor Activated Cell Penetrating Peptides to Selectively Deliver Immune Modulatory Drugs

doi: 10.3390/pharmaceutics13030365

Figure Lengend Snippet: ACPPs are cleaved in situ within murine tumors. ( A ) Structural representation of ratiometric activatable cell penetrating peptide probe with intervening MMP-2/9 protease sensitive amino acid sequence. ( B ) Mice with subcutaneous syngeneic LL2 tumors tail vein injected with 10 nanomoles ratiometric ACPP. In situ mouse imaging for Cy5:Cy7 emission ratio shown. Cy5:Cy7 emission ratio pseudocolor scale bar shown on far right. ( C ) Confocal imaging of tissue from LL2 tumor bearing mice injected with ratiometric ACPP. Tissue section containing tumor and adjacent muscle imaged for Cy5 (Red). Nuclei DAPI stained (Blue).

Article Snippet: Murine B16F10 melanoma and LL2 Lewis lung carcinoma cell lines were purchased from ATCC.

Techniques: In Situ, Sequencing, Injection, Imaging, Staining

Journal: Cell reports

Article Title: Analysis of Single-Cell RNA-Seq Identifies Cell-Cell Communication Associated with Tumor Characteristics

doi: 10.1016/j.celrep.2018.10.047

Figure Lengend Snippet: (A) Tumor volume (y axis) of treatment-naive mice measured over time (x axis) . Dashed lines indicate the mean of a syngeneic tumor model, and shaded areas represent 1 SEM (n = 8 for Sa1N, 7 for LL2, 10 for CT26, 9 for EMT6, and 9 for MC38). Instances with no shading result from only one mouse surviving at the measured time points. Linear curves were fit to the log-normalized growth curves, and the slope of fit curves was used as a metric for tumor growth. (B) Quantified growth rates for each model. Each point represents a single mouse, and the horizontal black line indicates the median growth rate used for correlation with interaction scores.(C) Heatmap showing the Spearman correlation of interaction scores (shown in ) with tumor growth. Interactions marked with black circles indicate correlations with p < 0.01. Grey boxes indicate interactions for which the interaction score was zero across all models and no correlation could be computed.(D) Distribution of receptor only, ligand only, and interaction score correlations. Each point represents an interaction (only autocrine interactions between tumor cells are displayed). The x axis represents the correlation of ligand expression alone with tumor growth rate, whereas the y axis represents the correlation of the receptor expression alone with tumor growth rate. Points are colored according to the strength of correlation of the interaction scores with tumor growth rate. Gray points represent interactions that were not detected across all syngeneic tumor models.

Article Snippet: LL2 cancer cell line , ATCC , CRL-1642.

Techniques: Expressing

Journal: Cell reports

Article Title: Analysis of Single-Cell RNA-Seq Identifies Cell-Cell Communication Associated with Tumor Characteristics

doi: 10.1016/j.celrep.2018.10.047

Figure Lengend Snippet:

Article Snippet: LL2 cancer cell line , ATCC , CRL-1642.

Techniques: Software