Journal: Nature Communications

Article Title: PRKCSH contributes to tumorigenesis by selective boosting of IRE1 signaling pathway

doi: 10.1038/s41467-019-11019-w

Figure Lengend Snippet: The level of PRKCSH is increased in various cancer tissues. a Map of human organs in which the expression of PRKCSH mRNA is significantly higher in tumors than in nontumor. Gene-expression datasets from TCGA were analyzed by using the GEPIA web tool. b Scatter plots showing relative levels of PRKCSH mRNA in nontumor and tumor tissues. Median expression levels in each group are indicated by horizontal lines and these values are shown in ( a ). One-way ANOVA; * P < 0.01. c Representative immunohistochemical images, and d relative levels of PRKCSH protein. The intensity of PRKCSH staining in paraffin-embedded sections of liver tissue arrays (nontumor, n = 59; tumor, n = 58) was calculated as described in Supplementary Fig. . Scale bars represent 20 µm. The center line of the boxplots denotes the median, the bounds of the box indicate 25–75% and the whiskers represent 5–95%, respectively. Significance of the differences between the two categories was determined by the Student t test ( P < 0.0001). e Immunoblot analysis of PRKCSH expression in human liver cell lines. f Relationship between PRKCSH expression and clinicopathological features. Among the clinicopathological data of the liver tissue array, extrahepatic metastasis and TNM stage were positively related to PRKCSH staining (Supplementary Table ). g Overall survival and disease-free survival curves for patient groups with high and low PRKCSH expression level. The data were obtained from TCGA datasets. Significance of the differences between the two categories was determined by Log-rank test

Article Snippet: Liver tissue array slides were purchased from SuperBioChips Laboratories and used to determine the levels of PRKCSH by IHC analysis.

Techniques: Expressing, Gene Expression, Immunohistochemical staining, Staining, Western Blot

Journal: Journal of Cancer

Article Title: TGF-β Induces Degradation of PTHrP Through Ubiquitin-Proteasome System in Hepatocellular Carcinoma

doi: 10.7150/jca.10830

Figure Lengend Snippet: Tissue array analysis of TGF-β and PTHrP expression in HCC and non-cancerous specimens. A human tissue array containing 38 HCC cancer tissues and 9 non-neoplastic liver tissues was double-stained for PTHrP and TGF-β by immunohistochemistry using the Poly-HRP Anti-Mouse IgG Detection System (brown staining - TGF-β) and the Biotin SP-AP Detection System (blue staining - PTHrP), respectively. The nuclei were counterstained with nuclear fast red. (A) A representative image showing PTHrP staining but no TGF-β staining in the HCC cancer cells in a HCC patient (ID #08). (B) TGF-β was intensely stained in the matched non-neoplastic liver tissue of the same patient. (C) PTHrP expression level was considerably higher in HCC tissues than in the non-neoplastic liver tissues. (D) TGF-β expression was high in the non-neoplastic liver tissues but low in HCC cancer tissues. Original magnification: x400.

Article Snippet: Liver cancer tissue array slide (IMH-360) including 38 cases of HCC and combined HCC & cholangiocarcinoma samples and 9 matched non-neoplastic liver tissues was purchased from Novus (Oakville, ON, Canada).

Techniques: Expressing, Staining, Immunohistochemistry